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Miniaturized, Membrane-Based and Environmentally Green Sample Preparation Systems: Applications for Biological and Environmental Aqueous Samples

Contemporary advancements in miniaturization of analytical systems have advantageously fostered development, automation, and hyphenation of a variety of sample preparation techniques. Among several downscaled extraction designs, porous synthetic polymeric membranes (such as flat sheet (FS) or hollow fiber (HF)) have been utilized for membrane-based extraction, separating two aqueous phases (as in

Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. Among the mechanisms by which these inhibitors may act are through interfering wi

An ex Vivo Model for Evaluating Blood-Brain Barrier Permeability, Efflux, and Drug Metabolism.

The metabolism of drugs in the brain is difficult to study in most species because of enzymatic instability in vitro and interference from peripheral metabolism in vivo. A locust ex vivo model that combines brain barrier penetration, efflux, metabolism, and analysis of the unbound fraction in intact brains was evaluated using known drugs. Clozapine was analyzed, and its major metabolites, clozapin

Geographic variations in possible risk factors for severe cardiac malformations

The aim of this study was to investigate various putative risk factors in a county in Sweden, described as having a 28% increased prevalence of cardiovascular malformations, and to compare them with the risk factors in two reference counties. Women giving birth in the studied counties differed in age and parity distribution, smoking, and educational level but these differences could not explain th

Degradation of toxaphene in aged and freshly contaminated soil

Degradation of toxaphene in soil from both newly contaminated (from Sweden) and aged spills (from Nicaragua) were studied. The newly contaminated soil contained approximately 11 mg kg(-1) toxaphene while the aged Nicaraguan soil contained approximately 100 mg kg-1. Degradation was studied in anaerobic bioreactors, some of which were supplied with lactic acid and others with Triton (R) X-114. In th

Mutation analysis of the NSD1 gene in patients with autism spectrum disorders and macrocephaly

Background: Sotos syndrome is an overgrowth syndrome characterized by macrocephaly, advanced bone age, characteristic facial features, and learning disabilities, caused by mutations or deletions of the NSD1 gene, located at 5q35. Sotos syndrome has been described in a number of patients with autism spectrum disorders, suggesting that NSD1 could be involved in other cases of autism and macrocephaly

Biskop och stad : aspekter av urbanisering och sociala rum i medeltidens Linköping

Popular Abstract in Swedish Syftet med avhandlingen är att studera och analysera en historisk epok och dess människor genom deras materiella kultur och hur de sociala relationerna har manifesterats i rummet. Som fokus fungerar den medeltida biskopsstaden Linköping, en plats som skiftat utseende, funktion och betydelse genom olika perioder, men hela tiden med starka ideologiska uttryck. Linköping Is it possible to understand a society and a certain place that is long since gone? The aim of this thesis is to study and analyse a historic period and its people through the remains of their material culture, the social relations and how these were materialized in an urban setting. The main subject of the empirical study, the medieval episcopal town of Linköping, has shifted out-look, function a

Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni, and its clinical application

Antibody response to Campylobacter jejuni/coli (CJC) was investigated, using an enzyme-linked immunosorbent assay, ELISA. With a mixture of lipopolysaccharide from two CJC strains as antigen in ELISA, all 24 tested rabbit anti-CJC sera showed high antibody levels. However, only 70% of sera from patients with Campylobacter enteritis demonstrated an antibody response against the combined LPS antigen

Effects of storage conditions on degradation of chlorophyll and emulsifying capacity of thylakoid powders produced by different drying methods

Thylakoid membranes isolated from spinach have previously been shown to inhibit lipase/co-lipase and prolong satiety in vivo. There is a need to develop thylakoid products that not only have the desired characteristics and functionality after processing, but also are stable and provide equivalent effect on appetite over the promised shelf life. The aim of the present study was therefore to evaluat

Improved specificity of ICA assays in the Fourth International Immunology of Diabetes Serum Exchange Workshop

The goal of the Fourth International Workshop for Standardization of ICA Measurements was to determine the specificity of ICA assays and their ability to distinguish between control sera (n = 57) and sera from IDDM-related individuals-representing relatives of IDDM patients (n = 21), healthy individuals who later developed IDDM (n = 8), or newly diagnosed IDDM patients (n = 23). Results from 28 la

Lecticans in Vascular Remodeling of the Pulmonary and Fetal Circulation : Implications for Children with End-Stage Heart Disease

