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Human antibodies generated in vitro may be highly valuable for diagnostic and therapeutic purposes. For a long time most human antibodies produced in vitro have been of the IgM isotype with rather low affinities. In this thesis a method for production of IgG switched antigen-specific human antibodies is presented. This is a two step protocol, in which the first step is a selection procedure of don
Artikel om bilden i konst- och historievetenskaplig forskning.
Nuklearmedicinsk bildtagning med hjälp av radiofarmaka är ett viktigt verktyg för att studera biologiska processer och humansjukdomar. Bildtagning sker ofta med en detektor i kombination med en kollimator. En typ av kollimering är pinnhålskollimering, där ett pinnhål i ett opakt material placeras mellan en källa och en detektor. Pinnhålet fyller samma funktion som en optisk lins då emitterade fotoThe focus of this dissertation is the use of pinhole collimation in Nuclear Medicine. A pinhole is a single aperture in an opaque material that is placed between the detector and source of interest, and photons must pass through the hole to reach the detector. The choices of pinhole parameters, e.g. the pinhole material and size of the opening, are closely linked to the performance of the detector
β-1,4-Galactosyltransferase 7 (β4GalT7) is a key enzyme in the biosynthesis of glycosaminoglycan (GAG) chains. Natural and synthetic xylosides can be used to both inhibit and prime GAG synthesis by acting as inhibitors or substrates for β4GalT7. In this report, we exploit hydroxylated oxanes as deoxygenated xyloside analogs to clarify the minimum protein-ligand interactions required for galactosyl
Background: Loss of chromosome 11q defines a subset of high-stage aggressive neuroblastomas. Deletions are typically large and mapping efforts have thus far not lead to a well defined consensus region, which hampers the identification of positional candidate tumour suppressor genes. In a previous study, functional evidence for a neuroblastoma suppressor gene on chromosome 11 was obtained through m
A new system for photon induced fluorescence spectroscopy (PIFS) at the gas phase photoemission beamline (Elettra, Trieste) is described. It consists of a novel non-dispersive detector for VUV photons and a spectrometer for UV/visible light with cooled CCD detector. To illustrate the performance of the apparatus, the fluorescence decay of the doubly excited states of helium below the N = 2 ionizat
The diagnosis of osteoporosis is based on the measurement of bone mineral density (BMD). There are a number of clinical risk factors that provide information on fracture risk over and above that given by BMD. The assessment of fracture risk thus needs to be distinguished from diagnosis to take account of the independent value of the clinical risk factors. These include age, a prior fragility fract
In this paper the first resonant photoemission and partial electron yield studies of free clusters are presented. The resonant photoemission spectra of free argon clusters have been recorded with excitation energies determined from the cluster X-ray absorption spectrum near the Ar 2p ionization threshold. The decay of 2p(-1)4s and 2p(-1)3d cluster surface and "bulk" excited states has been investi
Resonant and normal Auger electron spectra associated with an O1s initial vacancy of the O-2 molecule have been recorded using photon energies of 541.80, 541.97 and 650 eV. The resonant Auger spectra obtained at 541.80 and 541.97 eV are interpreted as composed essentially of two characteristic parts, one between 10 and 35 eV binding energy reminiscent to direct photoionisation, and another, above
Non-invasive characterization of brain structure has been made possible by the introduction of magnetic resonance imaging (MRI). Graph modeling of structural connectivity has been useful, but is often limited to defining nodes as regions from a brain atlas. Here, we propose two methods for encoding structural connectivity in a huge brain graph at the voxel-level resolution (i.e., 850'000 voxels) b
The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relationships between the pathogenic and mutualistic microorganisms of the honeybee microbiome, in particula
Aim: To determine the incidence of late intracranial vitamin K deficiency bleeding (VKDB) in The Netherlands using the Dutch Pediatric Intensive Care Evaluation (PICE) registry. Methods: The PICE registry was used to identify all infants who were admitted to a Dutch pediatric intensive care unit (PICU) with intracranial bleeding between 1 January 2004 and 31 December 2007. Cases of confirmed late
The disorders of serine biosynthesis are a group of inborn errors of metabolism characterised by congenital microcephaly, seizures and severe psychomotor retardation. Although these disorders are rare the prompt recognition of serine deficiency is important as these disorders are treatable. The diagnosis is based on decreased concentrations of serine in cerebrospinal fluid (CSF). It has previously
Definitive hematopoiesis emerges via an endothelial-to-hematopoietic transition in the embryo and placenta; however, the precursor cells to hemogenic endothelium are not defined phenotypically. We previously demonstrated that the induction of hematopoietic progenitors from fibroblasts progresses through hemogenic precursors that are Prom1+Sca1+CD34+CD45- (PS34CD45-). Guided by these studies, we an
In order to improve the selectivity in photocatalytic process, molecularly imprinted photocatalysts containing a low loading of TiO2 (from 6.6 to 16.6% of total mass) were prepared for photocatalytic degradation of an organic pollutant, diclofenac (DIC). The photocatalytic component TiO2 (P25), with and without being doped with Cu2O, was embedded in diclofenac-imprinted polymers. The molecularly i
Obesity is more common in individuals with mobility disability than in those without this condition. Individuals with mobility disability also have lower health-related quality of life (HRQoL) and are limited in their participation in society. Therefore, this study aimed to investigate the body mass index (BMI) status and the association of overweight or obesity on HRQoL and participation in socie
Advances in sequencing technologies led to rapid increase in the number and diversity of biological sequences, which facilitated development in the sequence research. In this paper, we present a new method for analyzing protein sequence similarity. We calculated the spectral radii of 20 amino acids (AAs) and put forward a novel 2-D graphical representation of protein sequences. To characterize pro
The resonant Auger spectrum from the decay of F 1s-excited CF4 is measured. Several lines exhibit a nondispersive kinetic energy as the exciting photon energy is tuned through the resonance region. The F 1s(-1) atomiclike Auger line is split into two components due to the emission of Auger electrons by a fragment in motion, when electron emission is observed along the polarization vector of the li
This study gives an outlook on the carbon balance of Sub-Saharan Africa (SSA) by presenting a summary of currently available results from the project CarboAfrica (namely net ecosystem productivity and emissions from fires, deforestation and forest degradation, by field and model estimates) supplemented by bibliographic data and compared with a new synthesis of the data from national communications
