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Two monoclonal antibodies (mAb) CB268 and CII-C1 to type 11 collagen (CII) react with precisely the same conformational epitope constituted by the residues ARGLT on the three chains of the CII triple helix. The antibodies share structural similarity, with most differences in the complementarity determining region 3 of the heavy chain (HCDR3). The fine reactivity of these mAbs was investigated by s

Vanadate and peroxovanadate (pV), potent inhibitors of tyrosine phosphatases, mimic several of the metabolic actions of insulin. Here we compare the mechanisms for the anti-lipolytic action of insulin, vanadate and pV in rat adipocytes. Vanadate (5 mM) and pV (0.01 mM) inhibited lipolysis induced by 0.01-1 microM isoprenaline, vanadate being more and pV less efficient than insulin (1 nM). A loss o

alpha 1-Antitrypsin (AAT), a major endogenous inhibitor of serine proteases, plays an important role in minimizing proteolytic injury to host tissue at sites of infection and inflammation. There is now increasing evidence that AAT undergoes post-translational modifications to yield by-products With novel biological activity. One Such molecule, the C-terminal fragment of AAT, corresponding to resid

Popular Abstract in Swedish Bröstcancer är den vanligaste maligna sjukdomen hos kvinnor i världen. Årligen insjuknar mer än 7300 kvinnor i Sverige, vilket innebär nästan 30 % av all kvinnlig cancer. Mer än 80 000 kvinnor i Sverige är friska efter att ha blivit behandlade för bröstcancer, och cirka 3000 kvinnor lever med återfall av sjukdomen. Prognosen har förbättrats de senaste decennierna, delviRadiotherapy (RT) after mastectomy has been part of the treatment for early breast cancer since the 1940s. The treatment reduces the risk of loco-regional recurrences, but implies risk of late side effects that can begin decades after RT. Aims of the thesis were: - To analyse the long term effects of postmastectomy radiotherapy and systemic treatment for 1100 stage II breast cancer patients in t

The indoor climate in buildings has changed in the last decade due to more efficient climatic systems, floor heating systems and larger open floor areas with more natural light. All this has induced increasing ranges of relative humidity between different seasons. Also with decreasing relative humidity (in the winter 30-50% RH, in the summer 70-90% RH), floor-heating systems increase the temper

Popular Abstract in Swedish Bakgrund En till fem procent av de patienter som genomgår öppen hjärtkirurgi får en efterföljande infektion i operationssåret och bröstbenet. Infektionen kan leda till allvarliga komplikationer såsom blodförgiftning, organsvikt och i värsta fall till att patienten avlider. De traditionella metoder som används för att behandla den här typen av infektion har visat sig haNegative pressure wound therapy (NPWT) has been widely adopted for the treatment of deep sternal wound infections due to its excellent clinical outcome. However, the mechanisms of action and effects on hemodynamics have not been thoroughly elucidated. The aims of the work presented in this thesis were, therefore, to examine the effects of NPWT on sternotomy wounds and on the intrathoracic organs.

We assessed age-related differences in adults in familiarization to treadmill walking within virtual environments (VE), and examined whether treadmill walking after familiarization resembles overground walking. Seventeen younger and 17 older adults walked at preferred speed on an overground walkway and afterwards walked at the same speed for 20 min on a treadmill coupled to a VE. A motion capture

OBJECTIVES: To investigate the distribution of the transient receptor potential (TRP) A1 ion channel in the rat urinary bladder, and to study the effects of hydrogen sulfide (H(2)S) and known TRPA1 activators on micturition in conscious rats and on heterologously expressed ion channels. METHODS: The expression of TRPA1 in urinary bladder was studied with fluorescence immunohistochemistry and real-

Background: Irritable bowel syndrome (IBS) and gastrointestinal (GI) dysmotility disorders have a similar clinical picture, although dysmotility disorders require the attention of a specialist. Patients with primary Sjogren's syndrome (pSS) have also been described to suffer from IBS-like symptoms. No objective marker is available to distinguish between the patients. A visual analogue scale has be

