Sökresultat

The effect of irradiation on S-phase duration (Ts), labelling index (LI), potential doubling time (Tpot), and cell cycle phase distributions was determined by DNA flow cytometry in xenografted human squamous cell carcinoma of the head and neck (SCCHN). Tumours were treated with a single dose of 3 Gy, and excised at intervals over a 90-h period. Six hours before each excision the tumours were label

Surface exposed cysteines were genetically engineered in the structure of recombinant horseradish peroxidase (rHRP). Recombinant forms of HRP with either a His-tag or a Strep-tag at the C-terminus were produced, which additionally had cysteines at positions 57, 189 or 309 (C-terminus) of the polypeptide chain. An E coli expression system was exploited. The effect of these mutations on the direct e

The aim of the present study was to examine the effects of wearing a light-weight ice-vest (1 kg, water) on physiological and subjective responses in fire fighter’s work. The experiments were carried out in a climatic chamber, in a container under extreme radiant heat, and during simulated smoke-diving. In addition, the physical cooling effect of the ice-vest was measured with a thermal mannequin.

Context: SHBG regulates free sex steroid levels, which in turn regulate skeletal homeostasis. Twin studies have demonstrated that genetic factors largely account for interindividual variation in SHBG levels. Glucuronidated androgen metabolites have been proposed as markers of androgenic activity. Objective: Our objective was to investigate whether polymorphisms in the SHBG gene promoter [(TAAAA)(n

Background and Purpose - Regular endurance exercise has been shown to reduce the age-related increase in arterial stiffness that is thought to contribute to cardiovascular risk. The aim of this study was to evaluate the influence of age and habitual physical activity on carotid artery wall thickness and stiffness in a population of young to middle-age subjects at low cardiovascular risk. Methods -

Background: The 39 item Parkinson's disease questionnaire (PDQ-39) is the most widely used patient reported rating scale in Parkinson's disease. However, several fundamental measurement assumptions necessary for confident use and interpretation of the eight PDQ-39 scales have not been fully addressed. Methods: Postal survey PDQ-39 data from 202 people with Parkinson's disease (54% men; mean age 70

Introduction. There are few published guidelines for the management of sexual dysfunctions in men and women, despite the prevalence and lack of attention to these problems. Disorders of sexual function in men include erectile dysfunction, orgasm/ejaculation disorders, priapism, and Peyronie's disease. Aim. To provide evidence-based and expert-opinion consensus guidelines for the clinical managemen

Background Exposure to Benzodiazepines (BZD) during foetal life has been suggested to contribute to neonatal morbidity and some congenital malformations, for example, orofacial clefts. Here we aimed to study the neonatal outcome and congenital malformations in neonates whose mothers reported use of BZD and/or hypnotic benzodiazepine receptor agonists (HBRA) during pregnancy. Methods In the Swedish

1. Recent changes in European agricultural policy have led to measures to reverse the loss of species-rich grasslands through the creation of new areas on ex-arable land. Ex-arable soils are often characterized by high inorganic nitrogen (N) levels, which lead to the rapid establishment of annual and fast-growing perennial species during the initial phase of habitat creation. The addition of carbo

Low molecular weight protein tyrosine phosphatase (LMW-PTP) dimerizes in the phosphate-bound state in solution with a dissociation constant of K-d = 1.5(+/-0.1) mM and an off-rate on the order of 10(4) s(-1).H-1 and N-15 NMR chemical shifts identify the dimer interface, which is in excellent agreement with that observed in the crystal structure of the dimeric S19A mutant. Two tyrosine residues of

Objectives: Phosphodiesterase 5 (PDE5) inhibitors improve smooth muscle relaxation and therefore are considered for pharmacotherapy of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Cyclic guanosine monophosphate (cGMP) -dependent protein kinase-1 (cGKI) has been identified as one of the downstream targets for cGMP. The aim of the present study was to evaluate, by mean

Vascular smooth muscle tone is dependent on oxidative metabolism, a phenomenon of potential importance for the metabolic regulation of blood flow to tissues. The response of the rat portal vein to inhibition of cell respiration by cyanide (0.1-1 mM) is a reduction of its spontaneous myogenic activity. The trains of action potentials triggering phasic contractions are reduced in duration, while the

Cytogenetic analyses of four squamous cell carcinomas (SCC) of the esophagus showed complex numerical and structural abnormalities. Chromosomal bands or regions preferentially involved were 11q13, 8q10, 21q10, 3p10similar top11, 1p11similar toq11, 5p11similar toq11, and 14p11similar toq11. For the first time, to our knowledge, recurrent aberrations were identified in esophageal SCC, including homo

Lack of carbon has been assumed to be the most common limiting factor for bacterial growth in soil, although there are reports of limitation by other nutrients, e.g. nitrogen and phosphorus. We have studied which nutrient(s) limited instantaneous growth rates of bacteria in 28 Swedish soils using the thymidine or leucine incorporation technique to measure increased growth rate after adding differe

BACKGROUND AND PURPOSE: Although the incidence of and mortality from myocardial infarction (MI) have declined in most industrialized countries, incidence studies of stroke have shown less consistent trends. This study examines time trends in the incidence of stroke and case fatality rates in relation to socioeconomic circumstances and history of MI.METHODS: Stroke incidence in the city of Malmö, S

1 The mechanisms behind bradykinin-induced effects in the airways are considered to be largely indirect. The role of cholinergic nerves and eicosanoids, and their relationship in these mechanisms were investigated in guinea-pigs. 2 The role of cholinergic nerves was studied in animals given atropine (1 mg kg-', i.v.), hexamethonium (2 mg kg-', i.v.), or vagotomized. To study the role of eicosanoid

Epithelial ovarian cancer (EOC) is the most lethal of all gynaecological malignancies and there is an urgent need to develop new prognostic and treatment predictive biomarkers for a more efficient therapy. The discovery of the RNA-binding motif protein 3 (RBM3) as a putative cancer biomarker was based on its differential expression in various cancer forms in the Human Protein Atlas (HPA). Further

Objective To study the characteristics of the infants born to women who have used anti-asthmatic drugs during pregnancy. Material and methods Various characteristics of infants with mothers who had reported the use of anti-asthmatic drugs in early pregnancy (n=24,750) or had such drugs prescribed later during pregnancy by a maternity health care centre (n=763) were identified from the Swedish Medi

Alkaline sphingomyelinase (alk-SMase) is a new member of the NPP (nucleotide pyrophophatase/phosphodiesterase) family that hydrolyses SM (sphingomyelin) to generate ceramide ill the intestinal tract. The enzyme may protect the intestinal mucosa front inflammation and tumorigenesis. PAF (platelet-activating factor) is a pro-inflammatory phospholipid involved in pathogenesis of inflammatory bowel di