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A tandem mass spectrometric approach to determination of chondroitin/dermatan sulfate oligosaccharide glycoforms

Dermatan sulfate (DS) chains are variants of chondroitin sulfate (CS) that are expressed in mammalian extracellular matrices and are particularly prevalent in skin. DS has been implicated in varied biological processes including wound repair, infection, cardiovascular disease, tumorigenesis, and fibrosis. The biological activities of DS have been attributed to its high content of IdoA(alpha 1-3)Ga

Putative xylose and arabinose reductases in Saccharomyces cerevisiae

Saccharomyces cerevisiae mutants, in which open reading frames (ORFs) displaying similarity to the aldo-keto reductase GRE3 gene have been deleted, were investigated regarding their ability to utilize xylose and arabinose. Reduced xylitol formation from D-xylose in gre3 mutants of S. cerevisiae suggests that Gre3p is the major D-xylose-reducing enzyme in S. cerevisiae. Cell extracts from the gre3

Characterization of Chemical Exchange Using Residual Dipolar Coupling

Abstract in Undetermined NMR line shape analysis and relaxation dispersion measurements for N,N-dimethyltrichloroacetamide (DMTCA) weakly aligned using poly-γ-benzyl-l-glutamate, which forms a lyotropic nematic phase when dissolved in chloroform, were used to characterize chemical exchange kinetics for the rotation around the C−N amide bond. At low temperatures (312 K), a single population-average

Multilayered metal core-shell nanostructures for inducing a large and tunable local optical field

We examine the near-field optical properties of multilayered metal core-shell sphere nanostructures by extending the Mie theory, and demonstrate that the local-field enhancement can be increased by adding new metal shells. A local intensity enhancement of more than 10(5) times can be obtained in the cavity between the Ag core and the innermost Ag shell for proper configurations of multilayered Ag

Introduktion till konsekvensberäkningar. Några förenklade typfall

I dagens samhälle krävs i många fall genomförande av risk- och/eller konsekvensanalyser. Krav ställs dels från myndigheter och dels inom enskilda företag och organisationer. Här kommer endast konsekvensanalyser att behandlas. Anledningen till att genomföra en konsekvensanalys kan skifta. Som nämnts kan krav på en konsekvensalanys komma utifrån från myndigheter och dels kan en analys utföras för at

Role and mechanisms of secondary mitochondrial failure

Ischemia is accompanied by mitochondrial dysfunction, as assessed by measurements of mitochondrial respiratory activities in vitro. Following brief periods of ischemia, mitochondrial function is usually normalized during reperfusion. However, particularly after ischemia of longer duration, reperfusion may be accompanied by secondary mitochondrial failure. After short periods of ischemia this is ob

The GDP-GTP exchange factor collybistin: An essential determinant of neuronal gephyrin clustering

Glycine receptors (GlyRs) and specific subtypes of GABA(A) receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain

Different genotypes causing indiscernible patterns of A expression on A(el) red blood cells as visualized by scanning immunogold electron microscopy

BACKGROUND AND OBJECTIVES: The published sequence of the weak. A subgroup Ael gene from Swedish individuals showed a G insertion in exon VII, causing a frameshift at codon 268 (the A1 gene has 353 codons). We wished to sequence exons VI and VII of two Norwegian Ael individuals and compare the expression of A substance on RBC from different Ael individuals. MATERIALS AND METHODS: Exon VI and VII we

Fire alarm in a public building: How do people evaluate information and choose evacuation exit?

Personers reaktioner har studerats genom experimentella studier samt i genomgång av litteraturen. I denna undersökning har valet av utrymningsväg vid en simulerad brand studerats utifrån olika förutsättningar vad avser avstånd till utrymningsvägen och om dörren var öppen eller stängd. En andra del i försöket studerade hur personer reagerade och uppfattade situationen om en liten brand befann sig mHow people react on fire alarm have been studied in retrospect as well as in experiments. In the present study, the choice of exit was examined with respect to the distance to exits and open or closed emergency exit. The second part covers the question on how the subjects think and react in a situation having a small fire in the escape route. The third part deals with some communication aspects re

Women with Primary Sjögren´s Syndrome Assessment and Treatment A Physiotherapeutic Perspective

Popular Abstract in Swedish Primärt Sjögrens syndrom är en reumatisk systemsjukdom, som karaktäriseras av nedsatt funktion i vissa körtlar, framför allt i tårkörtlar och spottkörtlar. Detta orsakar huvudsymptomen ögon- och muntorrhet. Patienter med denna sjukdom har även besvär som inte är relaterade till dessa körtlar. Smärta i leder och muskler, extrem trötthet samt nedstämdhet är vanliga problePrimary Sjögren´s syndrome (primary SS) is a systemic autoimmune rheumatic disease characterized by lymphocytic infiltrations in the exocrine glands leading to classic dryness complaints such as dry eyes and dry mouth. Non-exocrine complaints also occur, e.g. fatigue, myalgia and arthralgia, as well as disturbed mood. The overall purpose of this work was, from a physiotherapeutic perspective, to i

Coeliac disease is associated with intrauterine growth and neonatal infections

To investigate whether factors in the fetal or neonatal period influence the risk of later development of coeliac disease we conducted a population-based register study. The Swedish Medical Birth Register was linked with the Hospital Discharge Register and identified 3392 singleton infants born in the period 1987-97 who developed coeliac disease. Perinatal data for these children were compared wit

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation and comparison to the WOMAC in total knee replacement.

BACKGROUND: The Knee injury and Osteoarthritis Outcome Score (KOOS) is an extension of the Western Ontario and McMaster Universities Osteoarthrtis Index (WOMAC), the most commonly used outcome instrument for assessment of patient-relevant treatment effects in osteoarthritis. KOOS was developed for younger and/or more active patients with knee injury and knee osteoarthritis and has in previous stud