Introduction: Elevated pulmonary vascular resistance (PVR) is observed in both physiological and pathological states. Pulmonary hypertension (PH) is a condition associated with significant morbidity and mortality in neonates and children and curative pharmacotherapy is lacking in most cases. In utero, a high PVR allows for blood to bypass the lungs through the open ductus arteriosus (DA). Vascular

Bias in published randomized trials that compare collagenase injection with percutaneous needle fasciotomy in the treatment of Dupuytren disease : a systematic review

• Purpose: Controversy exists regarding the comparative efficacy of collagenase injection and percutaneous needle fasciotomy in the treatment of Dupuytren contracture. The randomized controlled trials (RCTs) that have compared the two treatment methods have reported results mostly implying similar treatment efficacy, durability, and complications. We aimed to review these RCTs regarding methodical

Particle rotations and energy dissipation during mechanical compression of granular materials

We present new in-situ measurements of particle rotations and energy dissipation during compression of 3D packings of stiff, frictional particles. Two confined, uniaxial compression tests with different degrees of lateral confinement are discussed. X-ray computed tomography and 3D X-ray diffraction were combined to provide inter-particle forces, slip and roll distances, twist angles, and energy di

Stability of the frequent COPD exacerbator in the general population : A Danish nationwide register-based study

Exacerbation frequency is central in treatment strategies for chronic obstructive pulmonary disease. However, whether chronic obstructive pulmonary disease patients from the general population with frequent exacerbations continue to have frequent exacerbations over an extended period of time is currently unknown. In this study, we aimed to investigate the stability of the frequent exacerbator in a

Nationella forskarkurser - global urbanism 2018

Microsoft Word - Global Urbanism Proposal 2018 red TG fr AB Swedish National Program in Human Geography PhD Education        1  PhD Course:    Global Urbanism: Exploring the remaking of cities and regions      Course Organizers  The course is organized by the Department of Human Geography, Stockholm University, The  Stockholm Urban and Regional Research Environment (SURE), and the Department of Hu

https://www.keg.lu.se/sites/keg.lu.se/files/nationella_forskarkurser_-_global_urbanism_2018.pdf - 2026-05-30

Utbildningsplan master final 20191220 eng

Utbildningsplan Kandidatprogram i personal- och arbetslivsfrågor Faculty of Fine and Performing Arts Master of Fine Arts programme, 120 credits KAFKO, Konstnärligt masterprogram i fri konst, 120 högskolepoäng Second-cycle degree programme requiring previous university study / Program med akademiska förkunskapskrav och med slutlig examen på avancerad nivå Details of approval The syllabus was approv

https://www.khm.lu.se/en/sites/khm.lu.se.en/files/utbildningsplan_master_final_20191220_eng_.pdf - 2026-05-30

Disposable ionic liquid coating for headspace solid-phase microextraction of benzene, toluene, ethylbenzene. and xylenes in paints followed by gas chromatography-flame ionization detection

Solid-phase microextraction (SPME) with a disposable ionic liquid (IL) coating was developed for headspace extraction of benzene, toluene, ethylbenzene, and xylenes (BTEX) in paints. The SPME fiber was coated with IL prior to every extraction, then the analytes were extracted and desorbed on the injection port of gas chromatography, and finally the IL coating on the fiber was washed out with solve

Nanoclay-induced bacterial flocculation for infection confinement

Effects of size and charge of anionic nanoclays on their interactions with bacteria-mimicking lipid membranes, bacterial lipopolysaccharide (LPS), and Gram-negative bacteria were investigated using ellipsometry, dynamic light scattering, ζ-potential measurements, and confocal microscopy combined with Live/Dead staining. Based on particle size and charge density, three different anionic hectorite n

Evaluation and Hydrological Application of CMADS Reanalysis Precipitation Data against Four Satellite Precipitation Products in the Upper Huaihe River Basin, China

Satellite- and reanalysis-based precipitation products are important data source for precipitation, particularly in areas with a sparse gauge network. Here, five open-access precipitation products, including the newly released China Meteorological Assimilation Driving Datasets for the Soil and Water Assessment Tool (SWAT) model (CMADS) reanalysis dataset and four widely used bias-adjusted satellit

Quality of Research Projects in Medical Education – Does Extended Time Lead to Higher Quality?

The research projects produced in higher education are important not only for developing skills in critical appraisal in order to give students tools for working evidence-based but also as a measure of quality of higher education. When the Bologna Process was implemented at Lund University in Sweden, the courses in research projects were extended and are now performed at the basic level as well as