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Enzymatic condensation between amino acids and monosaccharides was studied. A screening involving the OH-containing amino acids serine and threonine and the monosaccharide substrates for the enzymes α-N-acetylgalactosaminidase, α-galactosidase, α-mannosidase, β-xylosidase, and β-galactosidase was carried out. Enzymatic condensation was observed with α-mannosidase and α-N-acetylgalactosaminidase. P
New methods and applications in the separation of biomolecules are reviewed, with an emphasis on the large-scale recovery of proteins. Highlights include the advent of flow-through particles in perfusion chromatography, which allows for very high flow rates, while retaining a high chromatographic efficiency.
ObjectiveTo compare and evaluate early and midterm outcomes of a “no cross” novel approach with short tip vs. standard tip introducer systems for all distal components to prevent target vessel bridging stent kink or collapse during fenestrated endovascular aortic repair (FEVAR).MethodsA retrospective analysis was conducted on all patients who underwent FEVAR at a tertiary referral centre between O
Saturated heterocycles are found widely in biologically active compounds such as medicinal drugs and agrochemicals. However, boronic acid-derived building blocks for these structures have limited availability, particularly in comparison to heteroaromatic boronic acids. We report the preparation of boronic ester γ-lactams through a Cu-catalysed conjugate borylation-cyclisation protocol. Using a chi
Liskov, Rivest and Wagner laid the theoretical foundations for tweakable block ciphers (TBC). In a seminal paper, they proposed two (up to) birthday-bound secure design strategies — LRW1 and LRW2 — to convert any block cipher into a TBC. Several of the follow-up works consider cascading of LRW-type TBCs to construct beyond-the-birthday bound (BBB) secure TBCs. Landecker et al. demonstrated that j
Objective: This study evaluated, in a Swedish setting, the cost effectiveness of fenfluramine (FFA) as an add-on to standard of care (SoC) for reducing seizure frequency in Dravet syndrome, a severe developmental epileptic encephalopathy. Methods: Cost effectiveness of FFA+SoC compared with SoC only was evaluated using a patient-level simulation model with a lifetime horizon. Patient characteristi
Despite the increasing challenges twenty-first-century societies face in accommodating older adults, many misconceptions about old age before modernity continue to exist. These are rarely expressed in ‘formal theory’ produced by academics, but rather persist in ‘lay theory’ which is anchored in collective memory and popular culture. Such taken-for-granted beliefs are nevertheless influential, firs
Lactate dehydrogenase (LDH) was recovered from a solution by affinity binding to an N6-(6-aminohexyl)-AMP-Sepharose gel. An enzyme thermistor unit was employed to continously measure the activity of the unbound LDH. The enzyme activity signal from the enzyme thermistor was used in a PID controller to regulate the addition of AMP-Sepharose gel to the LDH solution. In another type of experiment, a d
On-line monitoring of chromatographic procedures involving biomolecules is usually restricted to UV registration and determination of the pH or conductivity of the mobile phase. A more specific monitoring of proteins, for instance direct identification of a special enzyme, would greatly facilitate and speed up purification. We have found that flow calorimetry, performed with an enzyme thermistor i
Magnetic aqueous two-phase separation is a new technique to speed up the separation of aqueous two-phase systems (Anal. Biochem. 1987, 167, 331–339). It is based on the addition of magnetically susceptible material (e.g. 1-μ iron oxide particles) which induces rapid phase separation when a mixed system is placed in a magnetic field. The technique has been applied to a number of two-phase systems.
The effects of peptidolysis during early cheese ripening were studied in a low-fat (10% fat), round-eyed, semi-hard cheese using gel permeation chromatography and determinations of N content in cheese extracts. Addition of a heat-treated culture of Lactobacillus helveticus enhanced the peptidolytic activity even during the first weeks of cheese ripening. An aminopeptidase was found to have the nec
The reversal of the hydrolytic activity of α-mannosidase from jack bean has been studied in high concentrations of mannose. From an initial concentration of 85% mannose (w/w), a maximum yield of 70% (w/w) di- and oligosaccharides was obtained. The products were separated by gel chromatography and high-performance liquid chromatography, and characterized by gas-liquid chromatographymass spectrometr
β-d-Galp-(1→3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then ga
This work employs ambient pressure X-ray photoelectron spectroscopy (APXPS) to delve into the atomic and electronic transformations of a core-shell Ni@NiO/NiCO3 photocatalyst - a model system for visible light active plasmonic photocatalysts used in water splitting for hydrogen production. This catalyst exhibits reversible structural and electronic changes in response to water vapor and solar simu
Galβ1-3GlcNAc (1) and Galβ1-3GlcNAcβ-SEt (2) were synthesized on a 100 mg scale by the transgalactosylation reaction of bovine testes β-galactosidase with lactose as donor andN-acetylglucosamine and GlcNAcβ-SEt as acceptors. In both cases the product mixtures contained unwanted isomers and were treated with β-galactosidase from Escherichia coli which has a different specificity, under conditions f
IntroductionPrevious studies show that approximately 40% of hospital readmissions in older adults are medication-related and that about 70% of these could be prevented. A risk assessment tool aiming to identify older adults at increased risk of medication-related readmission could help maximize the impact of implemented preventive measures and save healthcare resources. In this study we made furth
This entry offers a short description of basic research questions, different theories, and topics in lexical semantic research. At the beginning, it states five fundamental questions that all lexical semantics theories need to attend to in descriptions and explanations of their observations and findings. A brief presentation of past and present theoretical approaches on lexical semantics follows,
This special issue of the journal Urban Matters on “Intermedial Interventions in the City” explores the complexity of urban media life by adressing such questions as: how do intermedial processes shape, conceptualise, and otherwise intervene in the city? How do different kinds of media combinations produce and contest urban space? How are intermedial performances integrated into the social and po
