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The feasibility and scalability of extraction in detergent-based aqueous two-phase systems for the separation of proteins from culture broth is demonstrated. At the same time the large-scale production of a fusion protein and the influence of cultivation scale on the efficiency of separation were investigated. An amphiphilic fusion protein EGIcore-HFBI was chosen, consisting of the catalytic core
Phonological vowel reduction in Bulgarian is discussed, distinguishing between formal & informal speech & dialectal & standard forms. The Bulgarian vowel system is presented, with an evaluation of the articulatory models of Bulgarian vowel production, & a description of the orthographic representation of the "indeterminate vowel." Alternations between stressed & nonstressed vow
Previous studies have shown increased cardiovascular mortality as late side effects in Hodgkin lymphoma (HL) patients. This study identifies stratifying risk factors for surveillance and defines concepts for a clinical feasible and noninvasive prospective protocol for intervention of cardiovascular side effects. HL patients diagnosed between 1965 and 1995 (n = 6.946) and their first-degree relativ
The fire technical properties of products are determining factors for the initiation and growth of fires. Measurement of fire technical properties and the understanding of how they relate to real hazards are therefore important for fire safety. This work deals with the fire technical properties of building products, their tendency to ignite and release heat in different fire scenarios. Building p
2,8-Dihalo substituted analogues of Tröger’s base were prepared via the Tröger’s base condensation reaction. These compounds were used as synthetic intermediates for the preparation of otherwise inaccessible functionalized Tröger’s base analogues. Based on 2,8-diiodo-4,10-dimethyl-6H,12H-5,11-methanodibenzo[b,f][1,5]diazocine, a mild room temperature protocol for the introduction of terminal func
Popular Abstract in Swedish Den pågående ökningen av växthusgaser i atmosfären orsakar en ökning av den globala medeltemperaturen. En viktig påverkande faktor för koncentrationen koldioxid (CO2) i atmosfären är utbytet mellan atmosfären och biosfären; fotosyntesen tar upp CO2 och respirationen släpper ut CO2, vilket resulterar i ett nettoutbyte. För att öka vår förståelse av den spatiala och tempoThe ongoing increase in atmospheric greenhouse gas concentrations causes an increase in the global mean air temperature. One important moderator of the atmospheric carbon dioxide (CO2) concentration is the gas exchange between the atmosphere and the biosphere; photosynthesis extracts and respiration releases CO2, resulting in a net exchange. A variety of methods are used to improve our understandi
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This paper describes the participatory process of developing and implementing a prototype model aimed at supporting the Integrated Sustainability Assessment of water resources and policy options at different scales. The model - called the World Cellular Model (WCM) focuses on the representation of agents’ behaviours and their systemic relationships with their environment. This is achieved by exami
This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. Automated closed-tube quantitative real-time PCR favours the analysis of food-borne pathogenic bacteria. However, in common with conve
GHRS observations of chemically peculiar stars have initiated a new project for calculating atomic properties of heavy elements (Z approximate to 80). The method used is based on large-scale, fully relativistic MCDF, and the data involve accurate gf-values, hyperfine structure parameters, and relative isotope shifts.
As climate models can be used to reproduce historical climates, the outcomes can be used to put climate extremes in to a proper historical perspective. This also allows investigation of nonlinear properties of hydrologic processes (e. g. precipitation, runoff) to better understand regional hydrologic dynamics. To this end, the present study uses results from a so-called 'paleosimulation' (i.e. sim
It has been recently reported that both dynamic movement characteristics, as well as the duration of postures adopted during work, are important in the development of low back pain (LBP). This paper presents a video-based posture assessment method capable of measuring trunk angles and angular velocities in industrial workplaces. The interobserver reliability, system accuracy, and the relationship
With the increasing output power of the integrated circuit chips, the heat flux involved is being accordingly increased. In such situation, the air has almost reached its limit of cooling capacity, and thus the liquid cooling technology incorporating microchannel heat sinks is desired to cool the electronic chips in order to remove more heat loads. However, these microchannel heat sinks are often
Up-regulation of G-protein-coupled receptors (GPCR) plays key roles in renal hypertension and cardiovascular disease pathogenesis. The present study was designed to examine if lipid-soluble cigarette smoking particles (DSP), nicotine and endotoxin (LPS), induce GPCR up-regulation for thromboxane A(2) (TP), endothelin type A (ETA) and type B (ETB) receptors in renal artery, and if intracellular sig
