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Immune response in deep cervical lymph nodes and spleen in the mouse after antigen deposition in different intracerebral sites

Brain interstitial and cerebrospinal fluid drainage into the lymphatics was studied by injections of 5 microliters of packed sheep red blood cells (SRBC) injected into the caudate nucleus, the occipital lobe, and the lateral ventricle of the brain in mice. The number of plaque-forming cells (PFC) was determined in the deep cervical lymph nodes, the axillary lymph nodes, and the spleen, and the num

Naphthyl Thio- and Carba-xylopyranosides for Exploration of the Active Site of β-1,4-Galactosyltransferase 7 (β4GalT7)

Xyloside analogues with substitution of the endocyclic oxygen atom by sulfur or carbon were investigated as substrates for β-1,4-galactosyltransferase 7 (β4GalT7), a key enzyme in the biosynthesis of glycosaminoglycan chains. The analogues with an endocyclic sulfur atom proved to be excellent substrates for β4GalT7, and were galactosylated approximately fifteen times more efficiently than the corr

Large differences between carbon and nutrient loss rates along the land to ocean aquatic continuum—implications for energy : nutrient ratios at downstream sites

The balance between the availability of energy and nutrients is decisive for the growth and survival of organisms. Here, we evaluated how energy, in this study expressed as total carbon (TC), is lost along the land to ocean aquatic continuum (LOAC) in relation to nutrients, i.e., total phosphorus (TP), total nitrogen (TN), total iron (TFe), and dissolved silica (DSi). For the evaluation, we used d

Urban seismic site investigations for a new metro in central Copenhagen: Near surface imaging using reflection, refraction and VSP methods.

Planning and execution of large infrastructure projects benefit from detailed mapping of the subsurface through geophysical methods. However applying geophysical methods in an urban environment requires careful preparation to ensure success in areas where source of noise and present infrastructure may compromise the results. We present and discuss the results of a seismic investigation conducted i

Life Cycle Assessment of an Office Building Based on Site-Specific Data

Life cycle assessment (LCA) is an established method to assess the various environmental impacts associated with all the stages of a building. The goal of this project was to calculate the environmental releases for a whole office building and investigate the contribution in terms of environmental impact for different parts of the building, as well as the impact from different stages of the life cLife cycle assessment (LCA) is an established method to assess the various environmental impacts associated with all the stages of a building. The goal of this project was to calculate the environmental releases for a whole office building and investigate the contribution in terms of environmental impact for different parts of the building, as well as the impact from different stages of the life c

From lab-scale to on-site pilot trials for the recovery of hemicellulose by ultrafiltration: Experimental and theoretical evaluations

Spent sulfite liquor (SSL) is a byproduct of the sulfite pulping process of wood. SSL usually contains monosugars and lignosulfonates, which are fermented to produce ethanol and dried to generate lignosulfonate salts. However, the SSL that was used in this work was derived from the first step of a 2-step sulfite pulping process of softwood under mild pulping conditions in the first stage of cookin

Protein Folding Studied by Site Directed Mutagenesis

The N-terminal RNA binding domain of U1A has been shown to fold in a two-state process without accumulating any intermediates. U1A has a symmetrically curved chevron plot which seems to result mainly from changes of the transition state. At low concentrations of denaturant the transition state occurs early in the folding reaction, whereas at high denaturant concentration it moves close to the nati