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Selection of random RNA fragments as method for searching for a site of regulation of translation of E. coli streptomycin mRNA by ribosomal protein S7

In E. coli cells ribosomal small subunit biogenesis is regulated by RNA-protein interactions involving protein S7. S7 initiates the subunit assembly interacting with 16S rRNA. During shift-down of rRNA synthesis level, free S7 inhibits self-translation by interacting with 96 nucleotides long specific region of streptomycin (str) mRNA between cistrons S12 and S7 (intercistron). Many bacteria do not

Structure and dynamics of the active site of hen egg-white lysozyme from atomic resolution neutron crystallography

Hen egg-white lysozyme (HEWL) is a widely used model protein in crystallographic studies and its enzymatic mechanism has been extensively investigated for decades. Despite this, the interaction between the reaction intermediate and the catalytic Asp52, as well as the orientation of Asn44 and Asn46 side chains, remain ambiguous. Here, we report the crystal structures of perdeuterated HEWL and D2O b

Low Migratory Connectivity and Use of Multiple Non-Breeding Sites in American Golden-Plovers Breeding Across the Nearctic Tundra

Aim: Many populations of migratory birds are currently declining. Understanding space use throughout the entire annual cycle, as well as migratory connectivity (i.e., geographic linkage of individuals and populations across different stages of the annual cycle), can improve our ability to identify factors driving population declines and influencing extinction risk. The main objectives of our study

Spatiotemporal photon distribution control on active sites enables bio-inspired methane-to-methanol conversion

Direct catalytic conversion of methane to methanol offers a pathway for transforming a potent greenhouse gas into a portable clean liquid fuel, thereby mitigating carbon emissions and supporting sustainable energy. However, this process faces challenges from thermodynamically favorable methanol overoxidation. Here, we show that spatiotemporal regulation of photogenerated charge carriers on enginee

The regulation of factor Va activity by activated protein C -Importance of the individual activated protein C cleavage sites in factor Va

Popular Abstract in Swedish Blodcirkulationen fungerar i kroppen som ett transport system för proteiner, syre och andra metaboliter. Blodet har den svåra uppgiften att vara flytande och samtidigt kunna koagulera på ställen där kärlet går sönder. Störning i denna balans leder till sjukdomar som hjärtinfarkt och blodpropp eller motsatsen svåra blödningar. Det protein som jag har studerat ingår vid

Local Politics of Renewable Energy: Project Planning, Siting Conflicts and Citizen Participation

Renewable energy will play an important role in the transition to a sustainable energy system. With an increased maturity of renewable energy technologies, issues concerning implementation are becoming more important. The decisions and actions of municipalities and other local actors have a significant influence on the implementation of renewable energy. In this thesis challenges to implementation