Production of recombinant TEV protease in Escherichia coli and analysis of enzymatic reactions
Although different types of affinity tags have become a tool in the production and purification of recombinant proteins in various applications, the use of these have the risk of interfering with the biological activity and crystallization of proteins.There are both chemical and enzymatic ways to remove these tags, the enzymes include enteropeptidase, thrombokinase and protease to mention a few. T
