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The present work reports on the development of a bi-enzyme biosensor using diaphorase from Bacillus stearothermophilus, nicotinamide adenine dinucleotide (NAD(+))- and glutathione (GSH)-dependent formaldehyde dehydrogenase (FDH) from the genetically-engineered methylotrophic yeast Hansenula polymorpha as bio-recognition elements. The sensor architecture comprises a first layer containing diaphoras

The ability to compare soil chemical data achieved by different chemical extraction methods is a necessity for an efficient long-term monitoring of soils and for comparisons of results from regional soil surveys with differing standard methods. This study compares two common, methods for analysis of soil chemical properties, the combination of soil extraction in 1.0 M NH4Cl and 1.0 M KCl and the s

The lack of a conserved tyrosine autophosphorylation site is a unique feature of the C-terminal Src-kinase, Csk, although this protein tyrosine kinase can be autophosphorylated on tyrosine residues in vitro and in bacteria. Here we show that human Csk is tyrosine phosphorylated in HeLa cells treated with sodium pervanadate. Phosphorylation in vivo occurs mainly at Tyr-184 and in vitro mainly at Ty

Sedan våren 2005 har en grupp tonåriga pojkar inom habiliteringen i Region Skåne engagerats i en till synes udda fritidsaktivitet. Tillsammans med medlemmar i en motorförening och en fritidsförening samlas de en kväll i veckan på en motorgård i Väla utanför Helsingborg för att meka med en gammal bil. ”Projekt Raggarbil” har på kort tid blivit mycket populärt bland ungdomarna, deras föräldrar och

Epitopes found on Mucin-1 are differentially expressed on tumour versus normal tissue. Such epitopes have also been shown to have a potential in immunotherapy and tumour detection. The major epitope explored in this context is located within the variable number of tandem repeats. It has however recently been demonstrated that this epitope exists in several sequence variants. The standard sequence

Purpose. To determine the potential application of high-intensity focused ultrasound for the minimally invasive treatment of herniated intervertebral discs by developing a probe that produces sufficiently high temperature locally to shrink collagen fibers (65-75 degrees C). Materials and Methods. A 5-mm ultrasound probe was produced with a geometric focal length of 15 mm. The probe produced 2.5 W

Objective. According to epidemiological studies, smoking habit is strongly associated with inflammatory bowel disease. Non-smokers, and especially recent ex-smokers, have an increased risk of ulcerative colitis (UC). Conversely, concerning Crohn's disease, the risk is increased among smokers. Pouchitis is the major long-term complication of restorative proctocolectomy for UC, and seems to be patho

A chemiluminescence inummoassay for the detection of four heart marker proteins: myoglobin, creatine kinase mb [CKmb], troponin I [Tnl], and fatty acid-binding protein [FABP], was designed. The inummoassay was based on enzyme-linked immunosorbent assay [ELISA] and antibodies immobilized in glass capillaries pre-treated with 3-aminopropyltriethoxysilane. The protein bound to the antibody was detect

Cross-sectional studies have reported strong correlations between plasma levels of complement C3, insulin, and glucose. This prospective study explored whether elevated levels of C3, C4, and other inflammation-sensitive plasma proteins (ISPs; fibrinogen, orosomucoid, α1-antitrypsin, haptoglobin, and ceruloplasmin) are associated with the development of diabetes. Plasma proteins were measured in 2,

We consider the Euler approximation of stochastic differential equations (SDEs) driven by Levy processes in the case where we cannot simulate the increments of the driving process exactly. In some cases, where the driving process Y is a subordinated stable process, i.e., Y = Z(V) with V a subordinator and Z a stable process, we propose an approximation Y by Z(V-n) where V-n is an approximation of

OBJECTIVE: New treatments have improved the prognosis for patients with acute myocardial infarction. However, studies on long-term survival are not unequivocally in favour of an improved long-term prognosis. This study aimed to analyse trends in 3-year survival in relation to sex, age and socioeconomic level of residential area. SETTING: The Malmo myocardial infarction register, Sweden. PARTICIPAN

ABSTRACT. Differences in labour force participation and unemployment rates between indigenous populations and immigrants are common throughout Europe. But the gap seems to be particularly wide in Sweden. Based on studies of work places that traditionally employed large numbers of immigrants, but where they are now declining, it is argued that a driving force behind this process of exclusion is to

The use of monoclonal antibodies (MAbs) in cytotoxic conjugates (radionuclides, toxins, or drugs) for targeting tumor cells is restricted due to toxicity in vital organs. Through improved tumor targeting, it is possible to administer larger amounts of such labeled MAbs, thus improving the ability to eradicate tumor cells without increased normal organ toxicity. Extracorporeal affinity adsorption t

Randomized, double-blind, controlled studies have shown that treatment with risedronate reduces the risk of vertebral fracture in postmenopausal women with established vertebral osteoporosis. They also show that the drug decreases the risk of non-vertebral fractures in women with osteoporosis. The aim of this study was to investigate the cost-effectiveness of risedronate in postmenopausal women wi

A general method is presented for patient-specific three-dimensional (3D) absorbed dose calculations based on quantitative SPECT activity measurements. The computational scheme includes a method for registration of the CT study to the SPECT image, and compensation for attenuation, scatter, and collimator-detector response including septal penetration, performed as part of an iterative reconstructi

Organophosphate hydrolase containing hexahistidine tag at the N-terminus of recombinant protein (His(6)-OPH) and expressed in Escherichia coli cells was purified using supermacroporous polyacrylamide-based monolith columns with immobilized metal affinity matrices [Me2+-iminodiacetic acid (IDA)-polyacrylamide cryogel (PAA) and Me2+-N,N,N'-tris (carboxymethyl) ethylendiamine (TED)-PAA]. Enzyme prepa

We present an abundance analysis of the very metal-poor, carbon-enhancedstar CS 29497-030. Our results indicate that this unusually hot turnoffstar (T_eff = 6650 K, log g = 3.5) has a metallicity [Fe/H] =-2.8, and exhibits large overabundances of carbon ([C/Fe] = +2.38),nitrogen ([N/Fe] = +1.88), and oxygen ([O/Fe] = +1.67). This star alsoexhibits a large enhancement in its neutron-captu