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Beta-galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis.

The enzyme beta-galactosidase was purified from a cold-adapted organism isolated from Antarctica. The organism was identified as a psychrotrophic Pseudoalteromonas sp. The enzyme was purified with high yields by a rapid purification scheme involving extraction in an aqueous two-phase system followed by hydrophobic interaction chromatography and ultrafiltration. The beta-galactosidase was optimally

Soil bacterial growth after a freezing/thawing event

Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9

TP53 mutations identify younger mantle cell lymphoma patients who do not benefit from intensive chemoimmunotherapy

Despite recent advances in lymphoma treatment, mantle cell lymphoma (MCL) remains incurable, and we are still unable to identify patients who will not benefit from the current standard of care. Here, we explore the prognostic value of recurrent genetic aberrations in diagnostic bone marrow (BM) specimens from 183 younger patients with MCL from the Nordic MCL2 and MCL3 trials, which represent curre

Modelling the external radiation exposure from the Chernobyl fallout using data from the Swedish municipality measurement system

In connection with the Chernobyl fallout and the subsequent deposition of radionuclides in Sweden, Swedish municipalities launched a measurement program to monitor the external radiation exposure. This program encompasses measurements of the ambient dose equivalent rate 1 m above ground at selected locations, and repeats those measurements at the same locations at 7-month intervals. Measurement da

Airspace dimension assessment with nanoparticles as a proposed biomarker for emphysema

Airspace dimension assessment with nanoparticles (AiDA) is a novel method to measure distal airspace radius non-invasively. In this study, AiDA radii were measured in 618 individuals from the population-based Swedish CArdiopulmonary BioImaging Study, SCAPIS. Subjects with emphysema detected by computed tomography were compared to non-emphysematous subjects. The 47 individuals with mainly mild-to-m

Biogeografisk uppföljning 2020 av dagfjärilar inom habitatdirektivet

Rapporten sammanfattar fjärilsinventeringar utförda 2020 inom Biogeografisk uppföljning som drivs av Naturvårdsverket. I fjällområdet kunde totalt 49 ytor inventeras. Sammanlagt noterades 151 högnordiska blåvingar på 22 inventerade ytor, 53 dvärgpärlemorfjärilar på 25 ytor och minst 1 fjällsilversmygare noterades på 3 inventerade ytor. Svartfläckig blåvinge observerades på 15 av 50 inventerade lok

Svensk Dagfjärilsövervakning, årsrapport för 2016

Detta är den sjunde årsrapporten från Svensk Dagfjärilsövervakning, ett nationellt miljöövervakningsprogram som koordineras av Lunds universitet på uppdrag av Naturvårdsverket sedan 2010. Svensk Dagfjärilsövervakning är ett samarbete mellan Sveriges Entomologiska Förening, Naturvårdsverket, Lunds universitet, Sveriges lantbruksuniversitet och Länsstyrelserna. Verksamheten möjliggörs av frivilliga

Islet adaptation in GIP receptor knockout mice

Glucose-dependent insulinotropic polypeptide (GIP) receptor knockout (KO) mice are tools for studying GIP physiology. Previous results have demonstrated that these mice have impaired insulin response to oral glucose. In this study, we examined the insulin response to intravenous glucose by measuring glucose, insulin and C-peptide after intravenous glucose (0.35 g/kg) in 5-h fasted female GIP recep

Predicted Heat Strain (PHS) model and the sweat loss in an extremely hot climate

Introduction: The aim was to study if the evaporative water loss can be predicted enough accurately for hydration recommendations by ISO 7933 – Predicted Heat Strain (PHS) model during a student laboratory exercise in an extremely hot environment.Method: Twelve young healthy students (8 males and 4 females), unacclimatized to heat, were exposed in a climatic chamber at 50˚C, 30% relative humidity

Biogeografisk uppföljning 2022 av dagfjärilar inom habitatdirektivet

Rapporten sammanfattar fjärilsinventeringar utförda 2022 inom Biogeografisk uppföljning som drivs av Naturvårdsverket. I fjällområdet kunde totalt 35 ytor inventeras. Sammanlagt noterades 38 högnordiska blåvingar på 12 av 16 inventerade ytor, 34 dvärgpärlemorfjärilar på 14 av 17 ytor och 3 fjällsilversmygare noterades på 2 av 3 inventerade ytor. Svartfläckig blåvinge observerades på 23 av 56 inven

Validation and correlation of high-sensitive troponin I and troponin T in the emergency department

Background: Troponin elevation is frequently observed in various scenarios in the Emergency Department (ED), yet there is a paucity of studies investigating simultaneously measured high-sensitivity cardiac troponin T (hs-cTnT) and troponin I (hs-cTnI) within a diverse cohort in a clinical setting. Methods: All patients who underwent troponin testing at a single center were eligible for this study.

