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A novel and facile preparation method for spherical molecularly imprinted polymer (MIP) beads is presented. Two types of beads were synthesized and investigated: (i) silica-MIP composites were obtained by filling spherical, porous C-4-coated silica beads with print molecule and monomers followed by polymerisation; (ii) spherical molecularly imprinted polymer beads were acquired mirroring the silic
We report a flow-injection biosensor system with a capacitive transducer for assay and quality control of human immunoglobulin G (hIgG). The sensing platform is based on self-assembled monolayers (SAMs) of carboxylic acid terminated alkyl-thiols with covalently attached concanavalin A. The electrochemical characteristics of the sensor surface were assessed by cyclic voltammetry using a permeable r
The binding of Helicobacter pylori to glycosphingolipids was examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. In addition to previously reported binding specificities, a selective binding to a non-acid tetraglycosylceramide of human meconium was found. This H. pylori binding glycosphingolipid was isolated and, on the basis of mass spectrometry, proton
This study examined the role of P and type 1 fimbriae for neutrophil migration across Escherichia coli-infected uroepithelial cell layers in vitro and for neutrophil recruitment to the urinary tract in vivo. Recombinant E. coli K-12 strains differing in P or type 1 fimbrial expression were used to infect confluent epithelial layers on the underside of transwell inserts. Neutrophils were added to t
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of prot
Popular Abstract in Swedish Avhandlingen analyserar den schweiziske teologen Hans Küng och hans arbete med att utforma en universell moral. Enligt Küng kan världsreligionerna ligga till grund för en moral som kan sträcka sig över alla ideologiska, kulturella och religiösa gränser. Denna moral, som vilar på världsreligionerna, kan sedan ligga till grund för världspolitiken och garantera att relatioThis dissertation analyzes the work on universal morality by the Swiss theologian Hans Küng. According to Küng, a universal morality can be formed out of the world's religions that can reconcile ideological, cultural and religious differences. This morality can then form the basis for international policy and politics and guarantee that relations among states are peaceful. Küng's project focuses
Comparisons have revealed large discrepancies among the many methods for measuring soil CO2 efflux indicating the need for an absolute calibration of methods. This study presents a calibration system, constructed to imitate an area of soil, and its application to two different chamber systems for the measurement Of Soil CO2 efflux: one open and one closed dynamic. Air rich in CO2 was allowed to di
The presence of a specialised dorsal rim area with an ability to detect the e-vector orientation of polarised light is shown for the first time in a nocturnal hymenopteran. The dorsal rim area of the halictid bee Megalopta genalis features a number of characteristic anatomical specialisations including an increased rhabdom diameter and a lack of primary screening pigments. Optically, these special
Several strains belonging to genera Pseudomonas and Achromobacter and characterized by the ability to degrade anionic surfactants were tested as potential bases of microbial biosensors for surfactant detection. For each strain the substrate specificity and stability of sensor signals were studied. The total amount of the substrates tested (including carbohydrates, alcohols, aromatics, organic acid
We report on the applications of a single-longitudinal-mode (SLM) pulsed alexandrite laser system for diagnostics of parameters of flow/combustion interest. The laser system is characterized by its narrow linewidth, high peak power, and broad tunablity. The absolute frequency of the laser output was monitored by a wavelength diagnostic system, which included a high-resolution confocal etalon and a
Analytic expressions for the exact bit error probabilities of rate R = 1/2, memory m = 2 convolutional encoders are derived for a maximum-likelihood (ML) decoder and transmission over the binary-symmetric channel (BSC). The resulting expressions are rational functions of the crossover probability of the BSC. In addition to classical nonsystematic encoders without feedback, we consider also recursi
Combination chemotherapy may induce remission from acute myeloid leukemia (AML), but validated criteria for treatment of elderly are lacking. The remission intention ( RI) rate for elderly patients, as reported to the Swedish Leukemia Registry, was known to be different when comparing the six health care regions, but the consequences of different management are unknown. The Leukemia Registry, cont
Under seeing limited conditions very high resolution spectroscopy becomes very difficult for extremely large telescopes (ELTs). Using adaptive optics (AO) the stellar image size decreases proportional with the telescope diameter. This makes the spectrograph optics and hence its resolution independent of the telescope diameter. However AO for use with ELTs at visible wavelengths require deformable
We tested the hypothesis that populations in small habitat fragments remaining in agricultural landscapes are maintained by repeated immigration, using three grassland butterflies (Aphantopus hyperantus, Coenonympha pamphilus and Maniola jurtina). Transect counts in 12 matched sets of semi-natural pastures, and linear habitat elements proximate and isolated from the pastures showed that population
