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Purification and identification of the violaxanthin de-epoxidase as a 43 kDa protein

Violaxanthin deepoxidase (VDE) has been purified from spinach (Spinacia oleracea) leaves. The purification included differential sonication of thylakoid membranes, differential (NH4)2SO4 fractionation, gel filtration chromatography and finally either hydrophobic interaction chromatography or anion exchange chromatography. A total purification of more than 5000-fold compared to the original thylako

Virtual participatory mapping of nature-based solutions in the Grande de Tárcoles River basin, Costa Rica : Connecting diverse knowledge systems in a context of physical immobility

Co-design processes are of key importance in planning and implementing Nature-based Solutions (NBS), but require boundary management between diverse holders of local, practical and scientific knowledge. Participatory and mapbased planning tools, such as Geodesign workshops, can facilitate such boundary management through mobilising knowledge holders, and translating and negotiating between differe

(De)composing the middle : A minimalist approach to middles in English and Swedish.

This thesis is concerned with a particular type of generic sentence that is referred to as the ‘middle construction’ in the literature. The aim of the thesis is to offer a theoretical account of middles couched within the non-lexicalist framework of Distributed Morphology (see Halle & Marantz 1993 and subsequent work), and, more generally, within the Minimalist Program (see e.g. Chomsky 2000,