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Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and
The effect of freestream turbulence on the flow past a circular cylinder (tube) was studied experimentally for Reynolds numbers between 2 x 104 and 6 x 104. Investigations were carried out for uniform (non-turbulent) flow and for turbulent flows having turbulence intensities between 1.3 and 4.1%. The variations in the pressure forces and shedding frequencies were found to be related to the variati
While sensory perception is currently receiving quite a lot of attention across disciplines, little empirical research has focused on how people describe auditory experiences. Research on the representation of sound in fiction narratives has shown that sounds are frequently conceptualized as events whose descriptions are instantiated in domains other than sound proper, as in strong men banging the
Cellular localization has received marked attention in academia and in industry, as indicated by a rapidly expanding volume of literature specific to vehicular environments. Impressive results have been shown for a number of problems as measured by metrics such as accuracy and latency. This article identifies five important requirements for cellular localization for safety-critical systems with a
Along with the ever-expanding interest in brands, the brand orientation concept has since its inception in the early 1990s steadily received more and more attention from scholars and practitioners alike. Brand orientation ultimately captures how organisations can develop sustainable competitive advantages in the form of brands. However, the conditions for brand management have dramatically changed
Sharing is considered as one of the key elements in circular economy, as well as away to deliver social impact. According to the New Bauhaus initiative by the EuropeanCommission, new neighbourhoods should be environmentally sustainable, provide anaesthetic experience, and deliver social inclusion. This study explores the emergence of sharing business models in the real estate industry. The aim is
Kinesthetic teaching allows human operators to reprogram part of a robot’s trajectory by manually guiding the robot. To allow kinesthetic teaching, and also to avoid any harm to both the robot and its environment, Cartesian impedance control is here used for trajectory following. In this paper, we present an online method to modify the compliant behavior of a robot toward its environment, so that
Introduction. In policies and research, public libraries are often put forth as public spheres promoting inclusion and shared values. This article investigates possible implications of replacing the idea of the library as public sphere with a plurality of public spheres, thereby acknowledging existing inequalities and conflicts between adversaries.Method. The paper is conceptual, focusing on deepe
The past two decades have seen unprecedented student demand for global health education through experiential and engaged learning within institutions of higher education in the United States. This demand created a need for innovative institutional partnerships. Since 2007, faculty from Cornell University’s Global Health Program and Kilimanjaro Christian Medical University College (KCMUCo) have wor
Purpose or ObjectiveTo evaluate two commercial, CE labeled deep learning-based models for automatic organs at risk segmentation on planning CT images for prostate cancer radiotherapy. Model evaluation was focused on assessing both geometrical metrics and evaluating a potential time saving.Material and MethodsThe evaluated models consisted of RayStation 10B Deep Learning Segmentation (RaySearch Lab
Universal Design for Learning (UDL) is considered an approach to include all students in the university. UDL is based on three main principles providing multiple means of Engagement, multiple means of representation, and multiple means of action and expression. It is relevant for teachers and departments to focus on more flexibility for students to get the required information, expressing their th
Sedimentary molybdenum (Mo) and uranium (U) enrichments are widely used to reconstruct changes in bottom water oxygen conditions in aquatic environments. Until now, most studies using Mo and U have focused on restricted suboxic-euxinic basins and continental margin oxygen minimum zones (OMZs), leaving mildly reducing and oxic (but eutrophic) coastal depositional environments vastly understudied. C
Recent findings support the hypothesis that inhibition of SMYD3 methyltransferase may be a therapeutic avenue for some of the deadliest cancer types. Herein, active site-selective covalent SMYD3 inhibitors were designed by introducing an appropriate reactive cysteine trap into reversible first-generation SMYD3 inhibitors. The 4-aminopiperidine derivative EM127 (11C) bearing a 2-chloroethanoyl grou