Popular Abstract in Swedish Den här avhandlingen handlar om ett protein, en receptor, kallad GPR30. GPR30 tillhör proteinfamiljen G protein-kopplade receptorer vilka är nödvändiga för att celler ska kunna kommunicera med varandra och med sin omgivning. Förenklat sätt kan de ses sitta på ytan av en cell där de väntar på att någonting ska binda till dem, vilket aktiverar receptorerna och får dem attThe large protein family called G Protein-coupled receptors (GPCRs) has co-evolved with life throughout evolution; from single cell organisms all the way to complex beings such as us humans. The fact that GPCRs are involved in essentially every physiological event, and that ~50% of drugs on the current market are either directly or indirectly targeted towards the function of GPCRs, we can be certa

Oxidative stress has been implicated as an important factor in the progression of many diseases. An imbalance in the redox-system may cause oxidation of cells and molecules, which might subsequently lead to tissue damage. The research presented in this thesis has focused on elucidating a possible involvement of the human protein alpha-1-microglobulin in the defence against pathological oxidation.

The potential of Fe-V-oxide catalysts for use in methanol oxidation is explored. Our results show that although FeVO4 is active and selective for formaldehyde formation, it is not completely stable towards volatilization under reaction conditions. Attempts to stabilize Fe-V-oxide were made using titania, alumina and silica supports. However, we observe that although some stabilization is achieved

There is a need to develop the enzymatic hydrolysis of cellulose for production of ethanol from biomass. In recent years the inhibitory effects of lignin in lignocellulosic substrates has been the focus of several studies. This points to the importance of understanding the interactions between cellulose degrading enzymes and lignin. Surface active substances have been shown to adsorb to lignin sur

In the search for a triggering mechanism for the Paleocene-Eocene (P-E) boundary event, 55 Ma, centimeter-resolution chemical (e.g., Ir, Os, Pt) and isotope (e.g., (OS)-O-187/Os-188, He-3/He-4, Sr-87/Sr-86) records across this boundary have been established for six uplifted marine sections in Egypt, Spain and Denmark. The sections studied represent some of the stratigraphically most complete recor

Human endogenous retroviruses (HERVs) and other long terminal repeat (LTR)–containing elements comprise a significant portion (8%) of the human genome and are likely vestiges of retroviral infections during primate evolution. Many of the HERVs present in human DNA have retained functional promoter, enhancer, and polyadenylation signals, and these regulatory sequences have the potential to modify t

A low-diversity halysitid-heliolitid autobiostrome from the Late Wenlock Halla Formation at Blåhäll 1, Gotland, indicates a brief period of particular environmental conditions. The reef is 0.3–0.5 m thick, internally without bedding structures, and had a sheet-like distribution on a low-relief soft-bottom, in marginal platform, graptolitiferous strata (height-width ratio is 1:900 in outcrop). The

The surfactant tetra(ethylene oxide) n-dodecyl amide (TEDAd) was compared with the poly(ethylene oxide) n-alkyl ethers C12E5, C10E5, and C12E4. The adsorption to hydrophobic liquid-vapor and solid-liquid interfaces, as well as the wetting behavior on a hydrophobic surface, was investigated. The amide group is more hydrophilic than the ethylene oxide group, raising the cloud point and critical mice

As the integrity of graft endothelium seems to be essential to successful long-term patency in coronary operations, its preservation demands the utmost care. The aim of the present study was to investigate the effects of currently used solutions on endothelium-dependent relaxation after short-term storage of vessels at room temperature or at 4 degrees C. The infrarenal rat aorta was selected for s

A large proportion of cells that proliferate in the adult dentate gyrus under normal conditions or in response to brain insults exhibit only short-term survival. Here, we sought to determine which cell death pathways are involved in the degeneration of newly formed neurons in the rat dentate gyrus following 2 h of electrically induced status epilepticus. We investigated the role of three families