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Miniaturized thermal biosensors based on three different designs have been constructed. Properties relevant to decentralized bioanalysis have been investigated. A short measurement period (30 s per sample) and a broad linear range (0.5 mM to 100 mM for glucose and penicillin-V), using 1 μl sample volume, have been achieved.
Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. T
Malignant breast tumours can be distinguished from benign lesions and normal tissue based on their mechanical properties. Our pilot studies have demonstrated the potential of using Mechanical Imaging (MI) combined with mammography to reduce recalls and false positives in breast cancer screening by more accurately identifying benign lesions. To enable further optimization of MI we propose a compute
A biosensor that combines electrochemistry and calorimetry was investigated. Using this sensor, glucose measurements were performed by employing glucose oxidase together with ferrocene as electron-transfer mediator. The electrochemical reaction was accomplished by applying a voltage between a platinum column (working electrode), in contact with a crushed reticulated vitreous carbon (RVC) matrix on
In order to develop a sensitive, versatile and inexpensive portable instrument, a calorimetric micro-biosensor was designed and fabricated by etching part of the flow system into a silicon wafer. The utility of the device was tested using the enzymes, catalase and penicillinase. This sensor showed good reproducibility and linearity up to 100 mM. The system also showed higher sensitivity than the c
New applications of a flow-calorimetric biosensor system, the enzyme thermistor (ET) are described. The ET measures the reaction heat in a small column containing immobilized biocatalyst as a temperature change of the effluent from the column. Since most biological reactions are exothermic, the ET has a very broad applicability and numerous assays of clinical as well as biotechnological interest h
A newly designed thermal biosensor (TBS) is reported. It is constructed by integrating a temperature sensor with a bioreactor, which can be easily dismantled. Such a design makes the TBS versatile. Both differential and adiabatic techniques are used to improve its precision and resolution. As an example of application, a glucose assay is described: resolution 2 × 10-8 mol, linear range 2 × 10-8 mo
The invention discloses an optical circulation enlarged type biological chip detecting method, wherein the enzyme recirculation and bioluminescence are combined, for realizing the FMNH#-[2] accumulation through the circulation on the electrodes by the NADH/NAD#+[+] oxidation-reduction, the high level accumulation FMNH#-[2] reacts with other light-emitting bottom luciferase, long chain aldehydes an
A method of manufacturing a protein chip for identifying aminoacyl-tRNA synthetases, suitable for detecting basic amino acids in proteins. It is characterized in, immoblizing aminoacyl-tRNA synthetases corresponding to 20 basic amino acids in arrays onto the surface of the chip, then fixing 20 kinds of tRNAs labelled by fluorescence onto the corresponding synthetases, then obtaining a chip of amin
The unsurpassed versatility of heat sensitive biosensors is illustrated by various new applications of the flow-calorimetric biosensor system, the enzyme thermistor (ET) developed in our laboratory. The ET measures the reaction heat in a small column containing immobilized biocatalyst. Since most biological reactions are exothermic, the ET has a very broad applicability and numerous assays of clin
An integrated thermal biosensor array was designed and fabricated for the simultaneous determination of glucose, urea and penicillin. The sensor contains ten thin-film thermistors which were fabricated on quartz chip by micromachining and semiconductor technologies. This sensor allows to determine maximum five different analytes nearly simultaneously by serially dividing the single microchannel in
Background: Patients presenting with synchronous colorectal liver metastases are increasinglybeing considered for a curative treatment, and the liver-first approach is gaining popularity in thiscontext. However, little is known about the completion rates of the liver-first approach and itseffects on survival.Methods:A systematic review and meta-analysis of liver-first strategy for colorectal liver
A summary of our efforts to develop miniaturized biosensors based on the enzyme thermistor is presented. Three constructions are described. The work focuses on the measurement of glucose in whole blood.The first biosensor was used to analyze concentrated and tenfold diluted blood samples. The glucose concentration obtained using this construction correlated well with the reference method.Using the
Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- a
This article develops a more comprehensive understanding of innovation-based renewal of industries from a structural perspective. Arguably, established perspectives offer rather simplistic views, portraying structures as either enabling or constraining for certain forms of regional industrial change. Inspired by work in organizational institutionalism on ‘institutional infrastructures’, this artic
This dissertation studies the labor market development of fourteen Western European countries during the years 1970-90, focusing on the relatively low Swedish unemployment. The variation in unemployment over time and between countries has proved difficult to explain with the most influential theoretical models, which are usually categorized as New-keynesian and Post-keynesian respectively. The dis
