Search results

The recently identified phylogenetic subgroup B5 of fungal glycoside hydrolase family 18 genes encodes enzymes with mannosyl glycoprotein endo-N-acetyl-b-D-glucosaminidase (ENGase)-type activity. Intracellular ENGase activity is associated with the endoplasmic reticulum associated protein degradation pathway (ERAD) of misfolded glycoproteins, although the biological relevance in filamentous fungi

A series of QM/MM optimizations of the full protein of [Fe] hydrogenase were performed. The FeGP cofactor has been optimized in the water-bound resting state (1), with a side-on bound dihydrogen (2), or as a hydride intermediate (3). For inclusion of H4MPT in the closed structure, advanced multiscale modeling appears to be necessary, especially to obtain reliable distances between CH-H4MPT+ and th

Severe appetite and weight loss define the eating disorder anorexia nervosa, and can also accompany the progression of some neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS). Although acute loss of hypothalamic neurons that produce appetite-stimulating neuropeptide Y (Npy) and agouti-related peptide (Agrp) in adult mice or in mice homozygous for the anorexia (anx) mutation ca

The thiol activity of pancreatic islets was spectrophotometrically assayed as the formation of 6-mercaptonicotinic acid from the organic disulfide, 6, 6’-dithiodinicotinic acid. Islets containing more than 90% β-cells were microdissected from non-inbred oblob-mice. Comparisons of intact with homogenized islets indicated that the organic disulfide penetrates relatively slowly into the β-cells. When

Bacterial ice-nucleating particles (INP) are present in the atmosphere and efficient in heterogeneous ice-nucleation at temperatures up to -2 °C in mixed-phase clouds. However, due to their low emission rates, their climatic impact was considered insignificant in previous modeling studies. In view of uncertainties about the actual atmospheric emission rates and concentrations of bacterial INP, it

Prospective observational studies have shown previously that study participants have lower morbidity and mortality than non-participants. The aim of the current study was to determine whether participants in a prospective cohort study on melanoma have a different incidence and mortality of melanoma compared with non-participants and the background population. Information was collected from Swedish

Background: IRL752 is a novel small-molecule compound that acts to regioselectively enhance norepinephrine, dopamine, and acetylcholine neurotransmission in the cerebral cortex. Objective: The primary objective of the trial was to investigate the safety and tolerability of IRL752 in patients with Parkinson's disease and dementia. Methods: Patients with Parkinson's disease and dementia were randomi

The rooms in Intensive Care Units are considered as high-tech environments and believed to affect recovery process and wellbeing of patients. Moreover, the design and interiors affect the interplay between the patient and the next of kin. Objective: The aim of this study was to describe and interpret the meanings of the intensive care patient room as experienced by next of kin. Design: Next of kin

High sound levels related to medical equipment and high activity day and night, is a wellknown phenomenon in intensive care units (ICUs). This may result in poor sleep and prolonged recovery for the patients but may also be a precipitating factor for intensive care delirium (ICU delirium).The aim of this pilot study was to describe patients' responses to the sound environment in an ICU and to iden

This article explores the potential of combining high‐performance computing techniques and a set of integrated digital methods to investigate the cityscape of ancient Hermione, Greece. Unmanned aerial vehicles (UAVs), terrestrial laser scanning, image‐based modelling techniques and high‐performance computing have been combined to provide a fully‐three‐dimensional (3D) representation of the city la

ABSTRACT In field‐trapping experiments the unsaturated analogues (E)‐6‐, (E)‐12‐, and (Z)‐12‐tetradecenyl acetate were as attractive to male Yponomeuta rorellus Latr. as the native pheromone component tetradecyl acetate. All four analogues attracted more males than virgin females did, whereas (Z)‐6‐, (E)‐11‐, (Z)‐10‐ and (Z)‐11‐tetradecenyl acetate were essentially non‐attractive. Addition of 1–30

BACKGROUND AND AIMS: Genetic variants involved in vitamin D metabolism have been associated with diabetes and related syndromes/diseases. We wanted to investigate possible associations of polymorphisms in genes involved in vitamin D metabolism with indices of insulin resistance and insulin secretion, and also with development of diabetes after gestational diabetes mellitus (GDM).MATERIALS AND METH

The Klotho (KL) gene is involved in phosphate homeostasis. Polymorphisms in this gene have been reported to be associated with the risk of cardiovascular disease. Here we used computational tools to predict the damage-associated single nucleotide polymorphisms (SNPs) in the human KL gene. We further investigated the association of SNPs in the KL gene and mortality in the Swedish multicenter prospe

In contrast to all known deoxyribonucleoside kinases, a single highly efficient deoxyribonucleoside kinase from Drosophila melanogaster (Dm-dNK) is able to phosphorylate all precursor nucleosides for DNA synthesis. Dm-dNK was mutated in vitro by high-frequency random mutagenesis, expressed in the thymidine kinase-deficient Escherichia coli strain KY895 and clones were selected for sensitivity to t

Women gain less advantage than men in the history of neoliberalism. In this thesis, the author reviews the influence of neoliberal globalization on local women in the comparison of Swedish and Chinese context and discovers their different ways of doing gender. In order to examine the impact of neoliberal capitalism and globalization upon Chinese indigenous women, the author reviews a Mosuo documen

This is a comparison between the different loves expressed in the two novels. The loves are those expressed between the woman and the two men, in the Twilight Saga it is the love between the girl and the human on one side and the other it is the love between the girl and the werewolf and the loves in that book is compared to the loves in Jane Eyre, the one between Jane and her true love Rochester