Earthworm abundance increases aggregate stability : A field study in a Mediterranean agroforestry system

Soil aggregate stability has been asserted as an indicator of soil quality. Earthworms are essential for the stability of soil aggregates due to their activities, including burrowing and organic matter decomposition. In this study, we focused on how differences in plant litter quality influences earthworm abundance and composition and, subsequently, macroaggregate stability in an agroforestry ecos

Major immediate insertion-related complications after central venous catheterisation and associations with mortality, length of hospital stay, and costs : A prospective observational study

BACKGROUND: It is well-known that infectious complications after central venous catheterisation are associated with increased mortality, length of hospital stay and costs. However, there are limited data regarding such associations for immediate insertion-related complications. Therefore, the aim of this study was to investigate whether major immediate insertion-related complications are associate

Circulating lung-cancer-related non-coding RNAs are associated with occupational exposure to hexavalent chromium - A cross-sectional study within the SafeChrom project

BACKGROUND: Hexavalent chromium (Cr(Ⅵ)) is classified as a group 1 human carcinogen and increases the risk of lung cancer. Non-coding RNAs (ncRNAs) have key regulatory roles in lung cancer, but less is known about their relation to Cr(Ⅵ) exposure.OBJECTIVES: We aimed to 1) measure the expression of lung cancer-related circulating ncRNAs in exposed workers and controls; 2) assess associations betwe

Implementing Acute Stroke Services in Sub-Saharan Africa : Steps, Progress, and Perspectives from the Tanzania Stroke Project

Abstract – Introduction: Stroke is a leading cause of morbidity and mortality globally, with Africa bearing a disproportionately high burden of poor outcomes. In sub-Saharan Africa, acute stroke care remains inconsistent, with organized stroke units being either absent or rarely available, contributing to the high stroke mortality rates in the region. To address this issue, the Tanzania Stroke Pro

Association of glycemic control and chronic kidney disease with hospitalization in type 2 diabetes in a cross-sectional study in Region Halland

Aims: To examine the separate associations of glycemic control and chronic kidney disease with hospitalization days over one year in patients with type 2 diabetes (T2D). Materials and methods: A cross-sectional study using retrospective data on T2D patients in Region Halland, Sweden, during 2020. Data from the Region Halland database included hospitalizations, emergency visits, primary care encoun

Struggling to return to everyday life—The experiences of quality of life 1 year after delirium in the intensive care unit

Background: Many critically ill patients report a change in their health-related quality of life after intensive care unit (ICU) discharge. Patients who experience delirium during their ICU stay are perceived as a fragile group of ICU survivors, and the ‘quality of life’ phenomenon needs to be studied among these patients. Aim: To explore everyday life experiences of critically ill patients with d

Why tackling deforestation is so important for slowing climate change

Humanity injects an almost incomprehensible 42 billion tonnes of carbon dioxide (CO₂) into the atmosphere every year. The majority of this comes from burning fossil fuels, but a substantial portion, about 16%, arises from how we use the land. Most of these land-use emissions are caused by deforestation, particularly in the tropics.In order to slow climate change, the global community needs to redu

Low incidence of pelvic sepsis following Hartmann’s procedure for rectal cancer : a retrospective multicentre study

Background: Results of previous studies regarding pelvic sepsis after Hartmann’s procedure (HP) for rectal cancer have been inconsistent and few studies report the risk factors. This study aimed to investigate the incidence of pelvic sepsis after HP, identify risk factors and describe when as well as how pelvic sepsis was diagnosed and treated. Methods: Data were collected from the Swedish Colorec

Variation in Patient-Reported Outcomes in Young and Old Patients Up to 4 to 6 Years After Arthroscopic Partial Meniscectomy

Objective: To assess the variation in changes in patient-reported outcomes 4 to 6 years after arthroscopic partial meniscectomy (APM).Design:Prospective cohort study.Setting:Orthopedic departments at public hospitals.Patients:Patients (n = 447) from the Knee Arthroscopy Cohort Southern Denmark undergoing APM.Interventions:All patients underwent APM.Main Outcome Measures:Change in KOOS4scores from