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We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 an
Musculoskeletal conditions often manifest with the onset of pain and the resulting physical limitations. Musculoskeletal pain is almost inevitable in in individual's lifetime. It is one of the most common reasons for self-medication and entry into the health care system. Musculoskeletal pain affects in 4 adults and is the Most commons Source of serious long-term pain and physical disability. The M
We show that the spatial distribution of the electromagnetic (EM) field enhancement can be probed directly via dynamic evolution of surface-enhanced Raman scattering (SERS) of rhodamine 6G (R6G) molecules as they diffuse into Ag@SiO2 core-shell nanoparticles. The porous silica shell limits the diffusion of R6G molecules toward Inner Ag cores, thereby allowing direct observation and quantification
Although it is often assumed that birds strongly prefer tailwinds for their migratory flights, we predict that a strategy of no wind selectivity (traveling independently of winds) may be more favorable than wind selectivity (traveling on tailwind occasions but stopping to rest under headwind occasions) for birds with low energy costs of travel relative to rest and for birds that cannot use stopove
The number of regulatory RNAs with identified non-canonical structures is increasing, and structural transitions often play a role in their biological function. This stimulates interest in internal motions of RNA, which can underlie structural transitions. Heteronuclear NMR relaxation measurements, which are commonly used to study internal motion, only report on local motions of few sites within t
A Holocene palaeomagnetic secular variation master curve (FENNOSTACK) and a relative palaeointensity curve (FENNORPIS) for Fennoscandia are presented. These curves were produced by stacking palaeomagnetic data obtained from six annually laminated (varved) lake sediment sequences and one non-laminated sediment sequence. The six independent varve chronologies were combined to form a timescale, which
Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer
Background: This study examined the natural course of patients following surgery for small bowel obstruction (SBO) caused by abdominal adhesions. In addition, a cost analysis was performed. Methods: A retrospective analysis was undertaken of 102 patients who underwent surgery between 1987 and 1992 for intestinal obstruction due to abdominal adhesions. Results: Median follow-up was 14 years. The 10
This article documents new sightings of the leafcutting bee Megachile lagopoda (L.) in Åhus, Scania, Sweden on cotton thistle in a pine forest. The importance of areas of undeveloped land as habitat for rare species and the dispersal range of the bee are discussed.
Opera improvisation at The Malmö Academy of Music has been a pedagogical and artistic experiment. This is the final account concerning this pedagogical and artistic research project. Singers who are studying classical singing at the bachelor and master programmes of theacademy have participated. The account includes historical and theoretical reflections on the subject and comments on the work in
Popular Abstract in Swedish Denna doktorsavhandling är en delstudie i ett större projekt som syftar till att utveckla Artificiell fotosyntes för att framställa ett värdefullt bränsle, vätgas, från solenergi och vatten. Vår typ av Artificiell Fotosyntes syftar till att framställa syntetiska “superämnen” som kan härma nyckelsteg i den naturliga fotosyntesen. Våra artificiella ”superämnen” skall kunnIn natural photosynthesis, solar energy is converted to chemical energy by photosynthetic reaction centers. In green plants and algae, Photosystem II (PSII) and Photosystem I (PSI) absorb light and utilize the charge separation reactions to convert solar energy to chemical energy. The energy is later stored in form of energy- rich substances formed in reductive biosynthetic reactions. The electron
The purpose of this licentiate thesis is to investigate the following hypothesis in a case study: "Occupants of buildings with a quality labeled indoor environment experience a better indoor environment than occupants of buildings with normal planning and construction." The aim of the case study is to compare the outcome of using a quality system for indoor environment with a normal quality system
An automatic time sequence filter sampler has been developed for atmospheric aerosol particle sampling of ambient air and subsequent analysis for elemental composition using the proton beam of a Van de Graaff accelerator. Some features of the operation of the sampler are given, the retention of particles by the filter material are discussed, examples of the type of data obtained are presented, and
