Continuous multicomponent quantification during supercritical fluid extraction applied to microalgae using in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection
A quantitative methodology based on in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection for supercritical fluid extraction is proposed. The method was applied to the extraction of carotenoids, chlorophyll A, ergosterol and total lipids from microalgae. One regression technique and two curve resolution techniques were applied on the absorption spectroscopy data