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Leaking roofs in Tunisia

Summary The leaking of water through roofs is a major problem in many parts of Tunisia. As a part of a collaboration between "École N: ionale d'Ingénieurs de Tunis" (E.N.I.T.) and Lund University, Institute of Science and Technology (L.N.T.H.), Sweden, a measuring method has been developed to compare the watertightness of different roofing membranes. With the method, three roof structues with diff

Mutations in GYPB exon 5 drive the S-s-U+(var) phenotype in persons of African descent: implications for transfusion

BACKGROUND: The S-s-U- phenotype in African Americans is due to a GYPB deletion, however the molecular basis for the S-s-U+var phenotype is poorly understood. Variable reactivity of S-s-U+var RBCs with monoclonal anti-He or by anti-U has been demonstrated, however the underlying molecular bases for this phenotype remain to be established. STUDY DESIGN AND METHODS: Hemagglutination was performed on

Ornithine metabolism along the female mouse nephron: localization of ornithine decarboxylase and ornithine aminotransferase

The fate of ornithine in the nephron of the female OF-1 Swiss mouse remains unknown. The aim of the present study was to identify the nephron segments containing the key enzymes involved in ornithine metabolism: ornithine decarboxylase (ODC) and ornithine aminotransferase (OAT). Viable tubules isolated by microdissection were incubated with [1-14C]ornithine to study the oxidative pathway. Other tu

Improvement and validation of RAPD in combination with PFGE analysis of Salmonella enterica ssp enterica serovar Senftenberg strains isolated from feed mills

In 1995 and 1996 a Swedish feed mill had problems due to a persistent contamination of Salmonella enterica spp. enterica serovar Senftenberg that was difficult to eliminate. Forty-eight strains isolated from the feed mill, together with unrelated strains included to evaluate the discriminatory power and reproducibility, were analysed by pulsed-field gel electrophoresis (PFGE). The source of contam

Non-stoichiometry defects and radiation hardness of lead tungstate crystals PbWO4

It has been stated many times that the formation of radiation infringements in PbWO4 is to a big extent stipulated by the non-stoichiometry defects of the crystals, arising in the process of their growth and annealing. To refine the idea of characteristics of the non-stoichiometry defects and their effect on the radiation hardness of PbWO4, the current study is aimed at the melt composition infrin

Change and Identity : Protestant English Interpretations of John Henry Newman's Secession, 1845–1864

This dissertation argues that the English Protestant interpretations between 1845 and 1864 of John Henry Newman’s secession were related to the notions which formed part of the British national identity. It demonstrates how various writers modelled their interpretations of Newman’s secession on the beliefs of British anti-Catholicism. By understanding the British national identity as a common cult

Genome characteristics of primary carcinomas, local recurrences, carcinomatoses, and liver metastases from colorectal cancer patients

BACKGROUND: Colorectal cancer (CRC) is one of the most common causes of cancer-related deaths in the Western world, and despite the fact that metastases are usually the ultimate cause of deaths, the knowledge of the genetics of advanced stages of this disease is limited. In order to identify potential genetic abnormalities underlying the development of local and distant metastases in CRC patients,

Effect of warmer climate on the depletion of snowcovered area in the Satluj basin in the western Himalayan region

The effect of warmer climate on the depletion of snow-covered area has been evaluated for the Satluj River basin located in the western Himalayan region in India. Snowmelt runoff contributes substantially to the annual streamflow of this river. In order to study the impact of three warming scenarios (T+1, T+2 and T+3degreesC), more than 160 new snow depletion curves were prepared for different ele

Infection of BALB/c A mice by spiral and coccoid forms of Helicobacter pylori

Helicobacter pylori exists in two different morphological forms, spiral and coccoid. This study demonstrated that both forms can infect BALB/c A mice. The animals were inoculated orally three times at 2-day intervals with 10(8) cfu of both spiral and coccoid forms of strain CCUG 17874 (NCTC 11637), strain 25 and strain 553/93. Infection was followed over a 30-week period by histological scoring of

A common origin of the 4143insA ADAMTS13 mutation

Severely deficient activity of the von Willebrand Factor (VWF) cleaving metalloprotease,ADAMTS 13, is associated with thrombotic thrombocytopenic purpura (TTP). The mutation spectrum ofADAMTS 13 is rather heterogeneous, and numerous mutations spread across the gene have been described in association with congenital TTP. The 4143insA mutation is unusual with respect to its geographic concentration.

