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The growing field of biotechnology is in constant need of binding proteins with novel properties. Not just binding specificities and affinities but also structural stability and productivity are important characteristics for the purpose of large-scale applications. In order to find such molecules, libraries are created by diversifying naturally occurring binding proteins, which in those cases serv
The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples within
The solution properties of two water-soluble polymers, poly(acrylic acid) (PAA), covalently labeled with the fluorescent hydrophobic dye naphthalene (Np), have been investigated in water:organic solvent mixtures. The naphthalene chromophores have been randomly attached, onto the polymer, with two different degrees of labeling. Fluorescence measurements (steady-state and time-resolved) have been us
To assess the manifestation and location of airway inflammation in smokers with chronic bronchitis (CB) or chronic obstructive pulmonary disease (COPD), we lavaged the airways of 12 smokers with CB and 11 smokers with COPD and coexisting CB (OCB). For comparison, the airways of 5 asymptomatic smokers (AS) and 10 healthy nonsmokers (HNS) were lavaged. In all cases, the first lavage aliquot, labeled
This essay examines the different views on the inauguration of ”baby boxes” in the Polish society. The public debate in Poland has long been infused by catholic rethorics. The setting-up of ”baby boxes” in the Polish society has been warmly welcomed by conservatives in the country. The catholic church has taken on the responsibilty for the ”baby boxes”, and today most of the operating boxes in the
The particularities surrounding the way decentralized multinational companies are structured and governed can determine the success or failure of integrating sustainability aspects into their units’ approach of doing business. The choice of pursuing a decentralized avenue over a headquarter-driven one in managing sustainability can have a significant influence over the coherence of Group1-wide str
A substantial part of the population on the African continent suffers massive human rights crimes. Torture, murder, enslavement and systematic rape of civilians occur on a daily basis in several African countries. The international trade of natural resources from Africa, such as diamonds and other minerals, finances violence and immense human rights violations. The purpose of this study is to exa
According to international law, under the Convention Relating to the Status of Refugees (CRSR), refugees have the right to seek asylum in the host country they arrive to. However, there are many democratic states today that view it as their privilege to select which refugees who have the right to enter their territory. In order to do research on this subject I will use Norman Fairclough’s critical
It is a hotly debated issue whether second language (L2) learners can achieve target-like online syntactic processing of relations not instantiated in their first language (L1). The Shallow Structure Hypothesis (SSH) predicts that L2 learners will not process configurations with structural distance in a target-like online fashion (Clahsen & Felser, 2006 a,b,c), whereas some studies find that t
Topology optimization is used to design phononic bandgap materials that are tunable by mechanical deformation. A periodic media is considered, which due to the assumption of length scale separation, allows the dispersion relations to be obtained by analyzing a single unit cell subjected to Floquet–Bloch boundary conditions. A finite macroscopic deformation is applied to the unit cell to affect its
Proteomics Data Collection (ProDaC) is an EU-funded Coordination Action within the 6th framework programme. It aims to simplify the publication, dissemination and utilization of proteomics data by establishing standards that will support broad data collection from the research community. As a part of ProDaC, regular workshops are organized on a half-yearly basis to enable communication and discuss
Purpose: An elevated sense of vocal effort due to increased vocal demand is frequently reported by patients with voice disorders. However, effects of vocal warm-up on self-assessed vocal effort have not been thoroughly examined. A recently developed version of the Borg CR-10 Scale facilitates vocal effort assessments, following different vocal warm-up tasks. Methods: Effects of a short (5 min) voc
The Secret Diaries of Miss Anne Lister (2010) and Gentleman Jack (2019–2022) are both television dramas based on the private journals of Anne Lister (1791–1840). The diaries were not only partly written in code but make use of idiosyncratic terms, and the two adaptations deal in different ways with the language used in the diaries. This essay examines the language of Anne Lister’s diaries and com
Visual information can be integrated in prominence perception, but most available evidence stems from controlled experimental settings, often involving synthetic stimuli. The present study provides evidence from spontaneously produced head gestures that occurred in Swedish television news readings. Sixteen short clips (containing 218 words in total) were rated for word prominence by 85 adult volun
During eyeblink conditioning, Purkinje cells that control blinking develop an inhibitory response to the conditional stimulus. This conditional response (CR) in the Purkinje cell shows a number of striking similarities to the overt blink CR, including adaptive timing. The evidence suggests that the Purkinje cell CR drives the overt CR. Recent results show that the Purkinje cell CR does not depend
