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Serological diagnosis of human polyomavirus infection

Measurement of antibody titres to the human polyomaviruses BK and JC has for many years had to rely on Hemagglutination inhibition. In recent years, viral serology based on virus-like particles (VLPs) in enzyme immunoassays (EIAs) has become widely used for a variety of viruses. We sought to establish a modern method for serological diagnosis of BK and JC viruses, by using purified VLPs containing

The Big Bang of History : Visualism in Technoscience

The traditional presentation about historical time-passing consists in a linear succession of facts in which some aspects of the life world evolve from others in an irreversible manner. The presentation of change is connected to the presentation of gradual or revolutionary linear changes that are irrevocable. I believe that this presentation could be considered correct for living organisms, but do

Mechanisms underlying neoplasia-associated genomic rearrangements

Neoplastic disorders are characterized by recurrent somatically acquired chromosomal aberrations that alter the structure and/or expression of a large number of genes. Most “cancer genes” discovered to date in human neoplasms have been identified through isolation of genes at the breakpoints of balanced chromosomal translocations. Although functional studies of such cancer-causing genes have demon

Lifetime measurements for excited states of rare-earth atoms using pulse modulation of a cw dye-laser beam

By pulse-modulating a cw dye-laser beam and employing the delayed-coincidence technique, we have determined the natural radiative lifetimes of a number of atomic states in rare-earth atoms Eu, Dy, and Yb. In the measurements, which were performed on an atomic beam, exponential decay curves with good statistics were obtained using short measuring times. The general features of the technique are dis

The human CD77(-) B cell population represents a heterogeneous subset of cells comprising centroblasts, centrocytes, and plasmablasts, prompting phenotypical revision

The process of becoming an Ig-producing plasma cell takes the mature B cell through the germinal center, where Ig genes are diversified through somatic hypermutation and class switch recombination. To more clearly define functional characteristics of the germinal center dark zone centroblasts and the light zone centrocytes, we have performed expression analysis of the CD77(+) and CD77(-) populatio

Guided wave propagation in three-layer pavement structures

A study on guided waves in a. layered half-space with large velocity contrasts and a decreasing velocity with depth is presented. Multiple mode dispersion curves are calculated in the complex wave number domain, taking into consideration the attenuation caused by leakage into the underlying half-space. The excitability of the modes by a vertical point force on the surface is also calculated. Resul

Novel immunodeficiency data servers

The Internet contains scientific information in increasing amounts. It is possible to obtain the latest information, and Web services can easily be maintained and updated. We have set up three Internet ser vices an immunodeficiencies. Immunodeficiency-related mutation information is available in immunodeficiency mutation databases (IDbases). Currently 14 registries are distributed, including infor

Antigen-binding sites dominate the surface properties of antibodies

We have found a remarkable relationship between the specificity of antibodies and their chromatographic behavious upon liquid-liquid partition chromatography (LLPC). Well characterized human and murine monoclonal antibodies and Fab/Fv fragments thereof as well as mouse/human chimeric antibodies were employed. While, lgG 1, 2 and 4 antibodies with identical specificities (affinity constants) have i

The acoustical significance of tongue, lip, and larynx maneuvers in rounded palatal vowels

The acoustical consequences of articulatory maneuvers of [y] are studied in model experiments in order to obtain insights into articulator programming and speech motor control by elucidating the role of each component maneuver of a speech segment in setting up vocal tract resonance conditions for the spectral features of the speech wave. The maneuvers of [y] are found to provide a maximum and stab