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Both freezing tolerance and NaCl tolerance are improved when antifreeze proteins are expressed as fusion proteins with two domains of staphylococcal protein A (SPA) in Escherichia coli. To characterize these properties further we created a randomly mutated expression library in E. coli, based on the winter flounder antifreeze protein HPLC-8 component gene. Low-fidelity PCR products of this gene we
Proteins carrying genetically attached polyhistidine tails have been purified using affinity precipitation with metal chelates. DNA fragments encoding four or five histidine residues have been genetically fused to the oligomeric enzymes lactate dehydrogenase (Bacillus stearothermophilus), beta-glucoronidase (Escherichia coli), and galactose dehydrogenase (Pseudomonas fluorescens) as well as to the
To examine the role of the connecting region in the artificial bifunctional enzyme beta-galactosidase/galactose dehydrogenase, linkers of different length were inserted between the catalytic units. The specific activity of the galactose dehydrogenase part of the complex was increased when longer linkers (9 and 13 amino acids) were used as connectors. These bifunctional enzymes were predominantly f
We report the design of a cyclic, eight-residue peptide that possesses the catalytic triad residues of the serine proteases. A manually built model has been relaxed by 0.3 ns of molecular dynamics simulation at room temperature, during which no major changes occurred in the peptide. The molecule has been synthesised and purified. Two-dimensional NMR spectroscopy provided 35 distance and 7 torsion
We have expressed the DNA of the fusion of CS1 to MDH1 in Escherichia coli gltA-. The fusion protein (CS1/MDH1) is the C-terminus of CS1 linked in-frame to the N-terminus of MDH1 with a short linker of glycyl-seryl-glycyl. The fusion protein produced was isolated and purified. Gel filtration studies indicated that CS1/MDH1 had a M(r) of approximately 170,000. Western blotting analysis with SDS gel
För att åstadkomma en förändrad färdmedelsfördelning krävs kunskap om olika gruppers preferenser och deras faktiska beteende. Syftet med denna rapport är att identifiera arbetspendlares preferenser som i förlängningen kan ligga till grund för planering av ökat gående och cyklande. Studien tar sitt avstamp i en webbaserad resvaneundersökning ställd till anställda på Lunds Tekniska Högskola (LTH), M
Vi lever i en tid av snabb vetenskaplig och teknologisk utveckling. För 100 år sedan hade ingen kunnat drömma om de saker som helt har förändrat vår var- dag och hur vi uppfattar omvärlden; datorer, mobiltelefoner, Internet, rymdfart, våra upptäckter om universums barndom och avlägsna galaxer samt vår insyn i kvantfenomenens mikroskopiska värld. Idag står vi på tröskeln till en era formad av nanotWe live in a time of rapid scientific and technological advancement. People liv- ing 100 years ago could never dream of inventions like those having completely changed our way of life, and our perception of the world; computers, mobile phones, the Internet, space travel, unraveling the mysteries of the early universe and distant galaxies, and our insight into the microscopic world of quantum phe-
Stefan Larsson’s Conceptions in the Code makes a significant contribution to sociolegal analysis and also represents a valuable contribution to conceptual metaphor theory. By utilising the case of copyright in a digital context it explains the role that metaphor plays when the law is dealing with technological change, displaying both conceptual path-dependence as well as what is called non-legislaStefan Larsson's Conceptions in the Code contributes invaluably to socio-legal analysis and conceptual metaphor theory. By examining cases of digital copyright, Larsson elucidates the role that metaphor plays when law is accommodating technological change, displaying both conceptual path-dependence as well as non-legislative developments in law. Analyses draw from conceptual studies of property sp
INTRODUCTION: Merkel cell carcinoma is a rare malignant cutaneous neoplasm that is locally invasive and frequently metastasizes to lymph nodes, liver, lungs, bone and brain. The incidence of Merkel cell carcinoma has increased in the past three decades.CASE PRESENTATION: A 65-year-old Caucasian man presented with a sudden onset of severe headache and a three-month history of balance disturbance. M
We investigate suitable conditions for a C0-semigroup (T(t))t≥0 of Hilbert space contractions to be unitarily equivalent to the restriction of the adjoint shift semigroup (S∗γ(t))t≥0 to an invariant subspace of the standard weighted Bergman space Aγ−2(C+,K). It turns out that (T(t))t≥0 admits a model by (S∗γ(t))t≥0 if and only if its cogenerator is γ-hypercontractive and limt→0T(t)=0 in strong ope
