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Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions

DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease

Early Holocene terrestrial climatic variability along a North Atlantic Island transect: palaeoceanographic implications

A synthesis of the early Holocene climatic development in the North Atlantic region is presented, based on three previously published lake records from southern Greenland (Lake N14), Iceland (Lake Torfadalsvatn) and the Faroe Islands (Lake Lykkjuvotn). The interval 11500-8500 cal BP has been divided into five phases with respect to the inferred strength of the North Atlantic Current (NAC) and Irmi

Characterization of behavioral and neurodegenerative changes following partial lesions of the nigrostriatal dopamine system induced by intrastriatal 6-hydroxydopamine in the rat

Partial lesions of the nigrostriatal dopamine system have been investigated with respect to their ability to induce consistent long-lasting deficits in movement initiation and skilled forelimb use. In eight different lesion groups 6-hydroxydopamine (6-OHDA) was injected at one, two, three, or four sites into the lateral sector of the right striatum, in a total dose of 20-30 microgram. Impairments

Thermostable carbohydrate-binding modules in affinity chromatography

Affinity chromatography is routinely used mostly on a preparative scale to isolate different biomolecules such as proteins and carbohydrates. To this end a variety of proteins is in common use as ligands. To extend the arsenal of binders intended for separation of carbohydrates, we have explored the use of carbohydratebinding modules (CBM) in affinity chromatography. The thermostable protein CBM4-

Hidden Markov model-based packet loss concealment for voice over IP

As voice over IP proliferates, packet loss concealment (PLC) at the receiver has emerged as an important factor in determining voice quality of service. Through the use of heuristic variations of signal and parameter repetition and overlap-add interpolation to handle packet loss, conventional PLC systems largely ignore the dynamics of the statistical evolution of the speech signal, possibly leadin

Decision Support for the Initial Triage of Patients with Acute Myocardial Infarction

Objectives: To develop an automated tool for the analysis of electrocardiograms (ECG) with respect to changes that make the patient a candidate for reperfusion therapy. An additional aim was to assess the influence of the tool on the ECG classifications of three interns. Methods and Results: An artificial neural network was trained to interpret ECGs regarding changes making the patient a candi

The meaning of psychosocial occupational therapy in a life-story perspective. A long-term follow-up of three cases.

This study is a long-term follow-up in narrative form of three former psychiatric patients who had been discharged eight to nine years earlier from an outpatient occupational therapy programme. The purpose of the study was to gain an understanding about the participants' views about the period of therapy from a long-term perspective and of how the outcome of therapy could be understood within the

Long-term temporal trends in cardiovascular and metabolic risk factors

OBJECTIVES: Metabolic factors such as obesity, hypertension, dyslipidemia and hyperglycemia have consistently been associated with increased risk of cardiovascular disease. There is also growing evidence that these factors are linked to cancer incidence and mortality. The aim of this study was to investigate long-term trends in major metabolic risk factors in three large cohorts. MATERIALS AND MET

Experimental evidence for sub-3-fs charge transfer from an aromatic adsorbate to a semiconductor

The ultrafast timescale of electron transfer processes is crucial to their role in many biological systems and technological devices. In dye-sensitized solar cells(1-4), the electron transfer from photoexcited dye molecules to nanostructured semiconductor substrates needs to be sufficiently fast to compete effectively against loss processes and thus achieve high solar energy conversion efficiencie

Cryptochromes and activity markers co-localize in bird retina during magnetic orientation

Migratory birds can use a magnetic compass for orientation during their migratory journeys covering thousands of kilometers. But how do they sense the reference direction provided by the Earth's magnetic field? Behavioral evidence and theoretical considerations have suggested that radical-pair processes in differently oriented, light-sensitive molecules of the retina could enable migratory birds t

Normal ultrasonic fetal growth ratios evaluated in cases of fetal disproportion

During a 2-year period, 5476 normal routine obstetrical ultrasound investigations were performed in the 2nd trimester (16th to 20th week). Data on biparietal diameter (BPD), abdominal diameter (AD) and femur length (FL) have been obtained from this material. Ratios between BPD/AD and BPD/FL have been calculated, and from these ratios, graphs were constructed. Only nine normal fetuses (0.2%) were f

Using burden of disease/cost-effectiveness as an instrument for district health planning: experiences from Uganda.

The burden of disease/cost-effectiveness analysis (BoD/CE) was introduced as a method for detailed planning and budgeting in 13 districts of Uganda. This endeavor can be seen as a “natural experiment”, attempting to pursue policy implementation by means of a heavy focus on rationalistic/technical arguments as a main supportive factor. However, modern theory of policy implementation, e.g. the new i

Radiation-induced increase in hyaluronan and fibronectin in bronchoalveolar lavage fluid from breast cancer patients is suppressed by smoking

Bronchoalveolar lavage (BAL) fluid was analysed from 21 patients with breast cancer, stage T1N0M0, who had undergone tumour resection and post-operative local irradiation (accumulated dose 56 Gy). The lavage was performed two months after radiotherapy, in the anterior part of the lingula (left side) or of the right middle lobe (right side), depending on which side had been exposed to radiation. Th

Effect of particle size on biogas yield from sisal fibre waste

The degradation and biogas production potential of sisal fibre waste could be significantly increased by pre-treatment for reduction of particle size. Batch-wise anaerobic digestion of sisal fibre waste was carried out in 1-l digesters with fibre sizes ranging from 2 to 100 mm, at an ambient temperature of 33 °C. Sediment from a stabilisation pond at a sisal production plant was used as starter se

A night vision brain area in migratory songbirds

Twice each year, millions of night-migratory songbirds migrate thousands of kilometers. To find their way, they must process and integrate spatiotemporal information from a variety of cues including the Earth's magnetic field and the night-time starry sky. By using sensory-driven gene expression, we discovered that night-migratory songbirds possess a tight cluster of brain regions highly active on

Treatment potential for cholesterol management in patients with coronary heart disease in 15 European countries: Findings from the EUROASPIRE II survey

Background: During the last decade, the evidence of beneficial effects of cholesterol lowering in patients with coronary heart disease (CHD) has been proven in several clinical trials. This has prompted international guidelines on prevention of CHD to include recommendations on dietary and pharmacological treatment of hyperlipidaernia with set goals on total- and LDL-cholesterol. Methods: The firs

Global patterns of gene regulation associated with the development of ectomycorrhiza between birch (Betula pendula Roth.) and Paxillus involutus (Batsch) fr.

The formation of ectomycorrhizal (ECM) root tissue is characterized by distinct morphological and developmental stages, such as preinfection and adhesion, mantle, and Hartig net formation. The global pattern of gene expression during these stages in the birch (Betula pendula)-Paxillus involutus ECM association was analyzed using cDNA microarrays. In comparison with nonsymbiotic conditions, 251 fun

Cationic amphiphilic polyelectrolytes and oppositely charged surfactants at the silica-aqueous interface

The influence of sodium dodecyl sulfate (SDS) on the interfacial behavior of two amphiphilic polyelectrolytes, which are copolymers of the cationic monomers triethyl(vinylbenzyl)ammonium chloride and dimethyldodecyl(vinylbenzyl)ammonium chloride, at the silica-aqueous interface was studied. The fraction of amphiphilic monomers was varied, where ODT, 40DT, and 80DT contained 0, 40, and 80 mol % mon