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Engineering of pyranose 2-oxidase: Improvement for biofuel cell and food applications through semi-rational protein design

Pyranose 2-oxidase (P20x) has several proposed biotechnological applications such as a bio-component in biofuel cells or for carbohydrate transformations. To improve some of the catalytic properties of P20x from Trametes multicolor, we selected a semi-rational approach of enzyme engineering, saturation mutagenesis of active-site residues and subsequent screening of mutant libraries for improved ac

Impact of site-specific conjugation strategies on the pharmacokinetics of antibody conjugated radiotherapeutics

Antibody radionuclide conjugates are an emerging modality for targeted imaging and potent therapy of disseminated disease. Coupling of radionuclides to monoclonal antibodies (mAbs) is typically achieved by applying non-site-specific labelling techniques. With the ambition of reducing variability, increasing labelling efficacy and stability, several site-specific conjugation strategies have been de

A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity

Accurate detection of SARS-CoV-2 neutralizing antibody (nAb) is critical for assessing the immunity levels after virus infection or vaccination. As fast, cost-effective alternatives to viral infection-based assays, competitive binding (CB) assays were developed to quantitate nAb by monitoring the ability of sera to inhibit the binding of viral spike (S) protein to the angiotensin converting enzyme

Heat transfer through protective clothing under symmetric and asymmetric long wave thermal radiation

Abstract in French Cette etude reprend les resultats d'une recherche co-financee par la Communaute Europeenne sur les effets thermiques des vetements de protection et sur l'astreinte physiologique qui en resulte dans les conditions de travail. L'etude concerne l'infiuence de l'asymetrie de rayonnement thermique. L'echange thermique a ete mesure al'aide d'un mannequin calorimetrique sous l'influencThis report considers results of an ED funded research on thermal properties ofprotective clothing and their use in the assessment of the thermal strain at work. In order to study the effects of the asymmetry oflong wave thermal radiation on the heat transfer through protective clothing, the heat loss under all-side and unilaterally applied radiation with the same incident radiant power of 279 W/m

Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5)

Action Plan B3 of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) focuses on the integrated care of chronic diseases. Area 5 (Care Pathways) was initiated using chronic respiratory diseases as a model. The chronic respiratory disease action plan includes (1) AIRWAYS integrated care pathways (ICPs), (2) the joint initiative between the Reference site MACVIA-LR (Contre