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The composition of the dominating population of freshly cut beef, and beef stored at 4 degrees C for 8 d, was studied by direct analysis of the 16S rRNA gene (PCR amplification, cloning and sequencing) and compared with pure culture technique where the isolates picked from the viable plate count were identified by sequencing of the 16S rRNA gene. The composition of the bacterial population was rec
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Chinese hamster ovary (CHO) cells in the plateau phase were seeded in the absence or presence of 5 mM 2-difluoromethylornithine (F(2)MeOrn), an enzyme-activated irreversible inhibitor of ornithine decarboxylase. The thymidine analogue bromodeoxyuridine (BrdUrd, 5 mu M) was added to the culture medium 30 min before sampling of the cells, which occurred 1-17 h after seeding. Using flow cytometry, co
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Objective: To examine the test-retest repeatability of pressure pain thresholds in the shoulder muscles of healthy women. Design: Four experimental sessions (days 1, 3, 28, 30), each including 4 consecutive pressure pain threshold measurements at 10-minute intervals. Subjects: Twenty-four healthy female volunteers, mean age 42 years. Methods: Two examiners measured pressure pain thresholds bilater
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Several authors have reported promising results with vacuum-assisted closure therapy in poststernotomy mediastinitis. The aim of this study was to investigate the hemodynamic outcome following the application of six negative pressures on an open sternotomy wound. Six 70-kg pigs underwent median sternotomy followed by vacuum-assisted closure therapy. Six negative pressures (-50, -75, -100, -125, -1
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A shear-enhanced, cross-flow ultrafiltration module was used to separate cells and proteins from the fermentation broth. Three (fermented) media were studied: rich medium, rich medium with hydrolytic enzymes added after fermentation, and wheat flour hydrolysate. To find a membrane with as high a flux as possible, but still capable of separating cells and proteins from the lactic acid containing br
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The significance of load modeling for voltage stability studies has been emphasized by several disturbances, which have taken place in the past years. They have shown that the loads in combination with other dynamics are among the main contributors of prolonged low voltage conditions, voltage instability and collapse in the power system. As a result of these disturbances new investigations have co
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Degradation of organic matter takes place in many processes within a wastewater treatment plant (WWTP). The need for optimisations and extended degradation processes for both organics and nitrogen will increase as outlet demands are strengthened and increasing loads are desired. The purpose of this work was to study the degradation of organic matter at WWTPs within two areas in particular; 1) d
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Most nuclear medicine clinicians use only visual assessment when interpreting regional cerebral blood flow (rCBF) from single-photon emission computed tomography (SPECT) images in clinical practice. The aims of this study were to develop a new, easy to use, automated method for quantification of rCBF-SPECT and to create normal values by using the method on a normal population. We developed a 3-dim
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For hemophilia patients with inhibitors, immune tolerance induction (ITI) may help to restore clinical response to Factor (F) VIII or FIX concentrates. Several ITI regimens and protocols exist; however, despite 30 yr of progressive investigation, the ITI evidence base relies mainly on observational data. Expert opinion, experience, and interpretation of the available evidence are therefore valuabl
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Background: A large number of individuals with Alzheimer’s disease (AD) live alone and receive little or no help from family members, which implies an additional pressure on the increasing societal costs of dementia care. Their adult children, if any, might have very limited ability to assist by providing informal help because of social structural changes, such as full-time work for most women and
Collecting payment from conference participants
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1. In the rat hepatic artery, the SK(Ca) inhibitors UCL 1684 (300 nM) completely blocked, and scyllatoxin (1 μM) and d-tubocurarine (100 μM) partially inhibited EDHF relaxations when each of them was combined with charybdotoxin (300 nM). 2. The IK(Ca) inhibitors clotrimazole (3 μM) and 2-chlorophenyl-bisphenyl-methanol (3 μM) strongly depressed EDHF relaxations when each of them was combined with
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Through a unique combination of time-resolved single-molecule (cryo-TEM) and bulk measurements (light scattering and small-angle X-ray scattering), we provide a detailed study of the dynamics of stochastic DNA ejection events from phage λ. We reveal that both binding with the specific phage receptor, LamB, and thermo-mechanical destabilization of the portal vertex on the capsid are required for in
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Shaken baby syndrome has typically been associated with findings of subdural haematoma, retinal haemorrhages and encephalopathy, which are referred to as the triad. During the last decade, however, the certainty with which the triad can indicate that an infant has been violently shaken has been increasingly questioned. The aim of this study was to determine the diagnostic accuracy of the triad in
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Observations of sea level and precipitation in Malmö, Sweden in the southeastern part of the sound Öresund have been used to estimate the probabilities of local compound events of high sea level and large daily and hourly rains. There are observations of sea level and daily rains extending back to 1930. The observations of short-term rainfall are from 1980 and onwards. Most large rainfalls come in
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BACKGROUND: In locally advanced rectal cancer, 5-Fluorouracil (5-FU)-based chemoradiation is the standard treatment. The main acute toxicity of this treatment is enteritis. Due to its potential radiosensitizing properties, oxaliplatin has recently been incorporated in many clinical chemoradiation protocols. The aim of this study was to investigate to what extent 5-FU and oxaliplatin influence the
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ObjectivesThe aims of the study were to investigate the prevalence of impaired sensation after minor salivary gland biopsy (MSGB) in two Swedish centres [Karolinska University Hospital (KUH) and Skåne University Hospital (SUH)] and to assess its impact on quality of life (QoL) and associated risk factors.MethodA questionnaire including questions regarding the presence of impaired sensation, impact
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Study region: In the Yangtze River basin of China. Study focus: We applied a recently popular deep learning (DL) algorithm, Transformer (TSF), and two commonly used DL methods, Long-Short-Term Memory (LSTM) and Gated Recurrent Unit (GRU), to evaluate the performance of TSF in predicting runoff in the Yangtze River basin. We also add the main structure of TSF, Self-Attention (SA), to the LSTM and G
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Microbial stoichiometry has become a key aspect in ecological research as shifts in microbial C:N, C:P and N:P ratios upon nutrient addition are presumed to give insight into relative nutrient limitations for soil microorganisms–with far-reaching implications for biogeochemical processes. However, this expectation has never been tested against direct methods of microbial growth responses to nutrie
