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Selection of random RNA fragments as method for searching for a site of regulation of translation of E. coli streptomycin mRNA by ribosomal protein S7

In E. coli cells ribosomal small subunit biogenesis is regulated by RNA-protein interactions involving protein S7. S7 initiates the subunit assembly interacting with 16S rRNA. During shift-down of rRNA synthesis level, free S7 inhibits self-translation by interacting with 96 nucleotides long specific region of streptomycin (str) mRNA between cistrons S12 and S7 (intercistron). Many bacteria do not

On exposure to anorexia nervosa, the temporal variation in axial and appendicular skeletal development predisposes to site-specific deficits in bone size and density : A cross-sectional study

Skeletal development is heterogeneous. Throughout growth, bone size is more maturationally advanced than the mineral being accrued within its periosteal envelope; before puberty, appendicular growth is more rapid than axial growth; during puberty, appendicular growth slows and axial growth accelerates. We studied women with differing age of onset of anorexia nervosa to determine whether this tempo

Assessing data‐scarce contaminated groundwater sites surrounding petrochemical industries

A common problem when studying groundwater contamination in low-income countries is that data required for a detailed risk assessment are limited. This study presents a method for assessment of the potential impact of groundwater contamination by total petroleum hydrocarbons (TPH) in a data-scarce region. Groundwater modeling, using the MODFLOW, was used to simulate regional-scale flow pattern. Th

The application of resistivity and IP-measurements as investigation tools at contaminated sites : a case study from Kv. Renen 13, Varberg, SW Sweden

Kv. Renen 13 i centrala Varberg är sedan tidigare känt som kraftigt förorenat. På fastigheten har det från slutet av 1800-talet fram till 2003 funnits verksamheter i form av textilindustri som avlösts av finmekanisk verkstadsindustri. Det är framförallt den sistnämnda verksamheten som har orsakat rådande situation med höga halter klorerade lösningsmedel, trikloreten (TCE) och 1,1,1-trikoletan (TCAAn old industry estate, Kv. Renen 13, is located in central Varberg, southwest Sweden, and is known from previous investigations as highly polluted. Pollution started in the late 19th century with textile manufacturing, and in the 1960´s changed into a precision mechanics industry using chlorinated solvents, primary trichloroethene (TCE) and 1,1,1-trichloroethane (TCA), as well as cyanide and meta

Phosphorylation sites of Arabidopsis MAP kinase substrate 1 (MKS 1)

The Arabidopsis MAP kinase 4 (MPK4) substrate MKS1 was expressed in Escherichia coli and purified, full-length, 6x histidine (His)-tagged MKS1 was phosphorylated in vitro by hemagglutinin (HA)-tagged MPK4 immuno-precipitated from plants. MKS1 phosphorylation was initially verified by electrophoresis and gel-staining with ProQ Diamond and the protein was digested by either trypsin or chymotrypsin f