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CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a

Perceptions of eHealth and digitalization among professional anaesthesia personnel : A Swedish national study

BACKGROUND: The objective of this study was to evaluate anaesthesia care professionals' perceptions and attitudes regarding the implementation and advancement of digital solutions in perioperative care.METHODS: Anaesthesia personnel working in public Swedish institutions where anaesthesia is administered were invited to respond to an online survey regarding their attitudes towards digitalization i

Service Life Assessment of Harbor Structures - Case studies of chloride ingress into concrete and sheet piling corrosion rates

Popular Abstract in English The two most used building materials in harbor structures are undoubtedly steel and concrete. These two materials are often combined in the structures of wharfs and quays where most steel sheet pile walls have cap beams of reinforced concrete. The degradation processes of these structures must be taken into account both when designing new structures and when inspecting The two most used building materials in harbor structures are undoubtedly steel and concrete. These two materials are often combined in the structures of wharfs and quays where the steel sheet pile walls often have cap beams of reinforced concrete. The degradation processes of these structures must be taken into account both when designing new structures and when inspecting existing harbor structu

CFD Modeling of Turbulent Flows in Industrial Applications with Emphasis on Premixed Combustion in Gas Turbines

Increasing restrictions on hazardous emissions combined with demands for good profitability from the operators in the energy market have contributed strongly to the development of innovations in which the emphasis is on low emissions and high efficiency. For gas turbine-based power plants, emission of NOx in particular is governed by stringent legislation. To meet these restrictions, most of the m

Natural course of knee osteoarthritis in middle-aged subjects with knee pain: 12-year follow-up using clinical and radiographic criteria

Objective: To explore the natural course of knee osteoarthritis (OA) in a middle-aged population with chronic knee pain. Methods: A population-based sample of 143 subjects (mean age 45 (range 35-54), 44% women) with knee pain (>3 months) at inclusion was studied. Weightbearing posteroanterior tibiofemoral (TF) radiographs were obtained at baseline and 12 years later, and classified according to Ke

Serum concentrations of human chorionic gonadotropin beta and its association with survival in patients with colorectal cancer

Increased serum concentrations of the beta subunit of human chorionic gonadotropin (hCG beta) are associated with adverse prognosis in several cancers. The aim of the present study was to analyse the association between serum hCG beta recurrence, and survival, in patients with colorectal cancer. The concentrations of hCG beta were determined in serum collected preoperatively from 324 patients with

Development of an Osmium Redox Polymer Mediated Bioanode and Examination of Its Performance in Gluconobacter oxydans Based Microbial Fuel Cell

Gluconobacter oxydans (G. oxydans) cells together with an osmium redox polymer (ORP) [Osmium (2,2'-bipyridine)2(poly-vinylimidazole)10Cl]Cl were combined with a glassy carbon paste electrode (GCPE) to form a bioanode for a microbial fuel cell (MFC) based on G. oxydans. Although there are G.oxydans/ ORP combined bioanode in the literature, as far as it is known, this system is the first one where G

Cardiovascular risk after hospitalisation for unexplained syncope and orthostatic hypotension

OBJECTIVE: To investigate the relationship of hospital admissions due to unexplained syncope and orthostatic hypotension (OH) with subsequent cardiovascular events and mortality.METHODS: We analysed a population-based prospective cohort of 30 528 middle-aged individuals (age 58±8 years; males, 40%). Adjusted Cox regression models were applied to assess the impact of unexplained syncope/OH hospital

Lika provocerande? - Provokationsbedömningen ur ett likabehandlingsperspektiv

Provokation som grund för strafflindring regleras i 29 kap. 3 § första stycket 1 BrB och då domstolen anser att någon objektivt sett har blivit utsatt för ett uppenbart kränkande beteende kan detta få verkan i förmildrande riktning i påföljdsbestämningen. Domstolar har ett relativt stort utrymme att fastställa vad som kan utgöra provokation på grund av att det finns få uttalanden i förarbeten och Provocation as a basis for mitigation of a sentence is regulated in Chapter 29. 3 § first paragraph 1 of the Penal Code and this can have effect in mitigating direction when the court considers that someone has been a victim of a clearly degrading behavior from an objective point of view. Courts have a relatively broad discretion to determine what may constitute provocation because there are only

