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Partial alkalinity, as a measure of bicarbonate concentration, and total alkalinity, as a measure of the concentration of bicarbonate and volatile fatty acids, are useful parameters for monitoring anaerobic digestion processes. This paper reports a new method based on pH-indicators and spectrophotometric measurements for the monitoring of partial and total alkalinity. The method was used in an off
We have developed a new ReaxFF reactive force field to describe accurately reactions of hydrocarbons with vanadium oxide catalysts. The ReaxFF force field parameters have been fit to a large quantum mechanics (QM) training set containing over 700 structures and energetics related to bond dissociations, angle and dihedral distortions, and reactions between hydrocarbons and vanadium oxide clusters.
Antibody-based microarray is a novel technology with great potential within high-throughput proteomics. The process of designing high-performing antibody (protein) microarrays has, however, turned out to be a challenging process. In this study, we have developed further our human recombinant single-chain variable-fragment (scFv) antibody microarray methodology by addressing two crucial technologic
A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target
Macroporous monolithic hydrogels (cryogel monoliths; rods 12.5 mm x 7.1 mm diameter) are elastic, sponge-like materials with large (10-100 mu m), interconnected pores. Phenyl- and IMAC-(Me(II)-iminodiacetic acid)-cryogel monoliths were inserted into the open-ended wells of a standard 96-well plate, forming a system of 96 drainage-protected minicolumns, and were used in a parallel assay of hydropho
Abstract Aim: Compared to neutral globular proteins, neutral polysaccharides, such as dextran, pullulan and Ficoll, appear hyperpermeable across the glomerular filtration barrier. This has been attributed to an increased flexibility and/or asymmetry of polysaccharides. The present study investigates whether polysaccharides are hyperpermeable also across the continuous capillaries in the rat perito
Stable isotopes of nitrogen (delta(15) N) and carbon (delta C-13) were measured for Atlantic salmon Salmo salar and their intestinal cestode, Eubothrium crassum, sharing the same diet. Atlantic salmon muscle tissues were enriched in N-15 and depleted in C-13 compared to their prey (sprat Sprattles sprattus sprattus) and their intestinal cestode. There was no significant difference in delta N-15 or
A new mechanistic model of drug release during the lag phase from coated pellets undergoing cracking in the coating due to the hydrostatic pressure built up inside the pellet has been developed. The model describes dynamically all the main release processes occurring during the lag phase in pellets coated with a semi-permeable membrane, i.e. the influx of solvent driven by the difference in osmoti
The early marsh orchid, Dactylorhiza incarnata (L.) Soo s. l., grows in medium-rich to rich fens and marshes over much of Europe and parts of Asia. The species is highly polymorphic and different forms may grow together at the same site. In the present study, I tested the hypothesis that these forms represent different migrant populations that have colonized Scandinavia independently of each other
Chemical signals are common in most crustacean social interactions and are often perceived via chemosensory (olfactory) organs on the first antenna. Intermolt courtship behaviors and mating were investigated in size-matched pairs of intermolt European lobsters (Homarus gammarus) where the olfactory receptors of either the male or the female were lesioned with distilled water (olfactory ablation) o
Thirty-three Lactobacillus strains were tested as adjuncts in a cheese model system. Eighteen strains originated from cheese (nine Lactobacillus spp. and nine Lb. paracasei/casei) and 15 from human intestinal mucosa (11 Lb. rhamnosus; three Lb. paracasei; one Lb. plantarum). Model cheeses weighing 120 g were made of cheese grains from full-scale production of washed curd semi-hard cheese (Herrgard
Abstract is not available
BACKGROUND: Host-related and environment-related factors have been shown to play a role in the development of tuberculosis (TB), but few studies were carried out to identify their respective roles in resource-poor countries. METHODS: A multicentre case-control study was conducted in Guinee, Guinea Bissau, and The Gambia, from January 1999 to March 2001. Cases were newly detected smear positive TB
Pulmonary embolism (PE) can only be diagnosed with imaging techniques, which in practice is performed using ventilation/perfusion scintigraphy (V/P(SCAN)) or multidetector computed tomography of the pulmonary arteries (MDCT). The epidemiology, natural history, pathophysiology and clinical presentation of PE are briefly reviewed. The primary objective of Part 1 of the Task Group's report was to dev
Comparisons of release rates, duration in the field, and catch efficiency of polyethylene and cotton roll dispensers for the sex pheromones of sawflies (Hymenoptera: Diprionidae) were conducted. The release rates of the neodiprion sertifer(Geoffr.) and Diprion pini (L.) sex pheromones, the acetates of pentadecanol and (2S,3S,7S)-3,7-dimethyl (2S,3R,7R)-3,7-dimethyl--tridecanol from polyethylene di
BACKGROUND. in this retrospective data base study, the authors sought to estimate direct costs for bone fractures in women age 65 years with early-stage breast carcinoma and to compare those costs with treatment costs for bone fractures in older women without early-stage breast carcinoma. METHODS. Direct costs for bone fractures in patients with early-stage breast carcinoma, which consist of exces
