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Assays detecting antigen (Ag)-specific T-cell responses in immune-mediated processes are increasingly employed to understand disease pathogenesis and "immune staging". The quantity and quality of starting peripheral blood mononuclear cell (PBMC) preparations are important factors in the performance of such assays. We therefore compared final PBMC yield and function by modifying parameters at the b

Objective: To investigate the association between human herpesviruses and multiple sclerosis ( MS), as well as between measles virus and MS. Methods: The authors identified prospectively collected serum samples from 73 MS cases and retrospective sera from 161 MS cases in two population-based serum bank registers. Analyses of IgG antibody responses in cases and matched referents were performed for

Conclusions. The results indicate that SOM of extreme duration, and maybe also the treatment of SOM, are risk factors for developing permanent hearing loss, both conductive and sensorineural. Objective. Fluctuating or persisting hearing loss of varying degrees is known to accompany secretory otitis media ( SOM). The aim of this study was to detect possible hearing sequelae in young adults who had

Allochthonous organic carbon in aquatic systems is metabolized by heterotrophic bacteria, with significant consequences for the biostructure and energy pathways of freshwater ecosystems. The degree to which allochthonous substrates support growth of bacteria is largely dependent on bacterial growth efficiency (BGE), i.e., bacterial production ( BP) per unit of assimilated carbon. Here we show how

Process development, optimization and robustness analysis for chromatographic separation are often entirely based on experimental work and generic knowledge. This paper describes a model-based approach that can be used to am process knowledge and assist in the robustness analysis of an ion-exchange chromatography step using a model-based approach. A kinetic dispersive model, where the steric mass

Herbivorous zooplankton may have a pronounced influence on pelagic microorganisms in Arctic freshwaters. We quantified experimentally the size-selective feeding of several zooplankton groups on pelagic microorganisms in high Arctic tundra systems. Our experiments and field study focused on dominant herbivores in Arctic freshwaters, including the cladoceran Dophnia, the copepod Diaptomus and the an

BACKGROUND: Methylation at CpG dinucleotides in genomic DNA is a fundamental epigenetic mechanism of gene expression control in vertebrates. Proteins with a methyl-CpG-binding domain (MBD) can bind to single methylated CpGs and most of them are involved in transcription control. So far, five vertebrate MBD proteins have been described as MBD family members: MBD1, MBD2, MBD3, MBD4 and MECP2. RESULT

Pt (0.1 wt.%) on Al2O3, TiO2, and MgO supports were used for the low temperature combustion of methanol in both the absence and the presence of ammonia. Without NH3 in the gas the performance for the complete combustion decreases in the order Pt/MgO greater than or equal to Pt/TiO2 much greater than Pt/Al2O3 comparing a fixed amount of Pt surface area. In the presence of ammonia strong deactivatio

Multipotent self-renewing hematopoietic stem cells (HSCs) are responsible for reconstitution of all blood cell lineages. Whereas growth stimulatory cytokines have been demonstrated to promote HSC self-renewal, the potential role of negative regulators remains elusive. Receptors for tumor necrosis factor (TNF) and Fas ligand have been implicated as regulators of steady-state hematopoiesis, and if o

OBJECTIVES: To replicate the relation between the CSF HVA:5-HIAA ratio and psychopathic traits previously reported in a pilot group of 22 perpetrators of violent crimes. METHODS: CSF monoamine metabolite concentrations in another 28 violent and sexual offenders, aged 45 or below, referred to pretrial forensic psychiatric investigation, were compared to features of psychopathy according to the Psyc

This study aims att developing a methodology for investigating the relationship between the fluent and disfluent productions of the Swedish conjunction ATT ‘that’ and the complexity of speech fragments following them. A study of the syntactic structure of the speech fragments following ATT and their relation to the pragmatic structure of the discourse, in particular the fragments’ role as regards

A simplification of the laminin nomenclature is presented. Laminins are multidomain heterotrimers composed of alpha, beta and gamma chains. Previously, laminin trimers were numbered with Arabic numerals in the order discovered, that is laminins-1 to -5. We introduce a new identification system for a trimer using three Arabic numerals, based on the alpha, beta and gamma chain numbers. For example,

Objective Obstructive sleep apnoea (OSA) confers a risk of hypertension and cardiovascular complications. Both the renin-angiotensin-aldosterone system and OSA are important determinants of blood pressure, but it is not fully known how they interact. The aim of this study was to explore the interaction between the angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and O

Reconstitution studies of a protein from domain fragments can furnish important insights into the distinctive role of particular domain interactions and how they affect biophysical properties important for function. Using isothermal titration calorimetry (ITC) and a number of spectroscopic and chromatographic tools, including CD, fluorescence and NMR spectroscopy, size-exclusion chromatography and

This thesis concerns nozzles and their impact on droplet formation and droplet flight in a continuous ink jet printer. Methods are suggested to characterise droplet formation and droplet flight stability (D.F.S.). A study about the influence of droplet flight stability on the quality of print out is presented. A method to assess the charging process for the continuous ink jet is described. The dev

The presence of polyamines in living cells is crucial for survival. Due to their high net charge at physiological pH, polyamines effectively charge neutralize the phosphodiester backbone of DNA in an interaction that also may protect the DNA from external damage. We here present a study illustrating the influence of spermidine and spermine on the platination reactions of the model oligonucleotides

Context: T-3 action in neurons is essential for brain development. Recent evidence indicates that monocarboxylate transporter 8 (MCT8) is important for neuronal T-3 uptake. Hemizygous mutations have been identified in the X-linked MCT8 gene in boys with severe psychomotor retardation and elevated serum T-3 levels. Objective: The objective of this study was to determine the functional consequences

Neutron deficient nuclei near Sn-100 have been produced by fragmentation of a 1 (.) A GeV Sn-112 beam. The fragments were separated, identified and stopped in a highly segmented silicon strip detector stack. This detector measured the total energy of emitted beta(+)-particles. gamma-radiation was measured with surrounding detectors. The half-lives for many nuclides have been determined for the fir

ydrophobically modified polymers (HM-P) typically behave as thickeners in a wide range of systems. The thickening effect in an aqueous solution of this kind of polymer depends on intermolecular hydrophobic associations and also on chain entanglements if the polymer concentration is significantly above the overlap concentration. In the present investigation a rather short end-capped polymer has bee