Ursodeoxycholic acid differentially affects three types of sphingomyelinase in human colon cancer Caco 2 cells.

We investigated the effects of UDCA on sphingomyelinase (SMase) in Caco 2 cells cultured in monolayer and polarized conditions. Alkaline SMase activity was high in polarized cells whereas. acid and neutral SMase activities were high in monolayer cells. In polarized cells, UDCA increased alkaline SMase expression and caspase 3 activity but had no effect on acid and neutral SMases. In monolayer cell

Infrared polarization spectroscopy of CO2 at atmospheric pressure

Polarisation spectroscopy (PS) was used to probe CO2 gas concentration in a CO2/N-2 binary mixture at atmospheric pressure and ambient temperature. The CO2 molecules were probed by a direct laser excitation to an overtone and combination vibrational state. The tuneable narrow linewidth infrared laser radiation at 2 mum was obtained by Raman shifting of the output from a single-longitudinal-mode pu

Incidence of cardiovascular disease, cancer and death in postmenopausal women affirming use of hormone replacement therapy.

AIM: The goal of this study was to evaluate the incidence of myocardial infarction, cancer and death in relation to use of hormone replacement therapy (HRT). METHODS: Nine years' follow up of an urban cohort of peri-/postmenopausal women was undertaken. Local and national registers were used for retrieval of events. RESULTS: The incidence of myocardial infarction per 1.000 person-years in users an

Optically stimulated luminescence from Al2O3 : C irradiated with 10-60 MeV protons

We investigated the potential use of Al2O3:C for medical proton dosimetry. Detector crystals coupled to fiber-optic cables were irradiated in proton beams with energies from 10 to 60 MeV. The key finding is that the initial intensity of the optically stimulated luminescence (OSL) signal is energy independent for small detectors (<0.5mm) and relatively small doses (<0.3Gy). This feature is related

Efficient and inefficient aspects of residential energy behaviour: What are the policy instruments for change?

The empirical part of this study is based on a survey of 600 Swedish households and a number of interviews where questions about residential energy behaviour and possible policy instruments for change were raised. The study provides insight into current behavioural patterns and gives a bottom-up perspective on the realistic perspective potentials for change and ways to achieve them. Residential en

Constrained optimization of a preparative ion-exchange step for antibody purification

Today, the optimization of chromatographic separation is usually based on experimental work and rule of thumb. The process and analytical technology (PAT) initiative, of the US Food and Drug Administration, has provided the opportunity of using model-based approach when designing downstream processing of pharmaceutical substances. A nonlinear chromatography model was used in this study to optimize

Bacterial immobilization and remineralization of N at different growth rates and N concentrations

An experiment was designed to resolve two largely unaddressed questions about the turnover of N in soils. One is the influence of microbial growth rate on mobilization and remineralization of cellular N. The other is to what extent heterotrophic immobilization of NO3- is controlled by the soil concentration of NH4+. Bacteria were extracted from a deciduous forest soil and inoculated into an aqueou

Selective removal of 17 beta-estradiol at trace concentration using a molecularly imprinted polymer

A molecularly imprinted polymer (MIP) was synthesized with 17 beta-estradiol (E2) as template. It was then capable to recover this compound by 100 +/- 0.6% from a 2 mu g/L aqueous solution. By comparison, E2 recoveries of 77 +/- 5.2%, 87.1 +/- 2.3% and 19.1 +/- 7.8%, were achieved using a non-imprinted polymer (NIP) synthesized under the same conditions (but without template), a commercial C18 ext

In vitro availability of starch in heat-treated potatoes as related to genotype, weight and storage time

The objective of the study was to determine the influence of potato variety, weight and storage time after lifting on the glycaemic index (GI) and resistant starch (RS) content predicted from measurement of the rate and extent of in vitro starch hydrolysis, respectively. The potatoes were either boiled, or boiled and subjected to different heat-cycling conditions selected to promote retrogradation