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Background: L1 is an important primary rRNA-binding protein, as well as a translational repressor that binds mRNA. It was shown that L1 proteins from some bacteria and archaea are functionally interchangeable within the ribosome and in the repression of translation. The crystal structure of bacterial L1 from Thermus thermophilus (TthL1) has previously been determined. Results: We report here the
Alcohols, such as 2,2,2-trifluoroethanol (TFE), have been shown to induce a cooperative transition to an open helical structure in many proteins, but the underlying molecular mechanism has not been identified. Here, we employ the technique of magnetic relaxation dispersion (MRD) to study the TFE-induced transition of -lactoglobulin at pH 2.4. Unlike traditional techniques that focus on protein sec
OBJECTIVE: ACAT2 is thought to be responsible for cholesteryl ester production in chylomicron and VLDL assembly. Recently, we identified HNF1alpha as an important regulator of the human ACAT2 promoter. Thus, we hypothesized that MODY3 (HNF1alpha gene mutations) and possibly MODY1 (HNF4alpha, upstream regulator of HNF1alpha, gene mutations) subjects may have lower VLDL esterified cholesterol. METHO
Background Organic acid anhydrides are low molecular weight industrial chemicals, able to cause rhinoconjunctivitis and asthma associated with specific IgE against hapten-carrier protein conjugate. Only a proportion of exposed workers develop IgE-associated allergy to acid anhydrides. Objective We determined whether genetic susceptibility, in particular, HLA Class II alleles may be a risk factor.
We report the observation of a spectroscopically resolved delayed electrofluorescence (DEF) in the time domain of nanosecond to microsecond (depending on temperature, in the range of 30-290 K, as well as bias) from light-emitting diodes based on poly[2-methoxy-5-(2(')-ethyl-hexyloxy)-1,4-phenylene vinylene]. The decay kinetics of this DEF are always found to be biexponential in nature. The fast de
The aim of this study was to compare the performance of the enzymes produced by Trichoderma reesei Rut C30 and the good extracellular beta-glucosidase-producing mutant Trichoderma atroviride TUB F-1663 to that of commercial preparations in the enzymatic hydrolysis and the simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce (SPS). The concentrated TUB F-1663 enzyme was f
Total oxidation of propane with oxygen in the absence and the respective presence of SO2 in the feed gas was studied over 1 wt% Pt on gamma -Al2O3 using transient experiments with TAP (temporal analysis of products) and in situ DRIFT spectroscopy. The TAP experiments confirm the promoting role of SO2 on the conversion of propane and, moreover, show an inhibiting effect from SO2 on the conversion o
The work done during the past decade in order to adapt molecularly imprinted polymers (MIPs) to the capillary format and subsequently use these highly selective matrices for capillary electrochromatography (CEC) are reviewed in this article. MIPs are prepared utilizing a templated polymer synthesis where the template addresses the selectivity of the resulting polymer. These polymers possess bindin
Information on soil mineralogy is essential for weathering rate calculations. Quantitative mineralogical analyses are expensive and time-consuming, and thus indirect methods of determining the mineralogical composition are important, for example estimating the composition based on the elemental content in till (total concentrations), or based on information about the underlying bedrock. The minera
BACKGROUND: The S-s-U- phenotype in African Americans is due to a GYPB deletion, however the molecular basis for the S-s-U+var phenotype is poorly understood. Variable reactivity of S-s-U+var RBCs with monoclonal anti-He or by anti-U has been demonstrated, however the underlying molecular bases for this phenotype remain to be established. STUDY DESIGN AND METHODS: Hemagglutination was performed on
Glycine receptors (GlyRs) and specific subtypes of GABA(A) receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain
The surface properties of a protein are often crucial for recognition and interaction with other molecules. Important functional residues can be identified by mutational analysis. There is a need for rapid methods to study protein surfaces and surface changes due to mutations. Partitioning in aqueous two-phase systems has the potential to be used in this respect since protein partitioning depends
This ergonomics exposure assessment study compared the efficiency of eight 1-day-only strategies, that is, the relationship between the number of data collected per subject and the precision of the resulting mean exposure estimate. Whole-day electromyographic recordings from the right upper trapezius muscle in 24 cleaners and 23 office workers were processed to give minute-by-minute values of gap