Grundläggande rättssäkerhetskrav för dispute boards

Dispute boards är en form av privat tvistlösning som de senaste decennierna blivit populär både i Sverige och internationellt. Tanken med en dispute board är att låta en nämnd av privatdomare avgöra tvister på ett mindre formellt sätt än vad som är möjligt vid domstol eller skiljeförfarande och på så vis nå ett snabbt avgörande. Parter har större möjlighet att bestämma hur förfarandet ska gå till A dispute board is a type of private dispute resolution which in the last decades has achieved great popularity in Sweden as well as in an international context. The idea behind the dispute board is that a small board of private judges can solve disputes in a less formal manner than what is possible in litigation or arbitration. In this way a more rapid decision can be reached. Parties are in grea

Att vara ”varken-eller”

The purpose of this thesis is to study a group of sudanese men and women's view of so called "Swedishness" and if they perceive themselves as Swedish. I have examined how the informants identify themselves, and how they think others portray them. In general my informants show a clear view of ”Swedishness”. They see it as being impossible to identify as Swedish, due to the fact that t

Gemla Museum

This project is about creating a concept for a historical museum for the swedish furniture company Gemla AB. It will be a permanent exhibition located in connection to their factory. The reason Gemla wants to put up this museum is to give clients, students or the interested public a deeper understanding of the company and their history. My work will beto gather the parts in history that I think Ge

Bracing for the inevitable: An exploratory multi-case study of local climate change adaptation plans

Vulnerability to climate change and the capacity to adapt to it are the result of intersecting power structures, system dynamics, and social identity markers otherwise known as intersectionality; and thus, differ greatly between contexts. However, despite the stark contextual differences between small and metropolitan cities, and lower and higher income countries, the policy and study of climate c

Kan vi prata om det? Deliberativ demokrati, mångfald och politisk kamp

In recent years the liberal representative democracy has been subject of a substantial critique because of its inability to accommodate difference and create a genuinely democratic political process. One such critique comes from the theory of deliberative democracy. Supporters of deliberative democracy try to promote a model of democracy that take its vantage point in free and equal deliberation

Den rättvisa skatten - Ett etiskt argument för platt skatt

How should a fair income tax system be designed? Since Estonia became the first country to introduce a flat tax on incomes in 1994, this question has been in focus of public debate. The aim of is this thesis is to show that a flat income tax system, combining a proportional tax rate on all incomes with an allowance exempt from tax, is consistent with the demands of justice. In order to achieve thi

Vem vill inte vara solidarisk? Diskursiv förändring och betydelseförskjutning i EU:s energidebatt.

The aim of this master thesis is to examine the energy discourse of the EU. For this purpose history of concepts and discourse analysis is used. I show that the EU energy discourse is undergoing a change in the formulation of the Lisbon Treaty through the use of the concept of solidarity. This can be seen as the giving of a social dimension to the discourse and hence, a social dimension to the fut

Italiens förståelse för kvinnans arbetssituation, med orsak av de rådande stereotypa könsrollerna

The Italian society have severe problems with discrimination against women, and they are not showing any progress in the field. This text examines what understanding the Italian government shows for the deep rooted gender stereotypes and the impact those gender stereotypes has on womens working situation. To answer that I have studied their state report to the committee for Convention of the Elimi

En stundande skilsmässa?

In this essay I have examined the factors that may be underlying the fact that the co-operation between the Trade Union Confederation, LO, and the Social Democratic Workers Party, SAP, in Sweden is still strong. In Norway and Denmark this collaboration broke a few years ago and so I made an account of various factors that may have contributed to this break in Norway and Denmark, which I then compa

Integration of textile recycling with pulp mills - process design and optimization

Alltmedan produktionen av textiler kommit att öka under de senaste åren har dess miljöpåverkan blivit mer uppenbar. Eftersom dagens textilproduktion har negativa konsekvenser under produktion, användande, såväl som vid avfallshantering, så finns det ett behov av att hitta effektiva metoder för att ta tillvara på de resurser som investerats under produktionen. Idag förbränns eller deponeras textilaAs the production of textiles has increased over the years, the climate impact of the textile industry is becoming more evident. Textile production can have negative impacts on the environment, both during production, use and disposal, which calls for efficient recycling methods to recover some of the resources invested. However, textile waste is primarily incinerated or landfilled, which pose as