Filtyp
Wastewater-based surveillance (WBS) allows the analysis of pathogens, chemicals or other biomarkers in wastewater to derive unbiased epidemiological information at population scale. After re-gaining attention during the SARS-CoV-2 pandemic, the field holds promise as a surveillance and early warning system by tracking emerging pathogens with pandemic potential. Expanding the current toolbox of ana
Plant cells integrate signals from external sources and from organelles to regulate gene expression, referred to as anterograde and retrograde signaling, respectively. Functional characterization of the promoter of ALTERNATIVE OXIDASE1a (AOX1a) from Arabidopsis (Arabidopsis thaliana), a marker for mitochondrial retrograde response, was carried out by testing the ability of the AOX1a promoter to dr
Previous studies show that most birds inhabiting temperate regions have well defined life history stages, and they modulate the production of testosterone (T) and corticosterone (CORT) in response to changes in seasonality. In this study we aimed to examine baseline and stress-induced levels of CORT and circulating T in relation with life history stages in the rufous-collared sparrow, Zonotrichia
Exceptionally preserved fossil biotas of the Burgess Shale and a handful of other similar Cambrian deposits provide rare but critical insights into the early diversification of animals. The extraordinary preservation of labile tissues in these geographically widespread but temporally restricted soft-bodied fossil assemblages has remained enigmatic since Walcott's initial discovery in 1909. Here, w
Background-Sphingosine-1-phosphate (S1P) signaling is a central regulator of resistance artery tone. Therefore, S1P levels need to be tightly controlled through the delicate interplay of its generating enzyme sphingosine kinase 1 and its functional antagonist S1P phosphohydrolase-1. The intracellular localization of S1P phosphohydrolase-1 necessitates the import of extracellular S1P into the intra
Within insect communities, the population ecology of organisms representing higher trophic levels, for example, hymenopterous parasitoids, may be influenced by the structure of their insect hosts. Using microsatellite markers and ecological data, we investigated the population structure of the specialist braconid wasp parasitoid, Lysiphlebus hirticornis Mackauer attacking Metopeurum fuscoviride, a
Reactions of 4-thio-2′-deoxyuridine d(s4U) and 4-thiouridine 5′-monophosphate s4UMP with trans-[AuY2Cl2]+/– (Y = CN– or NH3) have been investigated by conventional and high-pressure stopped-flow spectrophotometry in aqueous solution with 2.9 ⩽ pH ⩽ 7.0. For a given combination of metal complex and sulfur donor the observed second-order rate constants decreased with increasing pH. In the pH range 2
This thesis explores how the tumour microenvironment affects the phenotype and shapes the evolution of cancer cells. It encompasses four separate studies:First, we explored the effect of chemokines on the peritotumoral microenvironment of ovarian cancer. We found that C-C Motif Chemokine Ligand 2 (CCL2) secreted from mesothelial cells is a dominant chemokine promoting the peritoneal dissemination
Aim: To assess growth plate fusion by magnetic resonance imaging (MRI) and evaluate the correlation with sex, age, pubertal development, physical activity and BMI. Methods: Wrist, knee and ankle of 958 healthy subjects aged 14.0-21.5 years old were examined using MRI and graded by two radiologists. Correlations of growth plate fusion score with age, pubertal development, physical activity and BMI
Biopharmaceuticals are a fast-growing niche in the pharmaceutical industry compared to small molecules. Even though a lot of progress has been made in recent years, the process to take a drug candidate to clinical trials can be lengthy and the selection process of high producing stable clones can be an arduous process. In this master thesis the design of new expression vectors for the production
Agricultural intensification is seen as a major threat to global biodiversity. Semi-natural grasslands play an important role in maintaining biodiversity in agricultural landscapes. Biodiversity and community structure in semi-natural grasslands depends not only on local management but also on the surrounding land-use. Further knowledge on landscape-scale effects would help improving the managemen
The Swedish Government announced that coming fall of 2022 the school curriculum will change significantly in its approach to sexual education to include subjects such as consent, sexuality and relationships, as well as critical conversations about representation of sex in different forms of media, including pornography. To combat children's exposure to porn, child rights activists are calling
Previous studies have shown that several forms of the glycoprotein tenascin are present in the embryonic extracellular matrix. These forms are the result of alternative splicing, which generates tenascin variants with different numbers of fibronectin type III repeats. We have used degenerate primers and PCR to isolate a novel tenascin exon from an avian genomic library. Genomic clones contained a
The extracellular matrix protein, tenascin, appears in a restricted pattern during organ morphogenesis. Tenascin accumulates along developing peripheral nerves as they leave the spinal cord and enter the limb mesenchyme (Wehrle and Chiquet, Development 110, 401-415, 1990). Here we found that most but not all tenascin deposited along growing nerves is of glial origin. By in situ hybridization with
In this study we demonstrate that, in addition to blood, alpha1-microglobulin (alpha1m) is present in most tissues, including liver, heart, eye, kidney, lung, pancreas, and skeletal muscle. Western blotting of perfused and homogenized rat tissue supernatants revealed alpha1m in its free, monomeric form and in high molecular weight forms, corresponding to the complexes fibronectin-alpha1m and alpha
The prevalence of anastomotic strictures in esophageal anastomoses provides us with limited information about the anastomotic healing process. This prospective study evaluates the exact esophageal anastomotic diameters in 256 patients who underwent esophagectomy and esophagogastrostomy without pyloroplasty (n = 107) or total gastrectomy and Roux reconstruction (n = 149). No perioperative chemoradi
Aeolian deposits in Sweden and Norway are poorly explored as environmental records. Many, mainly small, occurrences of windblown sand have been mapped, but few have been investigated in any detail and even fewer have been absolutely dated. Here we present results from a project aiming to determine the timing and magnitude of wind activity in inland settings in southern Sweden and Norway and to bet
Insulin stimulates Na+,K+-ATPase activity and induces translocation of Na+,K+-ATPase molecules to the plasma membrane in skeletal muscle. We determined the molecular mechanism by which insulin regulates Na+,K+-ATPase in differentiated primary human skeletal muscle cells (HSMCs). Insulin action on Na +,K+-ATPase was dependent on ERK1/2 in HSMCs. Sequence analysis of Na+,K+-ATPase α-subunits reveale
