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De novo active sites for resurrected Precambrian enzymes

Active Site Hydrophobicity and the Convergent Evolution of Paraoxonase Activity in Structurally Divergent Enzymes : The Case of Serum Paraoxonase 1

Computational Study of the pK_a Values of Potential Catalytic Residues in the Active Site of Monoamine Oxidase B

Monoamine oxidase (MAO), which exists in two isozymic forms, MAO A and MAO B, is an important flavoenzyme responsible for the metabolism of amine neurotransmitters such as dopamine, serotonin, and norepinephrine. Despite extensive research effort, neither the catalytic nor the inhibition mechanisms of MAO have been completely understood. There has also been dispute with regard to the protonation s

Completion of registration of risk factor variables during telephone vs on-site follow-up after myocardial infarction : a nationwide observational study in 101 199 patients from contemporary clinical practice in Sweden

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

Admission Control Schemes Guaranteeing Customer QoS in Commercial Web Sites

Quinone reduction sites and the role of heme in succinate:quinone reductase. Studies in Bacillus subtilis and Paracoccus denitrificans

Interaction of serum amyloid A with human cystatin Cuidentification of binding sites

Serum amyloid A (SAA) is a multifunctional acute-phase protein whose natural role seems to be participation in many physiologic and pathological processes. Prolonged increased SAA level in a number of chronic inflammatory and neoplastic diseases gives rise to reactive systemic amyloid A amyloidosis, where the N-terminal 76-amino acid residue-long segment of SAA is deposited as amyloid fibrils. Rec

Comparison of the active-site design of molybdenum oxo-transfer enzymes by quantum mechanical calculations.

SITES AquaNet : An open infrastructure for mesocosm experiments with high frequency sensor monitoring across lakes

Substrate-bound outward-open structure of a Na⁺-coupled sialic acid symporter reveals a new Na⁺ site

Many pathogenic bacteria utilise sialic acids as an energy source or use them as an external coating to evade immune detection. As such, bacteria that colonise sialylated environments deploy specific transporters to mediate import of scavenged sialic acids. Here, we report a substrate-bound 1.95 Å resolution structure and subsequent characterisation of SiaT, a sialic acid transporter from Proteus

Analysis of particle size distribution changes between three measurement sites in Northern Scandinavia

Measured aerosol size distributions from three measurement stations and modeled air mass trajectory data were combined to study aerosol dynamics in the boreal forest zone in Northern Scandinavia. Three approaches were used: investigation of new particle formation events, analysis of air masses arriving from ocean to continent, and study of changes in the aerosol size distributions when air masses

Acoustic mapping of submerged stone age sites—A HALD approach

An Enzyme with High Catalytic Proficiency Utilizes Distal Site Substrate Binding Energy to Stabilize the Closed State but at the Expense of Substrate Inhibition

Environmental Metabarcoding Reveals Contrasting Belowground and Aboveground Fungal Communities from Poplar at a Hg Phytomanagement Site

Russian internet news sites, 2008–2018 : Fostering an informed citizenry?

Since Perestroika, Russian journalism has gone through a liberalization. However, the short lived apex of journalistic freedom that took place in the late 1980s and early 1990s was followed by a setback in particular since Putin’s accession to power in 2000. The situation for the freedom of the daily press has stagnated. Despite this, the findings based on qualitative text analysis of news article

Pentadecamer-binding proteins : Definition of two independent protein-binding sites needed for functional activity

A blood test to monitor bee health across a European network of agricultural sites of different land-use by MALDI BeeTyping mass spectrometry

There are substantial concerns about impaired honey bee health and colony losses due to several poorly understood factors. We used MALDI profiling (MALDI BeeTyping®) analysis to investigate how some environmental and management factors under field conditions across Europe affected the honey bee haemolymph peptidome (all peptides in the circulatory fluid), as a profile of molecular markers represen

The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and tRNALys isoacceptors in the P site

A Transparent Modelling Workflow for the Assessment of Surface Water-Groundwater Exchange Flux at a PCE Contaminated Site