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Fused-silica capillaries chemically derivatized with silane/poly(vinylpyrrolidone) (PVP) or dynamically modified with plexiglas [poly(methyl methacrylate)] were prepared and evaluated with regard to column stability and separation performance for capillary isoelectric focusing of standard proteins. The PVP coating showed the better stability and was good for at least 100 runs while the plexiglas c
Drug-induced dyskinesias (DID) manifested as hyperkinetic and/or dystonic movements or postures are common problems in Parkinson's disease (PD). Novel therapeutic interventions may offer possibilities to counteract these common adverse effects of an otherwise necessary treatment. To be able to evaluate the effects of such interventions on DID, reliable and relevant clinical assessment tools are ne
Uptake of arsenic, molybdenum, uranium and vanadium by species of natural vegetation (Agrostis capillaris, Betula pendula, Calluna vulgaris, and Deschampsia flexuosa) on two contrasting, highly acid soils (pH of soil solution 4.2-4.3), differing in natural abundance of these elements, was compared. the soil developed from alum shale was rich in these elements, the soil from a gneiss moraine was po
Recent experiments uncovered a mutational pathway between two proteins, along which a single mutation causes a switch in fold. Searching for such paths between real proteins remains, despite this achievement, a true challenge. Here, we analyze fold switching in the minimalistic hydrophobic/polar model on a square lattice. For this analysis, we generate a comprehensive sequence-structure database f
The possible teratogenic effect of erythromycin therapy, noted previously, was studied. Women who had taken erythromycin or penicillin V in early pregnancy and their infants were studied, using the Swedish Medical Birth Register where information on drug use during pregnancy was recorded based on interviews in early pregnancy. The risk for any congenital malformation after erythromycin therapy (bu
Biosensors for measurement of glycerol in FIA were constructed using NAD(+)-dependent glycerol dehydrogenase (GlDH) either co-immobilized with phenazine methosulphate (PMS) or cross-linked to an Os-complex-modified poly(vinylimidazole) redox polymer (PVI(13)dmeOs) using poly(ethyleneglycole) diglycidilether (PEGDGE). The GlDH/PMS sensor was characterized by a linear range of 0.01-1mM glycerol, a s
Using a new channel shape equation for straight channels and a more versatile channel shape or local flow depth equation for natural streams a method is developed for prediction of the longitudinal dispersion coefficient in single-channel natural streams, including straight and meandering ones. The method involves derivation of a new triple integral expression for the longitudinal dispersion coeff
beta-Carbolines show structural resemblance to the neurotoxic N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and are metabolized to mitochondrial toxicants. Humans are continuously exposed to low levels of beta-carbolines through cooked food, coffee, alcoholic beverages and tobacco smoke. beta-Carbolines have previously been detected in higher levels in the pigmented substantia nigra than in the cor
Introduction Manual interpretation of immunohistochemistry (IHC) is a subjective, time-consuming and variable process, with an inherent intra-observer and inter-observer variability. Automated image analysis approaches offer the possibility of developing rapid, uniform indicators of IHC staining. In the present article we describe the development of a novel approach for automatically quantifying o
During drying, timber distortion is a major defect mainly due to shrinkage anisotropy, differences in longitudinal shrinkage, and spiral grain. The distortion can be reduced by external restraint and use of appropriate kiln schedules. The research presented here is part of a project on the improvement of shape stability of Norway spruce by high-temperature drying. The effects of drying temperature
A tissue proteomics process is presented where hepatocyte cell isolation in combination with two-dimensional (2-D) gel electrophoresis and mass spectrometric identification were used to annotate the liver proteome. Laser microdissection of 8 m liver tissue sections was performed and protein expression profiling was compared using a variety of quantities of input cells, and gel separation condition
The present study is one of a few that have used "sense of coherence" (SOC) as a dependent variable in an explanatory model. After studying three different samples - 680 students, 180 parents and 315 couples - we conclude that family relational and psychopathological variables contribute significantly to the explanation of SOC (explained variance between 10-27 and 26-50 per cent). In total, we obt
We have estimated the activation energy for hydrogen abstraction by compound I in cytochrome P450 for a diverse set of 24 small organic substrates using state-of-the-art density functional theory (B3LYP). We then show that these results can be reproduced by computationally less demanding methods, for example, by using small organic mimics of compound I with both B3LYP and the semiempirical AM1 met
We have previously raised two monoclonal antibodies (7B1, 14F11) recognizing the high-affinity leukotriene B4 receptor, BLT1. They were presently used to determine receptor surface expression in the hematopoietic system. In peripheral blood, BLT1 was primarily recognized in granulocytes, monocytes and, to a lower extent, in certain lymphocytes except the CD4 subpopulation. The expression pattern w
Abstract in UndeterminedDiabetic foot complications result in huge costs for both society and the individual patients. Few reports on the health-economic consequences of diabetic foot infections have been published. In studies considering a wide societal perspective, costs of antibiotics were relatively low, whereas total costs for topical treatment were high relative to the total costs of foot in
