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High-throughput proteomics using antibody microarrays

Antibody-based microarrays are a novel technology that hold great promise in proteomics. Microarrays can be printed with thousands of recombinant antibodies carrying the desired specificities, the biologic sample (e.g., an entire proteome) and any specifically bound analytes detected. The microarray patterns that are generated can then be converted into proteomic maps, or molecular fingerprints, r

Soluble pilin of Neisseria gonorrhoeae interacts with human target cells and tissue

Pili of Neisseria gonorrhoeae are phase-variable surface structures that mediate adherence to host target cells. Each pilus is composed of thousands of major pilus subunits, pilins, pilus-associated protein PilC, and possibly other components. Piliated and nonpiliated gonococcal clones may secrete a soluble smaller pilin (S-pilin) that is cleaved after amino acid 39 of the mature pilin protein. He

Challenging the set mining path : Agency and diversification in the case of Kiruna

The paper follows the development of the tourism industry in a small, traditional mining town in northern Sweden. It highlights local agency in the diversification process, as well as interpath relations between tourism and mining. Drawing on 21 semi-structured interviews, the paper finds that the two seemingly unrelated paths share the need for a variety of local endowments and in so the dominant

Introduction : Candidates between parties and voters-A triadic relationship in the belgian partitocracy

The introductory chapter emphasises the general relevance of the study of electoral candidates in representative democracies. It presents the book’s theoretical framework: the triadic delegation and accountability relationship between candidates, voters and parties—based on principal–agent theory. We theoretically map patterns of candidates’ behaviour and attitudes in all main dimensions of their

Reading the Room : Messy Contradictions in the Datafied Home

In this chapter, I draw on the artwork LAUREN by Lauren Lee McCarthy and the theoretical ideas of algorithmic intimacies and datafication to reveal the messy contradictions of the datafied home. What I term as the concept of algorithmic intimacies is a way to think anew about the intimate relations between subjects, algorithms, and proximities in data.¹ As the analysis aims to surface, these messy

Wastewater treatment and reuse through natural treatment systems

Two successful samples of land treatment systems are introduced in the paper. A rapid infiltration system of the area 11.3 hectares with treatment capacity of 10,000 m3/day was constructed in Pingdu city of Shandong province. The removal of COD reached 87.5% while 89.4 for suspended solids and 64.4 % for NH3-N. In fact, the treated water already achieved the criteria of the secondary treatment eve

Comparison between comparative tuberculin and gamma-interferon tests for the diagnosis of bovine tuberculosis in Ethiopia

A study to determine and compare the sensitivities and specificities of the comparative cervical tuberculin (CCT) and gamma-interferon (IFN-gamma) tests for the diagnosis of bovine tuberculosis was conducted on 30 zebu oxen. The results of the tests were compared with the presence of acid-fast bacilli found by bacteriological culturing and histopathological examinations. The sensitivity and specif

Isolation of obligate anaerobic rumen bacteria capable of degrading the neurotoxin beta-ODAP (beta-N-oxalyl-L-alpha,beta-diaminopropionic acid) as evaluated by a liquid chromatography/biosensor analysis system

Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates wa

Motstånd och medarbetarinflytande vid förändringsarbete i offentlig sektor

Motstånd är ett vanligt problem vid förändringsarbete. I detta konferenspapper tolkas motstånd som en begäran om inflytande i ledningens beslutsfattande med avseende på förändringen. Här väljer ledningen en strategi – mer eller mindre medarbetarinflytande. I offentlig verksamhet måste denna ledningsstrategi kunna legitimeras mot principaler och samhälle. Det är därför särskilt viktigt att förstå h

antibody microarray analysis of directly labelled proteomes

In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols