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Buddhism and Resilience in Post-tsunami Thailand

Religion is particularly important in times of crises and difficulties. After the 2004 Indian Ocean tsunami Buddhist monks became of great significance for the survivors in Thailand. They performed cremations, helped the survivors to understand and accept what has happened, provided leadership, conducted important rituals and organized aid. The Buddhist temples became the main venues for people se

Biased Inference of Selection Due to GC-Biased Gene Conversion and the Rate of Protein Evolution in Flycatchers When Accounting for It

The rate of recombination impacts on rates of protein evolution for at least two reasons: it affects the efficacy of selection due to linkage and influences sequence evolution through the process of GC-biased gene conversion (gBGC). We studied how recombination, via gBGC, affects inferences of selection in gene sequences using comparative genomic and population genomic data from the collared flyca

Chlorophyll biosynthesis in barley : Studies on the cyclase and chlorophyll synthase steps

The well-known green color of plants is due to the chlorophyll molecules used for light harvestingduring photosynthesis, which takes place in chloroplasts. Biosynthesis of chlorophyll a is performed by15 enzymatic steps and chlorophyll a can be interconverted to chlorophyll b via the chlorophyll cycle.Barley is a valuable model organism for studying chloroplast development and chlorophyll biosynth

van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture

Phosphate plays a crucial role in biology because of the stability of the phosphate ester bond. To overcome this inherent stability, enzymes that catalyze phosphoryl transfer reactions achieve enormous rate accelerations to operate on biologically relevant time scales, and the mechanisms that underpin catalysis have been the subject of extensive debate. In an archetypal system, β-phosphoglucomutas

Diagnostic accuracy for CZT gamma camera compared to conventional gamma camera technique with myocardial perfusion single-photon emission computed tomography : Assessment of myocardial infarction and function

Background: The solid-state cadmium-zinc-telluride (CZT) gamma camera for myocardial perfusion single-photon emission computed tomography (MPS) has theoretical advantages compared to the conventional gamma camera technique. This includes more sensitive detectors and better energy resolution. We aimed to explore the diagnostic performance of gated MPS with a CZT gamma camera compared to a conventio

Cost-effective sequence analysis of 113 genes in 1,192 probands with retinitis pigmentosa and Leber congenital amaurosis

Introduction: Retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA) are two groups of inherited retinal diseases (IRDs) where the rod photoreceptors degenerate followed by the cone photoreceptors of the retina. A genetic diagnosis for IRDs is challenging since >280 genes are associated with these conditions. While whole exome sequencing (WES) is commonly used by diagnostic facilities, the

Flexibility and Function of Distal Substrate-Binding Tryptophans in the Blue Mussel β-Mannanase MeMan5A and Their Role in Hydrolysis and Transglycosylation

β-Mannanases hydrolyze β-mannans, important components of plant and microalgae cell walls. Retaining β-mannanases can also catalyze transglycosylation, forming new β-mannosidic bonds that are applicable for synthesis. This study focused on the blue mussel (Mytilus edulis) GH5_10 β-mannanase MeMan5A, which contains two semi-conserved tryptophans (W240 and W281) in the distal subsite +2 of its activ

Nanocarbon oxidation in the environmental transmission electron microscope : Disentangling the role of the electron beam

Environmental transmission electron microscopy (ETEM) can provide unique insights into nanocarbon oxidation processes through atomic resolution and real time imaging of materials at high temperatures in reactive atmospheres. However, the electron beam can also influence the reaction rates, and even alter the processes entirely, complicating the interpretation of the in situ observations. Many mech

Soil, fertilizer and plant density : Exploring the influence of environmental factors to stable nitrogen and carbon isotope composition in cereal grain

Stable isotope analysis (N and C) of fossil cereal grains is regularly explored as a means of obtaining insights to past crop growing conditions and agricultural practices. In this study, we assessed how several growth conditions can affect δ15N and δ13C values of modern cereal grain with the aim to help understand isotopic values from ancient cereal remains. We investigated the impact of fertiliz

Carbon dioxide and methane fluxes from mounds of African fungus-growing termites

Termites play an essential role in decomposing dead plant material in tropical ecosystems and are thus major sources of gaseous C emissions in many environments. In African savannas, fungus-growing termites are among the ecologically most influential termite species. We studied the gas exchange from mounds of two closely related fungus-growing species (Macrotermes subhyalinus and M. michaelseni, r

Diurnal migration patterns in willow warblers differ between the western and eastern flyways

It is a long-standing view that the main mechanism maintaining narrow migratory divides in passerines is the selection against intermediate and suboptimal migratory direction, but empirical proof of this is still lacking. We present novel results from a willow warbler migratory divide in central Sweden from where birds take the typical SW and SE as well as intermediate routes to winter quarters in

Specific and efficient peptide substrates for assaying the proteolytic activity of prostate-specific antigen

Prostate-specific antigen (PSA) is a serine protease secreted by hath normal prostate glandular celts and prostate cancer cells. The major proteolytic substrates for PSA are the gel-forming proteins in semen, semenogelin (Sg) I and II. On the basis of the PSA cleavage map for Sg I and II, a series of small peptides (ie., ≤ 7 amino acids) was synthesized and coupled at the COOH terminus to 7-amino-

Secretory Leucocyte Protease Inhibitor in the Male Genital Tract : PSA‐Induced Proteolytic Processing in Human Semen and Tissue Localization

ABSTRACT: Secretory leucocyte protease inhibitor, SLPI, is a low‐molecular‐weight, acid‐stable protein present in the liquid part of fresh human ejaculate but not demonstrable in the gel structure. No fragmentation of SLPI occurred during gel dissolution, but a slow proteolytic cleavage of SLPI was seen on incubation of the liquified semen at 37°C. The same pattern of degradation products was seen

Model-based assessment of boiling heat transfer enhanced by coatings

The present study aims to investigate boiling heat transfer enhancement on coated surfaces, based on mechanistic models. An electrochemical deposition method was used to fabricate coatings on copper surfaces which enhance critical heat flux and heat transfer coefficient of deionized water by 35.5% and 40.1%, respectively, compared with a smooth surface. Bubble dynamics indicates that regardless of

Unilateral or Bilateral Retroperitoneal Lymph Node Dissection in Nonseminoma Patients with Postchemotherapy Residual Tumour? Results from RETROP, a Population-based Mapping Study by the Swedish Norwegian Testicular Cancer Group

BACKGROUND: The distribution of retroperitoneal lymph node metastases for patients with nonseminoma and a residual tumour of 10-49 mm in a population-based setting is unknown. This information is needed to justify selection of patients for a unilateral template resection. OBJECTIVE: To describe the location of retroperitoneal metastases and recurrences in patients with nonseminoma germ cell tumour

Inflammatory potential of diet and risk of nonalcoholic fatty liver disease : a prospective cohort study

Background/objectives: Diet is an important factor that can exacerbate or ameliorate chronic inflammation, which has been implicated in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). However, no prospective study has yet investigated the relation between the inflammatory potential of diet and NAFLD. The aim of this study was to investigate the association between the inflammatory po

Homotopic contralesional excitation suppresses spontaneous circuit repair and global network reconnections following ischemic stroke

Understanding circuit-level manipulations that affect the brain’s capacity for plasticity will inform the design of targeted interventions that enhance recovery after stroke. Following stroke, increased contralesional activity (e.g. use of the unaffected limb) can negatively influence recovery, but it is unknown which specific neural connections exert this influence, and to what extent increased c