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Two oligonucleotide primers were used for selective enzymatic amplification of a DNA segment encoding a major portion of the first constant region domain (CH1) of the human IgG1 heavy chain. The selective amplification was confirmed by use of subclass-specific oligonucleotide probes. Two 15-mer oligonucleotides, hybridizing with the alleles for the allotypes G1m(f) and (z), respectively, could the
While the use of radio technology for wireless data communications has increased rapidly, the wide variety of radio interfaces being used has made interference investigations hard to perform. With that in mind, we present a novel approach for analyzing packet radio communications, applicable to interfering heterogeneous networks, which leads to tractable analytical expressions. The core of the app
Organic substrates for pelagic bacteria are derived from dissolved organic carbon (DOC) in the water column. DOC is a heterogeneous mixture of molecules, some of which are imported from the watershed (allochthonous DOC) and others that are produced by autotrophs within the system (autochthonous DOC). We examined the importance of autochthonous versus allochthonous DOC in supporting the growth of p
Computer programming should be expressing the complicated in easily understandable parts. General languages provide tools and abstractions that allow many different problems to be formulated and solved. Unfortunately, these abstraction only rarely match the problem area precisely, resulting in solutions that are obscured by the need for support code unrelated to the problem. This can be avoided, b
In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramolecular charge transfer (ICT) state in the peridinin excited state manifold. The I
This study is motivated by the understanding that entrepreneurship varies from one context to another as there are distinctive factors that influence the development and organization of entrepreneurial activities in any particular national context. Using qualitative methodology the study aims at developing a conceptual and empirical framework for the study and understanding of the phenomenon of en
Using sticky traps baited with (2S, 3S, 7S)-3,7-dimethyl-2-pentadecyl acetate, we assessed the seasonal and diurnal flight activity of the European pine sawfly, Neodiprion sertifer (Geoffrey), in Fayette, Madison, and Livingston counties in Kentucky. At each site, two replicates of individual sticky traps were baited and suspended about 1 m high and spaced at least 20 m apart. Trap catch was affec
Popular Abstract in Swedish Avhandlingen handlar om hur radiovågor tar sig fram i miljöer där saker som hus, väggar och fönster är i vägen. Framför allt ligger fokus på hur signalen från en sändare placerad utomhus kan nå fram till en mobil terminal (t. ex. en mobiltelefon) som befinner sig inomhus. Hur mycket signalen dämpas när den passerar väggen är av stor betydelse när man ska avgöra hur långThis thesis deals with radio wave propagation in environments where the propagation is severely influenced by surrounding objects, mainly walls and other building structures. As an introduction, a brief description is given on propagation terms like loss, delay and dispersion along with system functions relevant for the topic. The focus is then placed on propagation from an outdoor transmitter to
Background: The use of global gene expression profiling to identify sets of genes with similar expression patterns is rapidly becoming a widespread approach for understanding biological processes. A logical and systematic approach to study co-expressed genes is to analyze their promoter sequences to identify transcription factors that may be involved in establishing specific profiles and that may
Steady-state and transient-state kinetic experiments have been performed to test the proposal that there is a direct (channelled) transfer of NADH from glyceraldehyde-3-phosphate dehydrogenase to alcohol dehydrogenase. The results lend no support to this proposal, but can be best explained in terms of a free-diffusion mechanism for NADH transfer between the two enzymes.
Purpose: To describe the phenotype using electroretinography and optical coherence tomography (OCT) in members of two families with different mutations in RDS. Methods: DNA was extracted from blood samples and used for mutation screening by denaturing gradient gel electrophoresis (DGGE) and nucleotide sequencing of RDS exons. Patients were examined with clinical evaluation, full-field electroreti
