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Background We have designed one-tube 14-antibody 10-color flow cytometry (FCM) panel that would provide maximum information on lymphoid and myeloid cell subsets in bone marrow aspirates (BMA) from patients with cytopenia(s). Samples and Methods BMA from 8 normal donors, from 286 non-malignant hospital controls, 92 myelodysplastic syndromes (MDS), 47 myeloproliferative neoplasms (MPN), and from 14
In order to generate monoclonal antibodies (MAb) directed against the low molecular weight glycoprotein alpha 1-microglobulin, a BALB/c mouse was immunized with a mixture of human, guinea pig, rat and rabbit alpha 1-microglobulin homologues (multi-species immunization) and boosted several times. On day 194, the mouse splenocytes were fused to SP2/0 myeloma cells. The resulting hybridomas were scre
The newly described immunoglobulin G-binding streptococcal surface protein, protein G, was used to prepare and characterize rabbit antibodies. The antibodies were directed against rat hormone-sensitive lipase, the rate-limiting enzyme in the hydrolysis of the triacylglycerols stored in adipose tissue. Antiserum was obtained after two injections with 20 micrograms enzyme protein, and the immunoglob
BACKGROUND: Current guidelines recommend that comatose out-of-hospital cardiac arrest patients with ST-segment elevations (STEs) following return of spontaneous circulation (ROSC) should be referred for an acute coronary angiography. We sought to investigate the diagnostic value of the pre-hospital ROSC-ECG in predicting ST-elevation myocardial infarction (STEMI).METHOD: ROSC-ECGs of 145 comatose
The WHO Checklist for Safe Surgery has been in use in Swedish healthcare since 2009. Based on national and international experiences, we have developed the original checklist, with the aim to keep its strengths and remove the weaknesses. The most obvious difference is that each section of the original checklist has been split into a pure checklist and corresponding instructions. It is now also mor
Economic behavior highlights the importance of differences in individual characteristics like cognition and emotional regulation strategy in predicting the selection and performance of economic choices. However, the literature on value premium has overlooked the effect of cognition and emotional regulation strategy in assessing individual preference to select value versus growth stocks. We fill th
RNA sequencing (RNAseq) methodology has experienced a burst of technological developments in the last decade, which has opened up opportunities for studying the mechanisms of adaptation to environmental factors at both the organismal and cellular level. Selecting the most suitable experimental approach for specific research questions and model systems can, however, be a challenge and researchers i
This paper presents a novel method to access lived religion and magical practices of a Medieval congregation via sermons combined with material culture. Previously, scholars have dismissed sermons as having low ‘truth value’ due to the copying inherent in the genre. In this paper, I first examine how one Danish sermon was adapted from a German model to fit a local context. This adaptation reveals
Biomass residues are often considered key in a reorientation towards circular bioeconomies, both by returning organic matter and nutrients to soils and by expanding the feedstock base for fossil-free products. Different indicators are available to assess progress towards circularity, but many available indicators and assessments seem to focus on product or material circularity, and lack in coverag
In Sweden, urban planners are legally obligated to design neighbourhoods that allow future buildings to meet daylight requirements. However, this is challenging because planners can only define the building masses, without control over future building details such as glazing ratios, room layouts, and orientation.The Vertical Sky Component (VSC) is a well-established indicator for early daylight as
A critical challenge in developing anion exchange membrane (AEM) water electrolysis is to design high-performance AEMs with chemical and mechanical stability under harsh alkaline environments. Herein, we report the rational design and synthesis of QP(T-3-Pip) featuring flexibly linked isomeric piperidinium cations via a Friedel-Crafts polyhydroxyalkylation between terphenyl and commercial 3-piperi
Background: The purpose of this analysis was to revisit the open US Food and Drug Administration (FDA) inspection list results from clinical trials and show the possibilities of evaluating inspection results between different world regions and explore what aspects of clinical trial deficiencies are most often reported.Aims: The aim of this study was to evaluate inspection results from different re
Polyurethane (PU) coatings are used in many industrial applications, like in the furniture and automotive sectors. The main objective of the present work is the re-design of polyester binder for PU coatings using a selection of monomers derived from biorefinery. A preliminary comparative evaluation of technological performances of the corresponding PU coatings is presented, showing that the introd
Two in situ 'nanoreactors' for high-resolution imaging of catalysts have been designed and applied at the hard X-ray nanoprobe endstation at beamline P06 of the PETRA III synchrotron radiation source. The reactors house samples supported on commercial MEMS chips, and were applied for complementary hard X-ray ptychography (23 nm spatial resolution) and transmission electron microscopy, with additio
The divide between interest groups and social movement studies runs deep, but present developments call for a renewed focus on the relevance of these analytical categories. Both of these two forms of collective action relate to organisations that are assumed to follow distinctive logics and strategies for political influence. This article aims to contribute to the debates on the analytical differe
The main goals of SI are usually to improve learning and student performance as well as increasing retention. The achievement of these goals are evaluated based on linking SI attendance with retention and student results (student performance is here often seen as a proxy for learning). However, the objectives with introducing an SI-program at a higher education institute is often so much more than
Peanut (Arachishypogaea L.) is one of the most important oil crops worldwide, and its yet increasing market demand may be met by genetic improvement of yield related traits, which may be facilitated by a good understanding of the underlying genetic base of these traits. Here, we have carried out a genome-wide association study (GWAS) with the aim to identify genomic regions and the candidate genes
