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Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat

Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in

Molecular genetic analysis of severe protein C deficiency

Severe protein C deficiency is a rare, early onset, venous thrombotic condition that is inherited as an autosomal recessive trait. The protein C (PROC) genes of nine unrelated individuals with severe protein C deficiency were sequenced yielding a total of 13 different lesions. Eight of these were novel, including a gross gene deletion, three missense mutations, two micro-deletions, a splicing muta

Homozygosity for the IgG2 subclass allotype G2M(n) protects against severe infection in hereditary C2 deficiency

Homozygous C2 deficiency (C2D) is the most common deficiency of the classical complement pathway in Western countries. It is mostly found in patients with autoimmune disease or susceptibility to bacterial infections and in healthy persons. We wished to assess to what extent other immunological factors might explain differences of susceptibility to infections in C2D. For this reason, 44 Swedish pat

Interpolation classes and matrix monotone functions

An interpolation function of order n is a positive function -/+ on (0, infinity) such that vertical bar vertical bar -/+ (A)(1/2) T -/+ (A)-(1/2) vertical bar vertical bar <= max(vertical bar vertical bar T vertical bar vertical bar, vertical bar A(1/2)TA(-1/2) vertical bar vertical bar) for all n x ii matrices T and A such that A is positive definite. By a theorem of Donoghue, the class C-n of in

Measuring the size of ulcers by planimetry: a useful method in the clinical setting

OBJECTIVE: The aim of this study was to compare four measurement methods. Digital planimetry was compared with mechanical planimetry, placing the film over graph paper and counting the squares (grid tracing), and multiplying the two maximal perpendicular diameters of the ulcer (diameter product). METHOD: Twenty patients with 50 chronic leg ulcers of various aetiology and sizes (20 ulcers were < or

No title

OBJECTIVE: To study the variation of HCV 5'-NCR and core gene in serial sera collected from patients with HCV infection. METHODS: Serial sera were collected from 4 plasma-donors with HCV infection. 5'-NCR and core gene were amplified, sequenced and analyzed using software. RESULTS: All the sequences obtained from the serial sera of patients with HCV infection were 1b and 2a subtypes. The variation

Striatal neuron differentiation from neurosphere-expanded progenitors depends on Gsh2 expression.

Neural stem and progenitor cells from the embryonic forebrain can be expanded under growth factor stimulation in vitro, either as free-floating aggregates called neurospheres or as attached monolayer cultures. We have previously shown that despite the maintenance of important regulatory genes such as Gsh2, in vitro expansion of cells from the lateral ganglion eminence (LGE) restricts their differe

Electrical and thermal characterization of a PV-CPC hybrids

Long term evaluation of an asymmetric CPC PV-thermal hybrid built for high latitudes, MaReCo (MaximumReflectorCollector), is performed in Lund, lat 55.7o, and this paper discusses output estimates and characteristics of the system. The output estimates are calculated using the MINSUN simulation program. To get the input for MINSUN, measurements were performed on two MaReCo prototypes. These measur

Intranät : En ny arena för kommunikation och lärande

Popular Abstract in Swedish I Sverige har intranät inom loppet av ett par år införts i drygt hälften av alla organisationer – privata och offentliga. Intranät har av många setts som ett verktyg för att effektivisera kommunikationen och lärandet. Behovet av effektiv kommunikation och lärande har accelererat under 1990-talet med en allt större konkurrens mellan organisationerna. En god tillgång tillDuring the 1990s, communication and learning attracted heightened attention in organizational contexts. Generally speaking, there is great faith in the significance of ICT for people’s learning. One form of technology which has had a great impact on Swedish organizations is the intranet. Within the course of a few years, intranets have been implemented in just over half of all Swedish organization

A Mesoeconomic Analysis of the Construction Sector

The fundamental question posed in this study is how to approach the construction sector in order to cover its wide scope of activities, its great variety of actors and the external influences on it from the market and institutional environment. Construction includes such diverse economic activities as new production and as repair/maintenance, itinerant production and stationary manufacture, the pr

Quantum behavior in nanoscale ballistic rectifiers and artificial materials

Low-temperature experiments are performed on nanoscale nonlinear devices (ballistic rectifiers) as well as nanostructured artificial materials, fabricated from an InP/InGaAs quantum well wafer. A dc output is generated between the lower and upper contacts of these devices, when an ac voltage is applied between the left and right contacts. As the temperature is lowered from room temperature, the dc

Effect of osmotis pretratment and pulsed electric field on the viscoelastic properties of potato tissue.

Compression stress relaxation of potato tissue exposed to pulsed electric field (PEF) treatment, with or without osmotic pretreatment, was measured and modelled with five parameter generalised Maxwell model. The changes in viscoelastic model coefficients were quantified as a response to applied field strength, pulse length, and pulse number and were correlated with conductivity changes. Using the