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As the population of the world increases, there will be a larger number of people with major neurocognitive disorder and anemerging need for prompt diagnosis and treatment. A digital cognitive test could lead to that more people can be tested withoutburdening healthcare. To explore elderly’s experiences of using a digital cognitive test a cognitive healthy population of 70-year-oldswere recruited
This paper presents a method for the design of nonuniform DFT filter banks for subband beamforming. Filter banks designed with the method are evaluated in subband beamforming in a real-world microphone array application. Different source positions in array applications give rise to different signal delays, which means that adaptive beamformers in the subbands alter the phase information of the sub
A label-free immunosensor for the direct detection of cholera toxin (CT) at sub-attomolar level has been developed based on potential-step capacitance measurements. Anti-CT antibody was adsorbed on gold nanoparticles (AuNPs) incorporated on a polytyramine-modified gold electrode. The concentration of CT was determined by detecting the change of capacitance caused by the formation of antibody-antig
In organisms encountering predictable environments, fixed development is expected, whereas in organisms that cannot predict their future environment, phenotypic plasticity would be optimal to increase local adaptation. To test this prediction we experimentally compared phenotypic plasticity in two rockyshore snail species; Littorina saxatilis releasing miniature snails on the shore, and Littorina
Generation of clusters of free eosinophil granules (Cfegs), through lysis of eosinophils, has recently been proposed as a major paradigm for ultimate activation of airway mucosal eosinophils. In the present study involving patients with seasonal allergic rhinitis, we have investigated whether generation of Cfegs in the nasal mucosa is a feature of allergic rhinitis. Nasal mucosal biopsies were obt
Polyamines added extracellularly to intestinal and vascular smooth muscle cells cause relaxation through inhibition of Ca2+ channel activity. Intracellularly applied polyamines also affect Ca2+ channel properties. Polyamines do not readily pass over the plasma membrane because of their positive charges but in permeabilized smooth muscle preparations they have free access to the cytoplasm. In this
The gain of neurotoxic function in amyotrophic lateral sclerosis (ALS) has been linked to misfolding of the homodimeric enzyme Cu/Zn superoxide dismutase (SOD). Here, we present the crystal structure of fully cysteine-depleted human SOD (SOD (CallA)), representing a reduced, marginally stable intermediate on the folding pathway in vivo that has also been implicated as neurotoxic precursor state. A
We investigated the effects of cell-free filtrates of the cyanobacteria Nodularia spumigena, Aphanizomenon sp. and Anabaena sp. on a brackish-water plankton community (< 150 mu m). In a laboratory experiment, we monitored the concentration of chlorophyll a and cell numbers of bacteria, phytoplankton and ciliates in the experimental units treated with cyanobacterial filtrates and the control medium
Chronic infection perturbs immune homeostasis. While prior studies have reported dysregulation of effector and memory cells, little is known about the effects on naïve T cell populations. We performed a cross-sectional study of chronic hepatitis C (cHCV) patients using tetramer-associated magnetic enrichment to study antigen-specific inexperienced CD8(+) T cells (i.e., tumor or unrelated virus-spe
Monte Carlo simulation is frequently employed to compute p-values for test statistics with unknown null distributions. However, the computations can be exceedingly time-consuming, and, in such cases, the use of pre-computed simulations can be considered to increase speed. This approach is attractive in principle, but complicated in practice because the size of the pre-computed data can be prohibit
Radome diagnostics are acquired in the design process, the delivery control, and in performance verification of repaired and newly developed radomes. A measured near or far field may indicate deviations, e.g., increased side-lobe levels, but the origins of the flaws are not revealed. In this paper, radome diagnostics is performed by visualizing the equivalent surface currents on the 3D-radome body
A label free biosensor for direct detection of inorganic phosphate based on potential-step capacitance measurements has been developed. The high-affinity Pho84 plasma membrane phosphate/proton symporter of Saccharomyces cerevisiae was used as a sensing element. Heterologously expressed and purified Pho84 protein was immobilized on a self-assembled monolayer (SAM) on a capacitance electrode. Change
The Centre party weathered what amounted to an almost existential mid-term public opinion crisis, to end up outperforming its coalition partners in the 2014 general election. Although it too fell back, it shed a notable proportion of tactical support voters, and actually increased its share of genuine, first-preference, voters. The Centre party has revisited and refreshed its ideological foundatio
Wall collision broadening of absorption lines of gases confined in porous media is a recently opened domain of high-resolution spectroscopy. Here, we present an experimental investigation of its application for pore size assessment. We report on the manufacturing of nanoporous zirconia ceramics with well-defined pore sizes fine-tuned from 50 to 150 nm. The resulting pore structure is characterized
In the growing field of nanotechnology there is an increasing need to develop production methods for nanoparticles, especially methods that provide control and reproducibility. The spark discharge generator (SDG) is a versatile device for the production of nanoparticle aerosols. It can produce aerosol nanoparticles in the entire nanometer range (1-100 nm), and beyond. Depending on requirements, an
X-ray spectroscopy studies of potassium intercalated metal-free phthalocyanine multilayers adsorbed on Al(110) have been undertaken. Photoelectron spectroscopy measurements show the presence of several charge states of the molecules upon K intercalation, due to a charge transfer from the alkali. In addition, the comparison of valence band photoemission spectra with the density functional theory ca
Single-molecule pulling experiments on unstructured proteins linked to neurodegenerative diseases have measured rupture forces comparable to those for stable folded proteins. To investigate the structural mechanisms of this unexpected force resistance, we perform pulling simulations of the amyloid β-peptide (Aβ) and α-synuclein (αS), starting from simulated conformational ensembles for the free mo
In the mid 2000s the oil and gas industry was hit by what might be best described as a 'wall of cash' as oil prices successively reached new record levels and access to external financing improved greatly. In this article we investigate what this sudden abundance of liquidity implied for the investment-cash flow relationship, the interpretation of which continues to generate controversy in the lit
FKBPs (FK506-binding proteins) have long been recognized as key regulators of the response to immunosuppressant drugs and as co-chaperones of steroid receptor complexes. More recently, evidence has emerged suggesting that this diverse protein family may also represent cancer biomarkers owing to their roles in cancer progression and response to treatment. FKBPL (FKBP-like) is a novel FKBP with role
