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A new type of support for chromatographic use has been developed. Non-porous quartz fibre, with a mean diameter of 0.5 μm, was silylated with mercaptopropyltrimethoxysilane. Tresyl chloride-activated dextran was covalently coupled to the SH groups on the fibre. Remaining active tresyl groups on the dextran were then coupled with an NAD derivative.The affinity fibre contained 0.3 μmol NAD derivativ

A new technique to speed up the phase separation of aqueous two-phase systems is described. The technique is based on the addition of magnetically susceptible additives (ferrofluids or iron oxide particles). In a magnetic field such additives will induce a faster phase separation. In one approach, dextran-stabilized ferrofluid was added to an aqueous two-phase system containing polyethylene glycol

BACKGROUND: Anemia has been suggested to be related with schooling outcomes in India. Less is known, however, about whether the observed relationship persists after accounting for all household-level factors which may confound the association between anemia and schooling.METHODS: Nationally representative data on adolescents aged 15-18 years with data on measured hemoglobin level and school attend

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Three naturally occurring isomers of the disaccharideO-α-D-mannosyl-D-mannoside were synthesized by reversing the hydrolytic activity of jack bean α-mannosidase at 75°C in a very high concentration of mannose. Higher oligosaccharides were also obtained at the later stages of the reaction. The maximum total yield of disaccharides was 37% (w/w) based on the total amount of saccharides.

Anmeldelse af Senseis Mappe af Hiromi Kawakami

BackgroundThere is a growing body of evidence indicating that second degree tears cause both short-, and long-term consequences. Very few preventative measures have been found to reduce the incidence of these tears.ObjectiveTo investigate if the use of a perineal protection device during vaginal birth reduces severe perineal tearing (≥grade 2 tear) in primiparous women compared to routine manual p

The disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose has been synthesized by transfer of the β-d-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-d-galactose utilizing the transferase activity of β-galactosidase from . To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%,

A crucial aspect of adding an economical and environmental dimension to the upgrading of bio-oils is to develop catalysts with enhanced and prolonged activity. In the present study, the effect of doping δ-alumina (Al2O3) with oxides of cerium (Ce) and lanthanum (La) before thermal treatment was investigated. The performance of such an Al2O3-supported nickel-molybdenum (Ni-Mo) catalyst was evaluate

Affinity precipitation, a novel technique closely related to immunoprecipitation and affinity chromatography, has been evaluated in systems comprised of dehydrogenases and a bifunctional NAD derivative, Bis-NAD. Lactate dehydrogenase and glutamate dehydrogenase were easily precipitated whereas yeast alcohol dehydrogenase required the presence of salt to enhance the affinity precipitation. Liver al

Aims/hypothesis: Regulatory factor X 6 (RFX6) is crucial for pancreatic endocrine development and differentiation. The RFX6 variant p.His293LeufsTer7 is significantly enriched in the Finnish population, with almost 1:250 individuals as a carrier. Importantly, the FinnGen study indicates a high predisposition for heterozygous carriers to develop type 2 and gestational diabetes. However, the precise

The complement system is a proteolytic cascade triggered by pathogen and danger-associated molecular patterns, with resultant outcomes of inflammation, cellular activation, and opsonization of material for removal by phagocytosis. While first discovered as an activity in serum, it is now recognized that complement components play important roles at local and individual cell-intrinsic levels. In pa