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Purification and identification of the violaxanthin de-epoxidase as a 43 kDa protein

Violaxanthin deepoxidase (VDE) has been purified from spinach (Spinacia oleracea) leaves. The purification included differential sonication of thylakoid membranes, differential (NH4)2SO4 fractionation, gel filtration chromatography and finally either hydrophobic interaction chromatography or anion exchange chromatography. A total purification of more than 5000-fold compared to the original thylako

Poison, bad hearts and vampires: the fear of contamination and the regulation of social relationships in a Rio de Janeiro favela

This article analyzes instances of refusal to eat or drink due to the fear of poisoning and contamination amongst favela youth in Brazil. These particular occurrences of refusal shed light on refusal’s potency as a way of managing complex social entanglements and instrumental in asserting agency. Considering that interlocutors had come of age without many formal institutions present, they needed p

Virtual participatory mapping of nature-based solutions in the Grande de Tárcoles River basin, Costa Rica : Connecting diverse knowledge systems in a context of physical immobility

Co-design processes are of key importance in planning and implementing Nature-based Solutions (NBS), but require boundary management between diverse holders of local, practical and scientific knowledge. Participatory and mapbased planning tools, such as Geodesign workshops, can facilitate such boundary management through mobilising knowledge holders, and translating and negotiating between differe