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Nuclear magnetic resonance (NMR) spectroscopy was used in order to investigate the relationships between structure, dynamics and calcium binding in the intracellular regulatory protein, calmodulin. Calmodulin consists of two similar domains, each binding two calcium ions with positive cooperativity. The studies described in this thesis focus on the isolated C-terminal domain, TR2C, and two mutants

The effect of citric acid (CA) on starch films has been examined. A new method to detect cross-linking of starch by CA in solution-cast films by molecular weight measurements is described. Furthermore, we managed to distinguished between free, mono- and di-esterified CA and quantify di-ester content within starch films by using a modification in the method of complexometric titration with copper(I

Measurements of water content profiles are of great interest in hydrology and soil science. Time domain reflectometry (TDR) is a well-established method for water content measurements; however, most TDR probe designs are suitable for measurements in only a small soil volume. In this article, a 1-m long TDR profiling probe with five measurement sections is described. Unlike most other previous prof

BACKGROUND The nature and underlying mechanisms of an inverse association between adult height and the risk of coronary artery disease (CAD) are unclear. METHODS We used a genetic approach to investigate the association between height and CAD, using 180 height-associated genetic variants. We tested the association between a change in genetically determined height of 1 SD (6.5 cm) with the risk of

In this study, we show a significantly reduced assay time and a greatly increased bead recovery for a commercial Luminex-based multiplex diagnostic immunoassay by performing all liquid handling steps of the assay protocol in a non-contact acoustic trapping platform. The Luminex assay is designed for detecting antibodies in poultry serum for infectious bursal disease virus, infectious bronchitis vi

In a forest ecosystem at steady state, net carbon (C) assimilation by plants and C loss through soil and litter decomposition by heterotrophic organisms are balanced. However, a perturbation to the system, such as increased mean soil temperature, will lead to faster decay, enhancing CO2 release from decomposers, and thus upsetting the balance. Recent in situ experiments have indicated that the sti

Uptake of arsenic, molybdenum, uranium and vanadium by species of natural vegetation (Agrostis capillaris, Betula pendula, Calluna vulgaris, and Deschampsia flexuosa) on two contrasting, highly acid soils (pH of soil solution 4.2-4.3), differing in natural abundance of these elements, was compared. the soil developed from alum shale was rich in these elements, the soil from a gneiss moraine was po

The lasting effects of a recruitment maneuver (RM) in the injured lung are not well characterized. We speculated that the reduction in respiratory elastance (H) after a deep inflation (DI) is transient in nature and should be sustained longer at higher positive end-expiratory pressure (PEEP). Thirteen ventilated mice were given 2 DIs at various levels of PEEP before and after saline lavage. Forced

During drying, timber distortion is a major defect mainly due to shrinkage anisotropy, differences in longitudinal shrinkage, and spiral grain. The distortion can be reduced by external restraint and use of appropriate kiln schedules. The research presented here is part of a project on the improvement of shape stability of Norway spruce by high-temperature drying. The effects of drying temperature

A tissue proteomics process is presented where hepatocyte cell isolation in combination with two-dimensional (2-D) gel electrophoresis and mass spectrometric identification were used to annotate the liver proteome. Laser microdissection of 8 m liver tissue sections was performed and protein expression profiling was compared using a variety of quantities of input cells, and gel separation condition

A cohouse is a house with private apartments as well as shared areas and they can be organized in different ways. The purpose of the project was to learn more about cohousing. How the common rooms are used and what kind of furniture is preferred. My method was to read about the topic and then visit cohouses in Sweden and Germany to do interviews and study the use and function of the sha

The objective of this thesis was the impact evaluation of several parameters affecting daylight conditions in a typical cellular office in Lund, Sweden. The research approach that was followed consisted of three distinct steps; the literature review in order to specify the dependent and independent variables of the study, the simulation part to produce daylight performance data and the graphical a

Under åren 1965-1974 genomfördes en omfattande bostadsproduktion i Sverige som betecknas miljonprogrammet. Då byggdes en miljon bostäder runtom i landet. Idag finns behov av att renovera dessa bostäder. I samband med det kan energisparåtgärder genomföras för att minska energianvändningen. Detta kan uppfyllas genom att åtgärda otäta väggar och byta ut äldre fönster som medför oönskade värmeförlusteIn order to maintain a good indoor environment, air pollutants such as moisture need to be removed by the ventilation. Among the Swedish Public Health Agency's general advices for ventilation and indoor environment, 3 g/m3 is a guideline value for moisture excess during winter months to not pose a risk to human health. The indoor climate parameters moisture excess, relative humidity and air va

Syftet med denna uppsats är att undersöka de skatterättsliga förhållandena för näringsverksamheters avdrag för sponsring riktad till idrottssektorn. Sponsring inom näringsverksamhet är avdragsgill som en utgift för att förvärva och bibehålla inkomster. Uppsatsen tar hjälp av tre typfall för att illustrera tre olika situationer där sponsring är aktuell. Genom dessa typfall så utreder uppsatsen hur The purpose of this thesis is to investigate the tax regulation regarding expenses for sponsorship addressed to the sports sector. Sponsorship is deductible as an expense to acquire and maintain income. This thesis uses three fictional situations to illustrate three different situations where sponsoring is at hand. Through these fictional situations the thesis investigates how the Supreme Administ

Hydrogels are polymeric networks capable of retaining large amounts of water. They have high biocompatibility and have thus received great attention for various biomedical applications, including as wound dressing and drug delivery agents. They can be fabricated using several methods and from various polymers. In this project, we explored the enzymatic crosslinking of gelatine hydrogels using micr