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Site-specific cancer deaths in cancer of unknown primary diagnosed with lymph node metastasis may reveal hidden primaries.

Site use by dark-bellied brent geese Branta bernicla bernicla on the Russian tundra as recorded by satellite telemetry: implications for East Atlantic Flyway conservation

Waterbird phenology and opportunistic acceptance of a low quality staging site at Lake Baikal, eastern Siberia

Structure and dynamics of the active site of hen egg-white lysozyme from atomic resolution neutron crystallography

Hen egg-white lysozyme (HEWL) is a widely used model protein in crystallographic studies and its enzymatic mechanism has been extensively investigated for decades. Despite this, the interaction between the reaction intermediate and the catalytic Asp52, as well as the orientation of Asn44 and Asn46 side chains, remain ambiguous. Here, we report the crystal structures of perdeuterated HEWL and D2O b

The Cu_B site in particulate methane monooxygenase may be used to produce hydrogen peroxide

The spatial consistency and repeatability of migratory flight routes and stationary sites of individual European nightjars based on multiannual GPS tracks

Structural and biochemical insight into the mechanism of dual CpG site binding and methylation by the DNMT3A DNA methyltransferase

Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation

ATP phosphoribosyltransferase catalyses the first step of histidine biosynthesis and is controlled via a complex allosteric mechanism where the regulatory protein HisZ enhances catalysis by the catalytic protein HisGS while mediating allosteric inhibition by histidine. Activation by HisZ was proposed to position HisGS Arg56 to stabilise departure of the pyrophosphate leaving group. Here we report

The evolution of multiple active site configurations in a designed enzyme

Developments in computational chemistry, bioinformatics, and laboratory evolution have facilitated the de novo design and catalytic optimization of enzymes. Besides creating useful catalysts, the generation and iterative improvement of designed enzymes can provide valuable insight into the interplay between the many phenomena that have been suggested to contribute to catalysis. In this work, we fo

De novo active sites for resurrected Precambrian enzymes

Active Site Hydrophobicity and the Convergent Evolution of Paraoxonase Activity in Structurally Divergent Enzymes : The Case of Serum Paraoxonase 1

Computational Study of the pK_a Values of Potential Catalytic Residues in the Active Site of Monoamine Oxidase B

Monoamine oxidase (MAO), which exists in two isozymic forms, MAO A and MAO B, is an important flavoenzyme responsible for the metabolism of amine neurotransmitters such as dopamine, serotonin, and norepinephrine. Despite extensive research effort, neither the catalytic nor the inhibition mechanisms of MAO have been completely understood. There has also been dispute with regard to the protonation s

Completion of registration of risk factor variables during telephone vs on-site follow-up after myocardial infarction : a nationwide observational study in 101 199 patients from contemporary clinical practice in Sweden

Buxus sempervirens and Laurus nobilis leaves from an archaeological site at 7300 cal BP (La Draga, Spain) provide a better understanding of past vegetation and human activities

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

Site-dependent nuclear dynamics in core-excited butadiene

Prey capture by the non-native carnivorous pitcher plant Sarracenia purpurea across sites in Britain and Ireland

The carnivorous pitcher plant Sarracenia purpurea is native to North America, but has been introduced into Europe, where it is now widespread. Understanding of how this species functions in its non-native range is limited. We measured pitcher morphology and prey capture by S. purpurea in its non-native range in Britain and Ireland. Pitchers were removed from different plants at each of six bogs co

Site-specific N-glycan profiles of α₅β₁ integrin from rat liver

Making and Contesting Far Right Sites of Memory : A Case Study on Romania

The protein S-binding site localized to the central core of C4b-binding protein