We investigated the effects of UDCA on sphingomyelinase (SMase) in Caco 2 cells cultured in monolayer and polarized conditions. Alkaline SMase activity was high in polarized cells whereas. acid and neutral SMase activities were high in monolayer cells. In polarized cells, UDCA increased alkaline SMase expression and caspase 3 activity but had no effect on acid and neutral SMases. In monolayer cell

This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcbeta1-3GalNAc). Among the terminal saccharide determinants Sd(a)/Cad-antigen-like structures were prominent, and Lewis x, sialyl Lewis x and sulphated Lewis x were found as minor compo

We present experiments on field-free molecular alignment of N-2 and CO2 probed with short XUV pulses that are obtained via high-harmonic generation. The XUV pulses induce a dissociative ionization or a Coulomb explosion of the molecule, where the fragment ion recoil (measured using the velocity map imaging technique) provides information on the alignment of the parent molecule at the time of ioniz

The conditional subjunction "äss" 'if' is used in many Swedish dialects. The article discusses its etymology and the connection between various phonetic variant as well as the process of its grammaticalization.

Latin poetry in the service of political propaganda. The poems commissioned by princes from hired writers of Latin verse had the same function as propagandist media and planted information has today. A survey from the Augustean times up till the 19th century.

Popular Abstract in Swedish Avloppsvatten renas från fosfor för att minimera utsläppet till sjöar och hav. Näringsämnena fosfor och kväve bidrar till övergödningen i våra vatten och kan reduceras kraftigt på avloppsreningsverken. Fosforavskiljning går huvudsakligen ut på att få löst fosfat att hamna i en fast fas ? slammet, för att sedan kunna avskilja det från det renade vattnet. Bakterier som vThe idea of phosphorus removal at wastewater treatment plants is to transfer dissolved phosphorus into a solid phase. Together with the rest of the produced sludge it is then removed from the water, resulting in a decreased concentration of phosphorus in the effluent and the prevention of the eutrophication in the recipient. Phosphorus can either be stored biologically, with enhanced biological p

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Normal ageing in men is associated with a physiological decline in testosterone levels, but adverse environmental or psychosocial factors may also impair testosterone secretion and cause secondary biological changes. In a number of observational or experimental studies in both animals and man, it has been possible to study the influences on gonadal function of stressful life situations. To underst

Accurate and precise determinations of thermodynamic parameters of binding are important steps toward understanding many biological mechanisms. Here, a multi-method approach to binding analysis is applied and a detailed error analysis is introduced. Using this approach, the binding thermodynamics and kinetics of the reconstitution of the protein monellin have been quantitatively determined in deta

Local mode spectroscopy and ab initio modeling are used to investigate two trigonal defects found in carbon rich Si into which H had been in-diffused. Isotopic shifts with D and C-13 are reported along with the effect of uniaxial stress. Ab-initio modeling studies suggest that the two defects are two forms of the CH2* complex where one of the two hydrogen atoms lies at an anti-bonding site attache

We present a new probability density function (PDF)-based computational model to simulate a homogeneous charge compression ignition (HCCI) engine with direct injection (DI) during gas exchange. This stochastic reactor model (SRM) accounts for the engine breathing process in addition to the closed-volume HCCI engine operation. A weighted-particle Monte Carlo method is used to solve the resulting PD

Popular Abstract in Swedish Avhandlingen beskriver hur man med elektromagnetisk reflekterar strålning, registrerad från satelliter vilka cirkulerar runt jorden kan lokalisera och kvantifiera skador på barrskog i Tjeckien. Signifikanta samband mellan reflektans och barrförluster gör det mögligt att utföra översiktliga uppskattningar av dessa varrförluster med satellitdata. Genom att jämföra skogsThis thesis describes the localisation and quantification of deforestation and forest damage in Norway spruce forests in northern Czech Republic using Landsat data. Severe defoliation increases the spectral reflectance in all wavelength bands, especially in the mid infrared region. These spectral differences allow the separation of three damage categories with an accuracy of 75% using TM data and

A case study is presented in which two organisations have participated in a benchmarking initiative to discover improvement suggestions for their processes for managing product platforms. The initiative, based on an instrument which consists of a list of questions, has been developed as part of this study and contains eight major categories of questions that guide the participating organisations t

BACKGROUND: Dietary factors are thought to have an important role in gastric and esophageal carcinogenesis, but evidence from cohort studies for such a role is lacking. We examined the risks of gastric cancer and esophageal adenocarcinoma associated with meat consumption within the European Prospective Investigation Into Cancer and Nutrition (EPIC) cohort. METHODS: A total of 521,457 men and women

An impulse-oscillation model (IOM) was used for a time description of catalytic acts in the reactions of catalytic decomposition of formic acid on oxide catalysts. The results of computation using the model show that for the dehydrogenation reaction the most important modes are the OCO bending in bidentate adsorbed formate ion and OH stretching on the catalyst surface while for the dehydration the

Theoretical and experimental evidence is presented which leads to a rejection of the proposal that the transient-state kinetics of the coupled two-enzyme reaction involving aldolase and glyceraldehyde-3-phosphate dehydrogenase are indicative of a channelled transfer of glyceraldehyde 3-phosphate from the producing enzyme to the consuming one.