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Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

Kan hemoglobin från växter lösa humana problem? Dagligen tar vi för givet att vårt blod syresätts tillräckligt för att orka med alla sysslor. Detta möjliggörs av det livsviktiga proteinet hemoglobin som finns i vårt blod. I dagens samhälle råder det dock blodbrist samtidigt som många drabbas av järnbrist. Det är där forskningen på växthemoglobin kommer in i bilden. Experimenten som genomförts i dThe protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. A few years ago, genes that code for Pgb in sugar beet were found and ident

Building capacity to adapt to climate change through local actor support : a case study of an informal settlement in Rio de Janeiro, Brazil

Climate change is expected to increase the frequency and intensity of the most severe weather-related hazards over the coming decades. The poor in developing countries are particularly susceptible to climate change, often living in informal or precarious settlements. In order to reduce the vulnerability in those areas and strengthen people´s capacities to adapt to climate change, there is a need f

Traditional and Digital Building Archaeology, a Balance Between Old and New: A case study of the abbey Saint-Mathieu de Fine-Terre

This thesis aimed to work with different techniques of building archaeology, both traditional and digital. The goal was to define the possibilities offered by the “traditional” and the “digital” building archaeology but also to show the limits of these two methods. The main issue of discussing methodological problems in building archaeology was the need to define digital building archaeology, espe

The development of non-binary gender identities – an example from the muxes of Juchitán de Zaragoza, Oaxaca, Mexico

The growing complexity of the concept of gender highlights the importance for a deeper understanding of the diversity underlying this construct. Recent studies have shown large numbers of individuals experiencing non-conforming feelings with their sex assigned at birth, emphasising the need to gain a better understanding of gender identities outside the binary system, one of these being non-binary

Stratigraphic studies of the Taniente Palet bog at the north-western corner of Isla de los Estados Tierra del Fuego, South America

Fjorton, något överlappande, en meter långa borrkärnor har tagits från mossen Taniente Palet bog på ön Isla de los Estados, belägen i Tierra del Fuego i Argentina. De fjorton borrkärnorna bildar tillsammans en hel sekvens på ungefär 10.90 meter. Borrkärnorna har beskrivits och korrelerats, både visuellt och med hjälp av magnetisk susceptibilitet och glödförlust. Tolv prover har också tagits för ål

(De)legitimizing the Migration Court’s Judicial Decision: A Case Study on Social Media Discourses

This study aims to explore how the Swedish Migration Court’s decision is legitimized or delegitimized in discourses in civil society, outside the legislative and judicial power. This is explored with a case study on a judicial decision to expel Sonya, a 90-year-old and sick Ukrainian woman. The material is collected from social media platforms where this case has been discussed. Thus, the results

(De-)Stabilizing Europe’s Neighborhood? - An assessment of the EU’s Common Security and Defence Policy’s impact on host country stability

The EU’s Common Security and Defence Policy was created to stabilize the EU’s Neighbourhood, fuel the EU’s geopolitical crisis management ambitions, and strengthen European values abroad. In the recent debate, however, it was criticized as ineffective or destabilizing. While specific regions and mission types were previously researched, a comprehensive analysis of all EU crisis management operatio

De fontibus revelationis : the debate about a constitutive tradition during the Second Vatican Council

This investigation deals with the debate surrounding the controversial question of whether there exists a constitutive tradition at the Second Vatican Council (1962-1965). The investigation can broadly be divided into three sections. The first section provides both an analysis of the terminology relevant for understanding and a theological background. The background leads up the Council of Trent (

How to sow peace? : a value chain approach for environmental peacebuilding in Sierra de la Macarena National Park, Colombia

Ecological rehabilitation projects - as a form of reforestation interventions - are increasing worldwide and present an opportunity for climate change mitigation and supporting local livelihoods. Implemented in post-conflict situations, they are increasingly promoted as solutions for peace. Aiming at understanding their contributions to sustainability and peacebuilding, I examine proposed rehabili

Investigating the biogas potential from organic waste flows in Santiago de Chile – A study of the Metropolitan Region.

Matrester i Chiles huvudstad kan driva bussar och ge landet skjuts i klimatfrågan. Två tredjedelar av stadsbussarna i Santiago de Chile skulle kunna drivas av biogas, om det organiska avfallet i landets kärnregion tas tillvara på. Förändringen hade dessutom minskat Chiles totala klimatpåverkan med 2%. I Sverige är vi vana vid att åka stadsbuss där det glatt annonseras att den drivs av matavfalle

Characterisation of de novo mutations in the C-terminal domain of proprotein convertase subtilisin/kexin type 9.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes the degradation of the hepatic low-density lipoprotein receptor (LDL-R) and is therefore a prominent therapeutic target for reducing LDL-cholesterol. The C-terminal domain of PCSK9 is unlikely to be involved in a direct extracellular interaction with the LDL-R. We probed the importance of the C-terminus for the degradation of the LDL-R

Advokater och intressekonflikter- exemplet controlled auctions

Denna uppsats tar sin utgångspunkt i den livliga debatt som har förts inom advokatsamfundet sedan april 2004 angående intressekonflikter. Bakgrunden är den att advokatsamfundet utfärdade ett vägledande uttalande som förbjöd representation av flera klienter vid en viss typ av köpeprocess av företag, kallad controlled auctions. Advokatsamfundet och flertalet svenska advokater hävdade att detta bara