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Bacteria and humans have co-evolved since their common existence. They have thereby developed very specific defence and target mechanisms against one another. Understanding the complex biomolecular processes behind these interactions can be of great use for developing treatments against bacterial diseases. The aim of this thesis project was to verify, and introduce a model for understanding, the i
Monoamine oxidase (MAO), which exists in two isozymic forms, MAO A and MAO B, is an important flavoenzyme responsible for the metabolism of amine neurotransmitters such as dopamine, serotonin, and norepinephrine. Despite extensive research effort, neither the catalytic nor the inhibition mechanisms of MAO have been completely understood. There has also been dispute with regard to the protonation s
Lund University conducts active fundraising to increase philanthropic funding for the university's activities. Target groups are private foundations, companies, organizations and individuals. The Development Office is the central hub for generating these types of donations and gifts. Our overall goal with the operation is that Lund University and our donors, in their own unique way, should be able
Pentaklorfenol (PCP) är en klorerad aromatisk förening som användes flitigt för att behandla trä mot mikrobiell nedbrytning innan den blev förbjuden på 70-talet. På grund av dess höga löslighet förekommer PCP som en grundvattenförorening kring äldre sågverk. Hjortsberga i södra Sverige är ett exempel på denna typ av förorening och är det område som undersöks i arbetet. Förorenat grundvatten spridsPentachlorophenol (PCP) is a chlorinated aromatic compound that was commonly used for treatment of wood against microbial degradation before it got banned in the 1970s. Due to its solubility in water, PCP is a common groundwater contaminant around old sawmills, for example near Hjortsberga in southern Sweden where this study was performed.. The contaminated groundwater is spreading and leaking int
Extensive research is being conducted on magnesium (Mg) alloys for bone implant manufacturing, due to their biocompatibility, biodegradability and mechanical properties. Gadolinium (Gd) is among the most promising alloying elements for property control in Mg alloy implants; however, its toxicity is controversial. Investigating Gd behavior during implant corrosion is thus of utmost importance. In t
The World Meteorological Organization (WMO) Global Atmosphere Watch (GAW) coordinates high-quality atmospheric greenhouse gas observations globally and provides these observations through the WMO World Data Centre for Greenhouse Gases (WDCGG) supported by Japan Meteorological Agency. The WDCGG and the National Oceanic and Atmospheric Administration (NOAA) analyse these measurements using different
BdbD is a thiol: disulfide oxidoreductase (TDOR) from Bacillus subtilis that functions to introduce disulfide bonds in substrate proteins/peptides on the outside of the cytoplasmic membrane and, as such, plays a key role in disulfide bond management. Here we demonstrate that the protein is membrane-associated in B. subtilis and present the crystal structure of the soluble part of the protein lacki
The X-ray structures of dUTPase from equine infectious anaemia virus (EIAV) in unliganded and complexed forms have been determined to 1.9 and 2.0 A resolution, respectively. The structures were solved by molecular replacement using Escherichia coli dUTPase as search model. The exploitation of a relatively novel refinement approach for the initial model, combining maximum likelihood refinement with
Leukotriene ( LT) A(4) hydrolase is a bifunctional zinc metalloenzyme, which converts LTA(4) into the neutrophil chemoattractant LTB4 and also exhibits an anion-dependent aminopeptidase activity. In the x-ray crystal structure of LTA(4) hydrolase, Arg(563) and Lys(565) are found at the entrance of the active center. Here we report that replacement of Arg(563), but not Lys(565), leads to complete a
