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This thesis focuses on the characterization of Coniferyl aldehyde dehydrogenase (CALDH) and cofactor regeneration involved in the biotransformation process from 5-Formyl-2-furancarboxylic Acid (FFCA) to 2,5-furandicarboxylic acid (FDCA) using NADH oxidases. FDCA is a building block of bio-based polymers like polyethylene furanoate (PEF), which serves as a sustainable alternative to traditional Pol
