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From initial hit to crystal optimization with microseeding of human carbonic anhydrase IX—A case study for neutron protein crystallography

Human carbonic anhydrase IX (CA IX) is a multi-domain membrane protein that is therefore difficult to express or crystalize. To prepare crystals that are suitable for neutron studies, we are using only the catalytic domain of CA IX with six surface mutations, named surface variant (SV). The crystallization of CA IX SV, and also partly deuterated CA IX SV, was enabled by the use of microseed matrix

Design for deconstruction in the design process : State of the art

Stricter building regulations have resulted in the construction of buildings with a low energy use during the operation phase. It has now become increasingly important to also look at the embodied energy, because it might, over the lifespan of the building, equal the energy used for operating the building. One way to decrease the embodied energy is to reuse building materials and components or to

User Assignment with Distributed Large Intelligent Surface (LIS) Systems

In this paper, we consider a wireless communication system where a large intelligent surface (LIS) is deployed comprising a number of small and distributed LIS-Units. Each LIS-Unit has a separate signal process unit (SPU) and is connected to a central process unit (CPU) that coordinates the behaviors of all the LIS-Units. With such a LIS system, we consider the user assignments both for sum-rate a

A fluorescence study of the loading and time stability of doxorubicin in sodium cholate/PEO-PPO-PEO triblock copolymer mixed micelles

HypothesisDoxorubicin hydrochloride (DX) is one of the most powerful anticancer agents though its clinical use is impaired by severe undesired side effects. DX encapsulation in nanocarrier systems has been introduced as a mean to reduce its toxicity. Micelles of the nonionic triblock copolymers of poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO) (PEO-PPO-PEO), are very promising carrier