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Enzymatic hydrolysis of Canna indica, Manihot esculenta and Xanthosoma sagittifolium native starches below the gelatinization temperature

The susceptibility to enzymatic hydrolysis of starch extracted from raw roots of Canna indica, Manihot esculenta and Xanthosoma sagittifolium, grown in Bolivia, were studied below the gelatinization temperature. The granule size and amylose content were as followed: C. indica>M. esculenta>X. sagittifolium. The hydrolysis showed a rapid rate at the initial stage, followed by a progressive decrease

Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip.

Due to the extensive use of nucleic acid and protein analysis of bacterial samples, there is a need for simple and rapid extraction protocols for both plasmid DNA and RNA molecules as well as reporter proteins like the green fluorescent protein (GFP). In this report, an electropermeability technique has been developed which is based on exposing E. coli cells to low voltages to allow extraction of

A capacitive DNA sensor-based test for simple and sensitive analysis of antibiotic resistance in field setting.

To meet urgent needs for solving serious worldwide drug-resistance problems, a sensitive label-free capacitive sensor developed in our group was investigated as a tool to be applied in the field of antibiotic resistance genotyping, for instance the detection of ampicillin resistance gene (ampR). Proof-of-concept data demonstrated its detection sensitivity of pico-molar without any signal amplifica

Determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in human urine

A method for simultaneous determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in urine is described. These compounds are metabolites of N-methyl-2-pyrrolidone, a powerful and widely used organic solvent. 5-Hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide were purified from urine by adsorption to a C8 solid-phase extraction column and then elution by ethyl

Contraceptive use and reproductive history in women with cervical human papillomavirus infection

Abstract in French Cette étude a été réalisée pour déterminer si les papillomatoses virales du col sont associées à l'utilisation des contraceptifs et aux antécédents de reproduction. Après avoir enregistré les antécédents de contraception et de reproduction de 972 femmes ayant demandé des conseils en matière de contraception, des examens ont été conduits pour dépister toute infection de papillomaThe study was conducted to investigate whether cervical human papillomavirus infections (CHPI) are associated with contraceptive use and reproductive history. The contraceptive and reproductive histories in 972 women seeking contraceptive advice were noted and screening conducted for human papillomavirus infection. The interview included number of pregnancies and childbirths, legal and spontaneous

Långtidsöverlevnaden vid diabetes har successivt förbättrats. Lång sjukdomsduration kan ge information om protektiva möjligheter

Studies on patients with long-term diabetes survival without severe complications can give information about protective factors. Therefore, the present study aims to describe the long-term survival of patients with diabetes during successive periods following the introduction of insulin therapy in 1923. After registration in 1973 of the first local diabetic patient in Jonkoping with a fifty-year s

Conformations and Photophysics of a Stilbene Dimer.

Photophysical studies of pseudo-p-distyryl[2.2]paracyclophane, a model molecule of a stilbene dimer arranged in a brick-wall geometry, in tetrahydrofurane solution indicate the existence of at least two conformers in the ground state. The conformer A with the smallest optical gap of 3 eV has a short radiative lifetime of 3.3 ns, while the conformer B with an optical gap about 0.3 eV larger is much

Production of Xi(0)(c) and Xi(b) in Z decays and lifetime measurement of Xi(b)

The charmed strange baryon Xi(c)(0) was searched for in the decay channel Xi(c)(0) -> Xi(-)pi(+), and the beauty strange baryon Xi(b) in the inclusive channel Xi(b) -> Xi(-)l(-)(nu) over barX, using the 3.5 million hadronic Z events collected by the DELPHI experiment in the years 1992-1995. The Xi(-) was reconstructed through the decay AT, using a constrained fit method for cascade decays. An iter

Effects of sympathetic denervation on the hyaluronan content of the anterior segment in the normal and traumatized rabbit eye.

PURPOSE: To determine whether there is any involvement of sympathetic nerves in the regulation of ocular hyaluronan production in the normal and traumatized rabbit iris. METHODS: Unilateral sympathetic denervation was performed by removing the right superior cervical ganglion. Hyaluronan concentrations in the iris and aqueous were measured with a radiometric assay at various time intervals after d

Novel PPAR gamma agonists GI 262570, GW 7845, GW 1929, and pioglitazone decrease calcium channel function and myogenic tone in rat mesenteric arteries

Novel non-thiazolidinedione, tyrosine-derived peroxisome proliferator-activated receptor gamma agonists, GI 262570, GW 7845, GW 1929, developed by Glaxo-Smith-Kline (GSK) along with pioglitazone and nisoldipine, were studied on currents through L-type voltage-dependent calcium channels (VDCC) in freshly isolated smooth muscle cells from mesenteric arteries, and on the diameter of pressurized mesen

Direct evidence of active sympathetic vasodilatation in the skin of the human foot

1. During operative aorto-femoral vascular reconstructions on sixteen patients, the sympathetic chain was stimulated electrically between the L2 and L4 ganglia while blood flow was monitored by laser doppler flowmeters from the skin on the sole of the foot and the ankle and by an electromagnetic flowmeter from the deep femoral artery. Epidural anaesthesia to at least the T6 level was used which ex